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摘要: 目的 本文主要研究常年性变应性鼻炎儿童鼻腔微生物群的多样性，以及物种组成差异，并可能用于未来作为疾病进展及治疗的生物标志物。 方法 共纳入65例受试者，包括35例常年性变应性鼻炎组和30例健康对照组。收集受试者鼻腔拭子，采用16s-rDNA高通量测序技术检测鼻拭子微生物序列，利用生物信息学方法分析各组微生物多样性及物种组成差异。 结果 变应性鼻炎组的Alpha多样性指数高于对照组，主要表现在物种均匀度高于对照组，而物种丰富度无明显差异; Beta多样性指数2组间未见明显差异; 变应性鼻炎患儿鼻腔微生物中厚壁菌门中葡萄球菌属明显增高，而变形菌门中的莫拉克斯氏菌低于对照组。 结论 变应性鼻炎儿童鼻腔微生物多样性及物种组成与健康儿童存在差异，差异微生物如何与宿主相互作用，参与免疫调节及炎症反应，还需进一步研究。Abstract: Objective This paper focuses on the diversity of nasal microbiota in children with perennial allergic rhinitis and the differences in species composition, which may be used in the future as a biomarker for disease progression and treatment. Methods A total of 65 subjects were enrolled, including 35 perennial AR patients(AR group) and a Control group(CG group) of 30 children without AR. Collect basic information and examination reports of nasal swabs. 16S-rDNA high-throughput sequencing technology was used to detect the microbial sequence in nasal swabs, and the composition and difference of microbial diversity in each group were analyzed by bioinformatics methods. Results The Simpson and Shannon index of the alpha diversity in the AR group had a significantly increase compared to the CG group. Beta diversity was not different between the groups. Staphylococcus(Firmicutes) of the AR group were significantly higher than that of the CG group, but Moraxella is lower than that of the CG group. Conclusion Nasal microbial diversity and species composition of children with allergic rhinitis differ from those of healthy children, and how the differential microorganisms interact with the host and participate in immune regulation and inflammatory response requires further study.
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Key words:
- allergic rhinitis /
- child /
- microbial community
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