鼻息肉中诱导型一氧化氮合酶、Bax及Bcl-2的表达及相关性研究

刘燕美; 马永明; 钱海; 吴卫疆

doi:10.13201/j.issn.1001-1781.2012.06.014

鼻息肉中诱导型一氧化氮合酶、Bax及Bcl-2的表达及相关性研究

- 1 江苏大学附属人民医院耳鼻咽喉科(江苏镇江, 212002);
- 2 江苏大学基础医学与医学技术学院

详细信息

通讯作者: 马永明,E-mail:maym121@163.com

中图分类号: R765.25

收稿日期: 2011-08-14

The expression and correlation of inducible nitric oxide synthase,Bax and Bcl-2 in nasal polyps

- 1 Department of Otolaryngology, Affiliated People's Hospital of Jiangsu University, Zhenjiang, 212002, China;
- 2 Preclinical Medical College, Jiangsu University

More Information

Corresponding author: MA Yongming, E-mail: maym121@163.com

Received Date: 14 August 2011

摘要

摘要: 目的:检测鼻息肉组织中诱导型一氧化氮合酶(iNOS)和凋亡相关基因Bax、Bcl-2的表达及相关性。方法:30例鼻息肉组织,应用TUNEL方法检测其凋亡情况;免疫组织化学SABC法检测iNOS及Bax、Bcl-2的表达;western blot方法检测iNOS、Bax、Bcl-2的表达。结果:①TUNEL方法检测凋亡细胞位于鼻息肉表层上皮细胞及腺体上皮细胞,均为弱阳性。②免疫组织化学法检测iNOS、Bax、Bcl-2均表达于鼻息肉组织上皮细胞、腺上皮细胞、血管内皮细胞和浸润的炎性细胞,阳性表达定位于细胞质,其中Bax表达为弱阳性,iNOS、 Bcl-2表达为强阳性。③Western blot检测3种蛋白均有表达条带,iNOS、Bal-2条带清晰,Bax条带软弱,差异有统计学意义(P<0.01);iNOS 与Bcl-2的灰度值比值呈正相关(r=0.851,P<0.01);iNOS与Bax的灰度值比值呈负相关(r=-0.714,P<0.01)。结论:鼻息肉组织中存在抗凋亡和促凋亡2种蛋白,iNOS抑制细胞凋亡,可能对鼻息肉的发生、发展起重要的促进作用。
- 鼻息肉 /
- 一氧化氮合酶 /
- 凋亡 /
- Bax /
- Bcl-2
Abstract: Objective: To detect the expressions of inducible nitric oxide synthase(iNOS),apoptosis-related gene Bax,Bcl-2 in nasal polyps,and discuss the their relationship.Method: The apoptosis of 30 cases of nasal polyps was detected by TUNEL assay. The expressions of iNOS and Bax,Bcl-2 was detected by immunohistochemical SABC method. The expressions of iNOS and Bax,Bcl-2 was measured by western blot.Result: ① The weakly positive stained apoptotic cells were detected at the surface epithelial cells and glandular epithelial cells of nasal polyps by TUNEL assay.②Immunohistochemical method revealed that positive stainings of iNOS,Bax,Bcl-2 located in the cytoplasm of epithelial cells, glandular epithelial cells, endothelial cells and infiltrating inflammatory cells in nasal polyps. The expression of Bax was weak, while the expressions of iNOS and Bcl-2 were strong.③iNOS, Bcl-2 and Bax was detected by western blot. The expressions of these proteins were significantly different(P<0.01). The expression of iNOS and Bcl-2 had a positive correlation(r=0.851,P<0.01), while the expression of iNOS and Bax had a negative correlation(r=-0.714,P<0.01).Conclusion: The pro-apoptotic and anti-apoptotic proteins are co-existed in the nasal polyps. iNOS may play an important role in the pathogenesy of nasal polyps through inhibition of apoptosis.
- nasal polyps /
- inducible nitric oxide synthase /
- apoptosis /
- Bax /
- Bcl-2

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