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摘要: 目的：分析吸烟相关喉鳞状细胞癌患者抗氧化酶类的表达情况，进一步探讨吸烟相关喉鳞状细胞癌的氧化应激机制。方法：连续收集2012-2015年收治的138例喉鳞状细胞癌患者和45例志愿者，根据吸烟指数和是否患病将其分为3组：A组（88例，重度吸烟喉鳞状细胞癌患者），B组（50例，不吸烟喉鳞状细胞癌患者），C组（45例，重度吸烟志愿者）。检测3组患者血清、癌组织及癌旁组织匀浆中过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（MDA）和NRF2的表达水平。结果：①A组中CAT、GSH-Px的表达水平显著低于B组（P<0.05），但高于C组（P<0.05）；②A组MDA的水平显著高于B组（P<0.05）和C组（P<0.01）；③喉鳞状细胞癌患者癌组织中NRF2普遍存在高表达，且其表达水平随着癌组织分化程度降低而升高（P<0.05）。结论：相比不吸烟患者，重度吸烟喉鳞状细胞癌患者将面临更加严重的氧化应激压力，喉鳞状细胞癌患者NRF2的表达水平与其病理分期有关。Abstract: Objective: To investigate the role of oxidative stress in smoking-related laryngeal squamous carcinoma through detecting the expression of antioxidant enzymes in smoking patients.Method: A total of 138 cases with laryngeal squamous cell carcinoma enrolled in the first hospital affiliated the northern he bei college from 2012 to 2015 and forty five volunteers were conducted. All participants were divided into three groups according to smoking index:group A(heavy smoking, 88 cases of laryngeal cancer patients) and group B(no smoking 50 cases of laryngeal cancer patients) and C group(45 heavy smoking volunteers).Catalase(CAT), glutathione peroxidase(GSH-px) and malondialdehyde(MDA) and the expression of NRF2 in serum, tissue adjacent to carcinoma, and carcinoma tissues from each groups were measured, respectively.Result: ①the expression of the CAT and GSH-px in group A were significantly lower than that of group B(P<0.05), but higher than that of group C(P<0.05);②the MDA level of group A is significantly higher than group B(P<0.05) and C group(P<0.01);③NRF2 was highly expressed in carcinoma tissues, and the expression level was negatively correlated with degree of carcinoma differentiation (P<0.05).Conclusion: Compared with nonsmoking patients, heavy smoking patients with laryngeal cancer were under more severe oxidative stress. NRF2 expression level in patients with laryngeal squamous cell carcinomas was associated with pathological stage.
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Key words:
- antioxidant enzymes /
- smoking /
- laryngeal squamous cell carcinoma /
- oxidative stress
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