程序性细胞死亡因子4在喉鳞状细胞癌中的表达及其与肿瘤细胞增殖和凋亡的关系

王建亭; 张玉祥

doi:10.13201/j.issn.1001-1781.2012.06.004

程序性细胞死亡因子4在喉鳞状细胞癌中的表达及其与肿瘤细胞增殖和凋亡的关系

王建亭^1,2, ,,
张玉祥³

- 1 首都医科大学附属北京朝阳医院耳鼻咽喉头颈外科首都医科大学耳鼻咽喉科学院耳鼻咽喉头颈科学教育部重点实验室(北京, 100020);
- 2 首都医科大学基础医学院生物化学与分子生物学系
基金项目:
首都医科大学基础-临床科研合作基金项目(No:09JL-L09)

详细信息

通讯作者: 王建亭,E-mail:tingjianwang@yahoo.com.cn

中图分类号: R739.6

收稿日期: 2011-12-21

Expression of programmed cell death 4 and its correlation with proliferation and apoptosis in laryngeal squamous cell carcinoma

WANG Jianting^1,2, ,,
ZHANG Yuxiang³

- 1 Department of Otorhinolaryngology-Head and Neck Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China;
- 2 Department of Biochemistry and Molecular Biology, Capital Medical University

More Information

Corresponding author: WANG Jianting, E-mail: tingjianwang@yahoo.com.cn

Received Date: 21 December 2011

摘要

摘要: 目的:探讨喉鳞状细胞癌中程序性细胞死亡因子4(PDCD4)蛋白表达与肿瘤细胞增殖和凋亡的关系及其临床意义。方法:采用免疫组织化学技术检测60例喉鳞状细胞癌组织及21例正常喉黏膜上皮组织中PDCD4和Ki-67的表达,原位末端标记(TUNEL)法检测细胞凋亡,以细胞增殖指数(PI)和细胞凋亡指数(AI)表示增殖与凋亡。结果:喉鳞状细胞癌组织中PDCD4蛋白阳性表达率低于癌旁正常喉黏膜组织(P＜0.01),喉鳞状细胞癌组织中PDCD4蛋白阳性表达和喉鳞状细胞癌临床分型(肿瘤部位)、TNM分期无关,与病理分级、淋巴结转移有关(P＜0.05);喉鳞状细胞癌组织细胞PI明显高于癌旁正常喉黏膜组织(P＜0.01), 细胞PI与喉鳞状细胞癌临床分型(肿瘤部位)、病理分级、淋巴结转移无关,与TNM分期有关(P＜0.05);喉鳞状细胞癌组织细胞AI明显高于癌旁正常喉黏膜组织(P＜0.01), 细胞AI与喉鳞状细胞癌临床分型(肿瘤部位)、TNM分期、淋巴结转移无关,与病理分级有关(P＜0.01);喉鳞状细胞癌PDCD4阳性表达的细胞PI低于阴性组(P＜0.05),细胞AI高于阴性组(P＜0.05)。结论:PDCD4在喉鳞状细胞癌中低表达,可能与喉鳞状细胞癌细胞的增殖和凋亡均有一定的关联。
- 喉肿瘤 /
- 鳞状细胞癌 /
- 凋亡程序性细胞死亡因子4 /
- 增殖 /
- 凋亡
Abstract: Objective: To study the expression of PDCD4 and its relationship with cell proliferation and apoptosis in laryngeal squamous cell carcinomas and to evaluate its clinical significance.Method: The immunohistochemical staining method was applied to investigate the expression of PDCD4 and Ki-67 in 60 laryngeal squamous cell carcinomas tissues and 21 their surrouding laryngeal normal mucosa tissues.TUNEL method was applied to detect the apoptosis.The proliferation index(PI) and apoptosis index(AI) were defined.Result: The positive rate of PDCD4 protein was significantly lower in laryngeal squamous cell carcinomas than that in the laryngeal normal mucosa tissues(P＜0.01).The expression of PDCD4 had relation with the differentiation grading and lymph node matastasis(P＜0.05),but not related to tumor sites and TNM stage. The PI of laryngeal squamous cell carcinomas tissues was higher than that of the normal laryngeal mucosa tissues(P＜0.01). No relation was found among PI and tumor sites, differentiation grading and lymph node metastasis.PI had correlation with TNM stage(P＜0.05).The AI of the cancer was higher than that of normal laryngeal mucosa tissues(P＜0.01). There was no relationship between AI and tumor sites, TNM stage and lymph node metastasis.AI correlated with differentiation grading(P＜0.01).The PI in PDCD4 positive group was significantly lower than in PDCD4 negative group(P＜0.05).The AI in PDCD4 positive group was significantly higher than in PDCD4 negative group(P＜0.05).Conclusion: The expression of PDCD4 is low in laryngeal squamous cell carcinomas and it may play important roles in occurrence and development of laryngeal squamous cell carcinomas through the inducing apoptosis and inhibiting proliferation.
- laryngeal neoplasms /
- squamous cell cancer /
- programmed cell death 4 /
- proliferation /
- apoptosis

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参考文献(11)

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