The expression and significance of Piezo1 in chronic rhinosinusitis with nasal polyps
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摘要: 目的 探索机械敏感离子通道蛋白Piezo1、钙黏蛋白E和波形蛋白在鼻息肉中的表达和临床意义。方法 纳入全身麻醉下行鼻内镜手术的患者35例，其中鼻息肉患者20例(息肉组)，主诉鼻塞或鼻出血的单纯鼻中隔偏曲患者15例(对照组)。分别采用免疫荧光和蛋白免疫印迹法检测Piezo1、钙黏蛋白E和波形蛋白在鼻息肉组织及其来源的鼻原代上皮细胞中蛋白表达水平。应用人TGF-β1蛋白在人支气管上皮BEAS-2B细胞系建立体外上皮间质转化(epithelial mesenchymal transition，EMT)模型，运用实时荧光定量PCR检测Piezo1、钙黏蛋白E和波形蛋白的基因表达水平。结果 与对照组比较，鼻息肉组织及鼻原代上皮细胞中Piezo1与波形蛋白表达水平均升高(P<0.05)，钙黏蛋白E表达降低(P<0.05)。在体外EMT模型中，Piezo1和波形蛋白均上调，钙黏蛋白E明显下调。结论 Piezo1在鼻息肉中表达升高，与EMT标志物波形蛋白趋势相同，钙黏蛋白E趋势相反。这表明Piezo1参与鼻息肉EMT过程，与鼻息肉发生发展相关。Abstract: Objective To explore the expression and importance of Piezo1, E-cadherin, and Vimentin in nasal polyps patients.Methods Thirty-five patients undergoing endoscopic sinus surgery under general anesthesia were streamed into 20 cases of nasal polyps(NP group) and 15 cases of simple septoplasty without any sinus disease(Control group). Immunofluorescence staining and Western Blot were applied to detect the protein level of Piezo1, E-cadherin, and Vimentin in NP tissues and nasal polyp-derived primary human nasal epithelial cells(pHNECs). Also, BEAS-2B cell lines were treated with human TGF-β1 protein to establish epithelial mesenchymal transition(EMT) model in vitro and quantitative real-time polymerase chain reaction were used to calculate Piezo1 and above biomarkers in the model.Results Compared with control group, Piezo1 and Vimentin showed higher level while E-cadherin was lower in NP tissues and pHNECs.In EMT model in vitro, Piezo1 and Vimentin were demonstrated higher expression with decreased level of E-cadherin.Conclusion The tendency of Piezo1 is consistent with the mesenchymal-related biomarker Vimentin, going against with epithelial-related biomarker E-cadherin, implying its involvement with EMT process in nasal polyps.
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表 1 实时荧光定量PCR引物及退火温度
基因 序列(5’~3’) 退火温度/℃ E-cadherin F: TCACTGACACCAACGATAATCC
R: TTTCAGTGTGGTGATTACGACGTTA60 Piezo1 F: TGGAGGAGGCTGGCATCATCTG
R: GACGTGCAGGTAGTAATGGCTAAGG60 Vimentin F: CCGAAAACACCCTGCAATCTTTC
R: CACATCGATTTGGACATGCTGT60 GAPDH F: TGACATCAAGAAGGTGGTGAAGCAG
R: GTGTCGCTGTTGAAGTCAGAGGAG60 
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