低氧对人喉癌Hep-2细胞增殖及凋亡的影响

胡艳红; 李晓明

doi:10.13201/j.issn.1001-1781.2014.12.011

低氧对人喉癌Hep-2细胞增殖及凋亡的影响

胡艳红¹,
李晓明^2, ,

- 1. 唐山市协和医院耳鼻咽喉头颈外科(河北唐山, 063000);
- 2. 白求恩国际和平医院耳鼻咽喉头颈外科

详细信息

通讯作者: 李晓明,E-mail:xmlmo@126.com

中图分类号: R739.65

收稿日期: 2013-09-01

Effects of hypoxia on cell proliferation and apoptosis in human laryngeal cancer Hep-2 cells

HU Yanhong¹,
LI Xiaoming^2, ,

- 1. Department of Otolaryngology Head and Neck Surgery, Tangshan Xiehe Hospital, Tangshan, 063000, China;
- 2. Department of Otolaryngology Head and Neck Surgery, Bethune International Peace Hospital

More Information

Corresponding author: LI Xiaoming, E-mail: xmlmo@126.com

Received Date: 01 September 2013

摘要

摘要: 目的：探讨低氧对喉癌Hep-2细胞增殖及凋亡的影响。方法：模拟人体实体肿瘤低氧环境，应用MTT法检测低氧组与常氧组6、12、24、36 h细胞增殖率，流式细胞学方法检测低氧组与常氧组6、12、24、36 h细胞凋亡率。结果：常氧与低氧细胞增殖率随时间延长均呈逐渐升高趋势；常氧组与低氧组6、12、24、36 h各时间点下比较于24 h差异有统计学意义（P<0.01）。低氧组细胞凋亡率于6、12、24 h较常氧组低（P<0.01），于36 h较常氧组高（P<0.05）。结论：低氧可促进细胞增殖，且细胞增殖率可随时间延长逐渐增高。低氧能够抑制细胞凋亡，但持续缺氧还可以促进细胞凋亡。
- 低氧 /
- 增殖 /
- 凋亡 /
- 喉肿瘤
Abstract: Objective: To explore the effects of hypoxia on cell proliferation and apoptosis in human laryngeal cancer Hep-2 cells. Method: This experiment simulates the hypoxia environment of human solid tumor. Apoptosis rate and proliferation rate were detected by flow cytometry and MTT respectively. Both were measured under both the hypoxia and normoxia conditions at 6 h, 12 h, 24 h and 36 h, respectively.Result: The proliferation rate showed a trend of gradual increase under both hypoxia and normoxia conditions. The proliferation rate at 6 h, 12 h, and 36 h respectively were significant different from that at 24 h under both conditions (P<0.01). The apoptosis rate in hypoxic group were significant lower that in normoxic group at 6 h, 12 h, 24 h, respectively (P<0.01), while the opposite happened at 36 h (P<0.05).Conclusion: Hypoxia can promote cell proliferation, and cell proliferation rate can be increased gradually with time.Hypoxia can inhibit apoptosis, but persistent hypoxia may promote cell apoptosis.
- hypoxia /
- proliferation /
- apoptosis /
- laryngeal neoplasms

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