鼻息肉VEGF-B基因启动子甲基化研究

谭海燕; 白伟良; 王枭维; 徐婷婷

doi:10.13201/j.issn.1001-1781.2017.19.005

鼻息肉VEGF-B基因启动子甲基化研究

- 中国医科大学附属盛京医院耳鼻咽喉科(沈阳, 110004)
基金项目:
辽宁省科学事业公益研究基金资助 (No:2013001010)

沈阳市科学计划项目资助 (No:F11-264-1-07, F13-220-9-21)

详细信息

通讯作者: 白伟良, E-mail:bweiliangcmu@163.com

中图分类号: R765.25

收稿日期: 2017-06-30

The exploration of VEGF-B methylation in the nasal polyp

- Department of Otolaryngology, Shengjing Hospital, the Affiliated Hospital of China Medical University, Shenyang, 110004, China

More Information

Corresponding author: BAI Weiliang, E-mail: bweiliangcmu@163.com

Received Date: 30 June 2017

摘要

摘要: 目的: 探讨VEGF-B基因启动子在鼻息肉中甲基化状态。方法: 应用MS-PCR检测28例实验组鼻息肉组织和12例对照组正常组织VEGF-B基因启动子甲基化状态,并对MS-PCR产物进行测序。结果: VEGF-B启动子甲基化在28例鼻息肉中有9例阳性,19例阴性;在12例对照组织中8例阳性,4例阴性。鼻息肉组织和对照组织中VEGF-B基因启动子甲基化阳性率的差异有统计学意义(χ²=4.096,P<0.05)。MS-PCR产物测序结果表明鼻息肉中存在VEGF-B基因启动子甲基化修饰。结论: 鼻息肉中VEGF-B基因启动子可能存在低甲基化状态。推测鼻息肉VEGF-B基因甲基化调控着VEGF-B的表达,在鼻息肉的发生机制中占有重要地位。
- 鼻息肉 /
- VEGF-B /
- 启动子 /
- 甲基化特异性聚合酶链反应
Abstract: Objective: The objective of this study is to investigate the methylation status of vascular endothelial cell growth factor B (VEGF-B) gene and to indentify the roles in pathogenesis, development and classification of nasal polyps.Method: The methylation status of VEGF-B gene of 28 nasal polyp tissues and 12 samples of inferior turbinate tissues were detected by methylationspecific-polymerase chain reaction (MS-PCR) and gene sequencing.Result: There was significant statistic diference between nasal polyp tissue group and control group (χ²=4.096, P<0.05). The results of gene sequencing suggest that the VEGF-B gene promoter were hypomethylation status in the nasal polyps.Conclusion: Methylation status of VEGF-B promoter may play an important role in the pathogenetic mechanism of nasal polyps.
- nasal polyps /
- vascular endothelial growth factor B /
- promoter /
- methylation specific-polymerase chain reaction

HTML全文

参考文献(14)

[1]	BOBIC S, HOX V, CALLEBAUT I, et al.Vascular endothelial growth factor receptor 1expression in nasal polyp tissue[J].Allergy, 2014, 69:237-245.
[2]	CURTIN J A, SIMPSON A, BELGRAVE D, et al.Methylation of IL-2promoter at birth alters the risk of asthma exacerbations during childhood[J].Clin Exp Allergy, 2013, 43:304-311.
[3]	CHO J S, KANG J H, HAN I H, et al.Activation of TLR4 induces VEGF expression via Akt pathway in nasal polyps[J].Clin Exp Allergy, 2013, 43:1038-1047.
[4]	DIVEKAR R D, SAMANT S, RANK M A, et al.Immunological profiling in chronic rhinosinusitis with nasal polyps reveals distinct VEGF and GM-CSF signatures during symptomatic exacerbations[J].Clin Exp Allergy, 2015, 45:767-778.
[5]	DEVAUX Y, VAUSORT M, AZUAJE F, et al.Low levels of vascular endothelial growth factor B predict left ventricular remodeling after acutemyocardial infarction[J].J Card Fail, 2012, 18:330-337.
[6]	CHOI M R, YOO N J, LEE S H, et al.Frameshift mutation of an angiogenesis factor VEGFB and its mutational heterogeneity incolorectal cancers[J].Pathol Oncol Res, 2015, 21:853-855.
[7]	FRUTH K, ZHU C, SCHRAMEK E, et al.Vascular endothelial growth factor expression in nasal polyps of aspirin-intolerant patients[J].Arch Otolaryngol Head Neck Surg, 2012, 138:286-293.
[8]	SEIBERLING K A, CHURCH C A, HERRING J L.Epigenetics of chronic rhinosinusitis and the role of the eosinophil[J].Int Forum Allergy Rhinol, 2012, 2:80-84.
[9]	ACEVEDO N, REINIUS L E, GRECO D, et al.Risk of childhood asthma is associated with CpG-site polymorphisms, regional DNA methylationand mRNA levels at the GSDMB/ORMDL3 locus[J].Hum Mol Genet, 2015, 24:875-890.
[10]	BRAND S, KESPER D A, TEICH R, et al.DNA methylation of TH1/TH2 cytokine genes affects sensitization and progress of experimental asthma[J].J Allergy Clin Immunol, 2012, 129:1602-1610.e6.
[11]	CAO Q, ZHANG T, ZHANG J, et al.Correlation analysis of STAT3 and VEGF expression and eosinophil infiltration in nasal polyps[J].Eur Arch Otorhinolaryngol, 2015, 272:1955-1960.
[12]	LINKE R, PRIES R, KNNECKE M, et al.Increased activation and differentiated localization of native and phosphorylated STAT 3in nasalpolyps[J].Int Arch Allergy Immunol, 2013, 162:290-298.
[13]	ALBASANZ-PUIG A, MURRAY J, NAMEKATA M, et al.Opposing roles of STAT-1and STAT-3in regulating vascular endothelial growth factorexpression in vascular smooth muscle cells[J].Biochem Biophys Res Commun, 2012, 428:179-184.
[14]	MICHEL S, BUSATO F, GENUNEIT J, et al.Farm exposure and time trends in early childhood may influence DNA methylation in genes related to asthma and allergy[J].Allergy, 2013, 68:355-364.