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摘要: 目的:观察甲醛对小鼠变应性鼻炎(AR)的影响。方法:48只雄性BALB/c小鼠随机分成6组,每组8只,其中A组为正常对照组;B组为单纯AR组;C组为甲醛暴露组;D、E和F组分别为甲醛低、中、高浓度(1.5、3.0、6.0 mg/m3)AR组。采集外周血间接酶联免疫吸附试验法测血清中IL-4、IL-10和干扰素γ(IFN-γ)的水平,并取鼻黏膜行苏木精-伊红染色观察鼻黏膜中嗜酸粒细胞浸润情况。结果:气态甲醛暴露可导致AR小鼠挠鼻和喷嚏次数增加;B、D、E和F组小鼠外周血中IL-4和IL-10水平较A组明显升高(P<0.05),D、E和F组较C组明显升高(P<0.05);而E、F组IL-4水平较B组明显升高(P<0.05),D、E和F组IL-10水平明显高于B组(P<0.05)。血清中IFN-γ水平B、D、E、F小鼠明显低于A组,B组血清中IFN-γ水平明显高于F组而低于C组,与C组相比D、E、F组外周血IFN-γ表达明显降低。鼻黏膜苏木精-伊红染色显示甲醛暴露可使AR小鼠鼻黏膜下嗜酸粒细胞数量增多。结论:气态甲醛暴露可促进Th2细胞因子表达,加重鼻黏膜中嗜酸粒细胞炎症反应,协同致敏原加重小鼠AR的症状。Abstract: Objective: To observe the effect of formaldehyde inhalation on the allergic rhinitis mice model.Method: Forty-eight male BALB/C mice in six experimental group were exposure to (A) saline control; (B) Der p1; (C) formaldehyde (3.0 mg/m3); (D) Derp1+formaldehyde (1.5 mg/m3); (E) Der p1+ formaldehyde (3.0 mg/m3); (F) Der p1+ formaldehyde (6.0 mg/m3).The concentrations of IL-4,IL-10 and IFN-γ in the peripheral serum were measured by enzyme-linked immunosorbent assay(ELISA). Nasal mucosal inflammation was evaluated by HE staining.Result: Formaldehyde exposure could increase the number of allergic rhinitis mice with sneezing and rubbing nose. The levels of IL-4 and IL-10 in group B, D, E and F were higher than that ingroup A (P<0.05). Compared with the group C, the group D, E and F could effectively increase serum IL-4 and IL-10. The concentration of IL-4 in group E and F was higher than that of group B, while the group C was lower (P<0.05). The concentration of IL-10 in group D, E and F was higher than that in group B (P<0.05). The expression of IFN-γ in group B, D, E and F was lower than that in group A.While, the IFN-γ expression in group B was lower than that of group C and higher than that in group F (P<0.05). Moreover, the concentration of IFN-γ in group D, E and F was lower compared with group C (P<0.05). The nasal mucosa HE staining showed that the density of EOS increased simultaneously in formaldehyde exposure allergic rhinitis groups.Conclusion: The study showed that formaldehyde exposure can promote Th2 cytokines and eosinophil infiltration and then aggravate the allergic rhinitis symptoms.
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Key words:
- rhinitis,allergic /
- formaldehyde /
- air pollution /
- Th1/Th2 imbalance
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[1] ROTIROTI G, SCADDING G K. Allergic rhinitis-an overview of a common disease[J]. Paediatrics Child Health, 2012, 22:287-292.
[2] ZHANG Y,ZHANG L. Prevalence of allergic rhinitis in china[J]. Allergy Asthma Immunol Res,2014,6:105-113.
[3] GREINER A N,HELLINGS P W,ROTIROTI G,et al. Allergic rhinitis[J]. Lancet,2011,378:2112-2122.
[4] WANG L,QU F,ZHANG Y,et al. Home environment in relation to allergic rhinitis among preschool children in beijing, china: a cross-sectional study[J]. Building Environment,2015,2:1-10.
[5] LIANG W, YANG X. Indoor formaldehyde in real buildings: Emission source identification, overall emission rate estimation, concentration increase and decay patterns[J]. Building Environment, 2013, 69:114-120.
[6] JEONG S H,KIM J H,SON B K,et al. Comparison of air pollution and the prevalence of allergy-related diseases in Incheon and Jeju City[J]. Korean J Pediatr,2011,54:501-506.
[7] 石文丹,许昱,陶泽璋,等.屋尘螨变应原Der p1DNA疫苗的构建及鼻黏膜免疫的研究[J]. 中华耳鼻咽喉头颈外科杂志, 2013,48(1):26-31.
[8] WU Y,YOU H,MA P.Role of transient receptor potential ion channels and evoked levels of neuropeptides in a formaldehyde-induced model of asthma in BALB/c mice[J]. PloS One,2013,8:e62827-e62827.
[9] CEUPPENS J. Western lifestyle, local defenses and the rising incidence of allergic rhinitis[J]. Acta Otorhinolaryngol Belg,2000,54:391-395.
[10] TAO H,FAN Y,LI X,et al. Investigation of formaldehyde and TVOC in underground malls in Xi'an, China: Concentrations, sources, and affecting factors[J]. Building Environment, 2015, 85:85-93.
[11] LIANG W,YANG X. Indoor formaldehyde in real buildings: Emission source identification, overall emission rate estimation, concentration increase and decay patterns[J]. Building Environment,2013,69:114-120.
[12] HU P, WEN S,LIU Y,et al. Carbon isotopic characterization of formaldehyde emitted by vehicles in Guangzhou, China[J]. Atmospheric Environment,2014,86:148-154.
[13] RUMCHEV K B,SPICKETT J T,BULSARA M K,et al. Domestic exposure to formaldehyde significantly increases the risk of asthma in young children[J]. Eur Respir J,2002,20:403-408.
[14] TAKAHASHI S,TSUJI K,FUJII K,et al. Prospective study of clinical symptoms and skin test reactions in medical students exposed to formaldehyde gas[J]. J Dermatol,2007,34:283-289.
[15] QIAO Y,LI B,YANG G,et al.Irritant and adjuvant effects of gaseous formaldehyde on the ovalbumin-induced hyperresponsiveness and inflammation in a rat model[J]. Inhal Toxicol,2009,21:1200-1207.
[16] 中华耳鼻咽喉头颈外科杂志编委会鼻科组,中华医学会耳鼻咽喉头颈外科学分会鼻科学组.变应性鼻炎诊断和治疗指南(2009年,武夷山)[J]. 中华耳鼻咽喉头颈外科杂志,2009,44(12): 977-978.
[17] ARICAN R Y,SAHIN Z,USTUNEL I,et al. Effects of formaldehyde inhalation on the junctional proteins of nasal respiratory mucosa of rats[J].Exp Toxicol Pathol,2009,61:297-305.
[18] LIN C C,LEE I T,YANG Y L,et al. Induction of COX-2/PGE(2)/IL-6 is crucial for cigarette smoke extract-induced airway inflammation: Role of TLR4-dependent NADPH oxidase activation[J]. Free Radic Biol Med,2010,48:240-254.
[19] MICHAEL S,MONTAG M,DOTT W. Pro-inflammatory effects and oxidative stress in lung macrophages and epithelial cells induced by ambient particulate matter[J].Environ Pollut,2013,183:19-29.
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