The expression of CK4, CK13 in hypopharyngeal squamous cell carcinomas and its clinical significance
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摘要: 目的：探讨喉咽鳞状细胞癌组织中细胞角蛋白13（CK13）和细胞角蛋白4（CK4）的表达与喉咽鳞状细胞癌临床病理特征的关系。方法：应用免疫组织化学方法检测56例喉咽鳞状细胞癌患者的癌组织标本及癌旁正常组织中CK13及CK4的蛋白表达，应用VIDAS图像分析仪分析其图像的积分光密度，并进行统计学处理。结果：CK4与CK13在癌旁正常组织中均呈强阳性表达，而在喉咽鳞状细胞癌组织中的表达下降（P<0.01），且与病理分化程度均呈正相关（P<0.05）。CK4在T1、T2期的表达最强，在T3、T4期的表达依次减弱（F=93.814，P<0.05）；CK4在Ⅰ~Ⅱ期的表达最强，在Ⅲ期、Ⅳ期中的表达逐渐减弱（F=127.894，P<0.05）；CK4在无淋巴结转移的鳞状细胞癌组织中的表达较强，而在有淋巴结转移的鳞状细胞癌组织中表达较弱（T=10.678，P<0.05）。而CK13与肿瘤大小、临床分期及有无淋巴结转移无显著相关。结论：CK4与CK13共同检测对喉咽鳞状细胞癌的早期诊断、手术方法的选择以及判断预后具有指导意义。Abstract: Objective: To investigate the expressions of cytokeratin (CK) 4 and 13 in hypopharyngeal squamous cell carcinomas(HSCC) and to analyse their relationship with the clinicopathologic features. Method: The expressions of CK4 and CK13 were detecteded by immunohistochemistry in the cancer tissues and pericarcinomatous tissues of 56 cases with HSCC. Then the integral optical density (iOD) was measured by VIDAS image analyser and the data were statistically treated by SPSS 13.0. Result: The expressions of CK4 and CK13 were significantly higher in pericarcinomatous tissues than those in cancerous tissues of HSCC(P<0.01). Morever, the expression levels of CK4 and CK13 were positively related to the extent of differentiation of HSCC (P<0.05). The expression of CK4 were stonger in T1-T2, stageⅠ-Ⅱ and N0 than in T3-T4, stage Ⅲ-Ⅳ and N+ (F=93.814, F=127.894, and T=10.678 respectively, all P<0.05). However the expression of did not correlated to the tumor size, clinical stage and N stage in HSCC.Conclusion: The detection of CK4 and CK13 might play an important role in the early diagnosis, surgical procedure selection and prognostic prediction of HSCC.
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[1] TAKES R P,STROJAN P,SILVER C E,et al.Current trends in initial management of hypopharyngeal cancer:the declining use of open surgery[J]. Head Neck,2012,34:270-281.
[2] EDGE S, BYRD D R,COMPTON C C,et al. AJCC cancer staging handbook[M].7th ed.Chicago:Springer,2010:129-144.
[3] TAKIKITA M, HU N, SHOU J Z,et al. Fascin and CK4 as biomarkers for esophageal squamous cell carcinoma[J].Anticancer Res,2011,31:945-952.
[4] YANAGAWA T, YOSHIDA H, YAMAGATA K, et al. Loss of cytokeratin 13 expression in squamous cell carcinoma of the tongue is a possible sign for localrecurrence[J]. J Exp Clin Cancer Res,2007,26:215-220.
[5] KLIJANIENKO J, MICHEAU C, CARLU C, et al.Significance of keratin 13 and 6 expression in normal, dysplasic and malignant squamous epithelium of pyriform fossa[J].Virchows Arch A Pathol Anat Histopathol,1989,416:121-124.
[6] 丁凯,毛志福,孙作永.食管鳞状细胞癌中细胞角蛋白13的表达及临床意义[J].疑难病杂志,2010, 9(10):746-748.
[7] 贺光,富伟能,邱广斌,等.细胞角蛋白基因13在喉鳞状细胞癌中缺失和表达的研究[J].遗传学报,2002,29(5):390-395.
[8] QIU Y, TIAN Y, XIAO J.[The role of cytokeratin 13 gene in human nasopharyngeal carcinoma] [J]. Zhonghua Zhong Liu Za Zhi,1999,21:444-446.
[9] CHUNG J Y, BRAUNSCHWEIG T, HU N, et al. A multiplex tissue immunoblotting assay for proteomic profiling:a pilot study of the normal to tumor transition of esophageal squamous cell carcinoma[J]. Cancer Epidemiol Biomarkers Prev,2006,15:1403-1408.
[10] XUE L Y, HU N, SONG Y M, et al. Tissue microarray analysis reveals a tight correlation between protein expression pattern and progression ofesophageal squamous cell carcinoma[J]. BMC Cancer,2006,22:296-309.
[11] VIAENE A I, BAERT J H. Expression of cytokeratin-mRNAs in squamous-cell carcinoma and balloon-cell formation of human oesophageal epithelium[J]. Histochem J,1995,27:69-78.
[12] TAKIKITA M, HU N, SHOU J Z,et al. Fascin and CK4 as biomarkers for esophageal squamous cell carcinoma[J]. Anticancer Res,2011,31:945-952.
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