C/EBPα和CK10在鼻腔鼻窦内翻性乳头状瘤中的表达及临床意义

袁益兵; 孟宪影; 邬旭

doi:10.13201/j.issn.1001-1781.2015.05.015

C/EBPα和CK10在鼻腔鼻窦内翻性乳头状瘤中的表达及临床意义

- 大连医科大学第二附属医院耳鼻咽喉科(辽宁大连, 116027)
基金项目:
辽宁省自然科学基金(鼻腔鼻窦内翻性乳头状瘤的相关研究)

详细信息

通讯作者: 邬旭,E-mail:wu-xu2008@sohu.com

中图分类号: R739.6

收稿日期: 2014-11-09

Expression and significance of C/EBPα and CK10 in nasal inverted papilloma

- Department of Otolaryngology, the Second Hospital of Dalian Medical University, Dalian, 116027, China

More Information

Corresponding author: WU Xu, E-mail: wu-xu2008@sohu.com

Received Date: 09 November 2014

摘要

摘要: 目的: 检测鼻腔鼻窦内翻性乳头状瘤(NIP)组织中CCAAT增强子结合蛋白α(C/EBPα)、CK10的表达,进一步探讨其在NIP发生、发展及复发中的作用。方法: 应用免疫组织化学PV-6000二步法检测30例NIP组织、20例鼻息肉(NP)组织和10例正常鼻黏膜(NM)组织中C/EBPα、CK10的表达。结果: ①NM、NP与NIP组织中C/EBPα、CK10的阳性表达均差异有统计学意义(均P<0.05);②单纯NIP组与不典型增生NIP组中C/EBPα、CK10的阳性表达差异有统计学意义(P<0.05);③复发NIP组与未复发NIP组中C/EBPα、CK10的阳性表达率差异有统计学意义(P<0.05);④NIP组中C/EBPα和CK10的表达呈正相关(r=0.578,P<0.01)。结论: C/EBPα、CK10在NIP上皮增殖与分化的调控中可能起了重要作用,C/EBP-α和CK10的高度表达可作为NIP复发的指标。
- 鼻内翻性乳头状瘤 /
- CCAAT增强子结合蛋白α /
- CK10 /
- 复发
Abstract: Objective: The expression of C/EBPα,CK10 in nasal inverted papilloma (NIP) were detected in the study.Further discussed their significance in genesia,development and recurrence of NIP.Method: Three groups including nasal cavity mucosae (NM 10 cases),nasal polyp (NP 20 cases) and NIP (30 cases) were selected in the study.Expretion of C/EBPα,CK10 were detected by immunohistochemisty PV-6000 method.Result: ① The different expression of C/EBPα and CK10 in the group of NM,NP and NIP was statistically significant(P<0.05).②The different expression of C/EBPα,CK10 in the group of benign NIP and NIP with atypical hyperplasia was statistically significant(P<0.05). ③The different expression of C/EBPα and CK10 in the group of NIP with recurrence and NIP with no recurrence was statistically significant, P<0.05,respectively. ④Our result indicate that the relationship of C/EBPα and CK10 (r=0.578,P<0.01) was direct correlation.The difference was statistically significant.Conclusion: In conclusion, the present results describe C/EBPα,CK10 expression in NIP and their possible implication in the regulation of tumor growth and differentiation. C/EBPα and CK10 production may prove useful in terms of a prognostic marker for the recurrence in nasal inverted papilloma.
- nasal inverted papilloma /
- C/EBPα /
- CK10 /
- recurrence

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参考文献(14)

[1]	LESPERANCE M M,ESCALMADO R M.Sqquamous cell carcinoma arising in invented papilloma[J].Laryngoscope,1995,105:178-183.
[2]	HANKEY W,SILVER M,SUN B S,et al.Differential effects of sumoylation on the activities of CCAAT enhancer binding protein alpha(C/EBPα)p42 versus p30 may contribute in part,to aberrant C/EBPα activity in acute leukemias[J].Hematol Rep,2011,3:e5-e5.
[3]	MULLER C.Nucleolar retention of a translational C/EBPα is oform stimulates rDNA transcription and cell size[J].EMBO J,2010,29:897-909.
[4]	GERY S,TANOSAKI S,BOSE S,et al.Down-regulation and growth inhibitory role of C/EBPA in breast cancer[J].Clin Cancer Res,2005,11:3184-3490.
[5]	LOOMIS K D,ZHU S,YOON K,et al.Genetic ablation of CCAAT/enhancer binding protein alpha in epidermis reveals its role in suppression of epithelial tumorigenesis[J].Cancer Res,2007,67:6768-6776.
[6]	SHABANA E H,DEPONDT J,HOURSEAU M,et al.Production and significance of CCAAT enhancer binding proteins alpha and beta in sinonasal inverted papilloma.[J].Histol Histopathol,2013,28:53-60.
[7]	WOOLDRIDGE T R,LAIMINS L A.Regulation of human papillomavirus type 31gene expression during the differentiation-dependent life cycle through histone modifications and transcription factor binding[J].Virology,2008,374:371-380.
[8]	LAWSON W,SCHLECHT N F,BRANDWEINGENSLER M.The role of the human papillomavirus in the pathogenesis of Schneiderian inverted papillomas:an analytic overview of the evidence[J].Head Neck Pathol,2008,2:49-59.
[9]	DOOHER J E,PAZ-PRIEL I,HOUNG S,et al.C/EBP alpha,C/EBP alpha oncoproteins,or C/EBP beta preferentially bind NF-kappa B p50 compared with p65,focusing therapeutic targeting on the C/EBP:p50 interaction[J].Molecular Cancer Res,2011,9:1395-1405.
[10]	BENNETT K L.Activator protein 2α(AP2α)suppresses 42kDa C/CAAT enhancer binding proteinα(p42C/EBPα)in head and neck squamous cell carcinoma(HNSCC)[J].Int J Cancer,2009,124:1285-1292.
[11]	GRANDINETTI K B,STEVENS T A,HA S,et al.Overexpression of TRIB2 in human lung cancers contributes to tumorigenesis through downregulation of C/EBPα[J].Oncogene,2011,30:3328-3335.
[12]	肖艳景,张全武,娄欣,等.CK10、CK14、CK17、P16在宫颈不成熟鳞状化生及宫颈上皮内瘤变中的表达[J].医药论坛杂志,2010,31(2):132-134.
[13]	CARLA C,MATOS A,LANDIM B.Keratin 8,10,13and 17are useful markers in the diagnosis of human cervix carcinomas[J].Hum Pathol,2004,35:546-551.
[14]	王元元,伍津津.人原代表皮干细胞及其传代细胞生物学特性的比较[J].第三军医大学学报,2011,33(1):24-27.