Expression of Th1/Th2 transcription factors and cytokines in laryngeal sqamous cell carcinoma and its clinical significance
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摘要: 目的:从转录因子与细胞因子的角度分析喉鳞状细胞癌患者Th1/Th2细胞分化趋势,探讨T-bet/GATA3与p53的相关性,了解其与肿瘤转移之间的关系。方法:利用荧光定量PCR技术检测49例喉鳞状细胞癌患者(实验组)及30例正常人(正常对照组)外周血单个核细胞(PBMC)中T-bet、GATA3和IFN-γ、IL-4 mRNA的水平;应用免疫组织化学法检测实验组中p53的表达状况。结果:实验组T-bet、GATA3、IFN-γ、IL-4的表达率依次为42.86％(21/49)、71.43％(35/49)、26.53％(13/49)、63.27％(33/49)。定量PCR结果显示,实验组的T-bet和IFN-γ的表达明显低于正常对照组(P<0.05),而GATA3和IL-4的表达则明显高于正常对照组(P<0.05)。在p53阳性的患者中,T-bet的表达量较低,而GATA3的表达量较高,且T-bet和GATA3的表达与p53具有相关性。结论:喉鳞状细胞癌患者有明显的Th2漂移现象,且T-bet与GATA3可作为反映肿瘤转移的参考指标。Abstract: Objective: To study the differentiation of Th1/Th2 on the levels of transcription factors and cytokines production in patients with laryngeal sqamous cell carcinoma in peripheral blood mononuclear cells(PBMC).In addition,the relation of p53 and T-bet,GATA3 expression was discussed for understanding the role of T-bet and GATA3 in metastasis.Method: The gene expression of Th1/Th2 type transcription factors T-bet,GATA3 and cytokines IFN -γ,IL-4 were determined by RT-PCR and realtime RT-PCR from 49 patients with laryngeal sqamous cell carcinoma and 30 normal controls. The expression of p53 was analyzed by immunohistochemistry.Result: Predominant expression of Th2 type cytokines was detected in 49 laryngeal sqamous cell carcinoma patients.The expression rates of T-bet,GATA3,IFN-γ and IL-4 was 42.86％(21/49),71.43％(35/49),26.53％(13/49),63.27％(33/49) respectively.The expression rates of T-bet and IFN-γ in patients were lower than in control group(P<0.05),but the results of GATA3 and IL-4 were opposite(P<0.05).The similar results were obtained by realtime PCR.The expression of p53 in patients was accompanied with lower expression of T-betmRNA and higher expression of GATA3mRNA.Conclusion: There is predominant expression of Th2 type transcription factors and cytokines in PBMC of laryngeal sqamous cell carcinoma patients.T-bet and GATA3 can be used as reference indicators for the metastasis of laryngeal sqamous cell carcinoma.
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Key words:
- laryngeal sqamous cell carcinoma /
- Th1/Th2 /
- transcription factors /
- cytokines
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[1] TAN J Q, XIAO W, WANG L, et al. Type I natural killer T cells: naturally born for fighting[J]. Acta Pharmacol Sin,2010,31:1123-1132.
[2] ITO N,NAKAMURA H,TANAKA Y,et al.Lung carcinoma:analysis of T helper type 1 and 2 cells and T cytotoxic type 1 and 2 cells by intracellular cytokine detection with flow cytometry[J]. Cancer, 1999,85:2359-2367.
[3] STABO S J,KIM S T,COATA B L,et al. A novel transcription actor,T-bet,directs Th1 lineage commitment[J]. Cell,2000,100:655-559.
[4] SUNDRUD M S, NOLAN M A. Synergistic and combinatorial control of T cell activation and differentiation by transcription factors[J]. Curr Opin Immunol, 2010,22:286-292.
[5] RENGARAJAN J,SZABO S J,GLIMCHER L H. Transcriptional regulation of Th1/Th2 polarization[J]. Immunology Today,2000,21:479-483.
[6] KAWASHIMA M,MIOSSEC P. mRNA quantification of T-bet, GATA-3,IFN-γ,IL-4 shows a defective Th1 immune response in the pripheral blood from rheumatoid arthritis patients;link with disease activity[J].J Clin Immunol,2005,25:209-214.
[7] PENG S L,TOWNSEND M J,HECHT J L,et al. T-bet regulates metastasis rate in a murine model of primary prostate cancer[J]. Cancer Res,2004,64:452-455.
[8] 李漫雪,蔡群峰. 声门上型喉癌手术边缘的分子学研究[J].临床耳鼻咽喉头颈外科杂志,2009,23(17):802-804.
[9] 强笔,马纪清,邱杰,等.HPV16/18-E6,P53和MDM2蛋白在人喉磷癌中的表达及相关性研究[J].第二军医大学学报,2006,27(12):1320-1323.
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