鼻内翻性乳头状瘤中COX-2、p21、Ki-67的表达及HPV感染的临床意义

孟宪影; 邬旭; 袁益兵

doi:10.13201/j.issn.1001-1781.2014.23.002

鼻内翻性乳头状瘤中COX-2、p21、Ki-67的表达及HPV感染的临床意义

- 大连医科大学附属第二医院耳鼻咽喉科(辽宁大连, 116027)
基金项目:
辽宁省自然科学基金(No:20102051)

详细信息

通讯作者: 邬旭,E-mail:wu-xu2008@sohu.com

中图分类号: R730.261

收稿日期: 2014-04-23

Significances of COX-2,p21,Ki-67expression and HPV infection in nasal inverted papilloma

- Department of Otolaryngology, the Second Affiliated Hospital of Dalian Medical University, Dalian, 116027, China

More Information

Corresponding author: WU Xu, E-mail: wu-xu2008@sohu.com

Received Date: 23 April 2014

摘要

摘要: 目的:探讨鼻内翻性乳头状瘤组织中COX-2、p21、Ki-67的表达及HPV感染的临床意义。方法:应用免疫组织化学二步法检测30例鼻内翻性乳头状瘤(NIP)和20例鼻息肉(NP)及10例正常鼻腔黏膜组织(NM)中COX-2、p21、Ki-67表达情况,并用导流杂交方法检测HPV感染的情况。结果:NIP组、NP组和NM组中COX-2、Ki-67的阳性表达率呈依次降低的趋势;COX-2在3组间比较差异有统计学意义(χ²=30.00,P<0.05);Ki-67在NIP和NM组间比较差异有统计学意义(χ²=8.533,P<0.05)。经Spearman秩相关分析,COX-2与Ki-67的表达呈正相关(r=0.78,P<0.05)。p21在NIP组中未见表达。NIP组HPV病毒检出率为26.67%,均为HPV16型。结论:COX-2、Ki-67和HPV感染与NIP的发生、发展有一定相关性。COX-2介导的炎症反应,可能作为NIP发病的危险因素。Ki-67能较好地反映肿瘤细胞增殖活性,可作为衡量鼻内翻性乳头状瘤的增殖活性的指标。COX-2与Ki-67的表达在NIP的发病中具有协同作用。p21与NIP发病无明显相关。HPV感染与NIP的发病有关,但不能作为NIP发病的主要因素。
- 乳头状瘤 /
- 内翻 /
- 环氧化酶-2 /
- p21 /
- Ki-67 /
- 人乳头状瘤病毒 /
- 免疫组织化学
Abstract: Objective:To investigate the significance of expression of COX-2,p21, Ki67 and HPV in nasal inverted papilloma. Method:Detecting COX-2, p21, Ki-67 in 30 cases of nasal inverted papilloma (NIP), 20 cases of nasal polyps (NP) and 10 cases of normal nasal mucosa (NM) by two step immunohistochemical method, and HPV virus by flow-through hybridization method.Result:The positive expression rate of COX-2 and Ki-67 in NIP, NP and NM group was decreased in turn, COX-2 had significant difference in the groups(χ²=30.00, P<0.05); the positive expression rate of Ki-67 had significant differences between NIP and NM group (χ²=8.533, P<0.05). The expression of COX-2 in NIP tissues was positively correlate with that of Ki-67 by using Spearman rank correlation analysis (r=0.78, P<0.05). Expression of p21 were not observed in NIP group. The positive rate of HPV was 26.67% in 30 cases of NIP, all of HPV16 type. Conclusion:COX-2, Ki-67 and HPV infection have certain correlation with the occurrence of NIP. The occurrence of NIP has relationship with inflammatory reaction mediated by COX-2. Ki-67 can well reflect the proliferation activity of tumor cells, and can be used to measure the proliferation rate of nasal inverted papilloma. The COX-2 and Ki-67 have a synergistic role in the pathogenesis of NIP. p21 has no significant relationship with the incidence of NIP. HPV infection is related to the pathogenesis of NIP, but not as a major factor in the pathogenesis of NIP.
- papilloma inverted /
- COX-2 /
- p21 /
- Ki-67 /
- human papilloma virus /
- immunohistochemistry /

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