Msx2、topoⅡ-α、HPV16及VEGF在鼻内翻性乳头状瘤中的定量及意义

张峻峥; 杨一兵; 汤勇; 吴锡芳; 丛林海; 阮标

doi:10.13201/j.issn.1001-1781.2014.23.001

Msx2、topoⅡ-α、HPV16及VEGF在鼻内翻性乳头状瘤中的定量及意义

- 昆明医科大学第一附属医院耳鼻咽喉科(昆明, 650032)
基金项目:
云南省应用基础研究面上项目(No:2010ZC124)

详细信息

通讯作者: 杨一兵,E-mail:369300770@qq.com

中图分类号: R730.261

收稿日期: 2013-10-27

The quantification and significance of muscle segment homeobox gene Msx2,human topoisomerase Ⅱ-α,HPV 16 and VEGF in sinonasal inverted papilloma

- Department of Otolaryngology, the First Affiliated Hospital of Kunming Medical College, Kunming, 650032, China

More Information

Corresponding author: YANG Yibing, E-mail: 369300770@qq.com

Received Date: 27 October 2013

摘要

摘要: 目的:探讨Msx2、topoⅡ-α、HPV16及VEGF在鼻内翻性乳头状瘤(SNIP)组织中的定量及意义,研究上述四种因子之间是否具有相关性,进一步确定Msx2和topoⅡ-α在SNIP恶变发生、发展中的关系。方法:采用实时荧光定量聚合酶链反应(RT-qPCR)分别检测Msx2、topoⅡ-α、VEGF及HPV16在13例SNIP,10例鼻息肉(INP),10例鼻腔-鼻窦鳞状细胞癌组织(NSCC)中的表达。其中SNIP根据病理形态分为轻度不典型增生组、中度不典型增生组和重度不典型增生组。结果:SNIP和NSCC组织中Msx2、topoⅡ-α、HPV16及VEGF的表达均显著高于INP(P<0.05)。Msx2、topoⅡ-α、VEGF及HPV16在SNIP的3个病理形态组之间的表达差异均有统计学意义(P<0.05)。采用Pearson相关系数得出HPV16、topoⅡ-α、VEGF、Msx2之间两两比较均呈正相关性(P<0.05)。结论:Msx2和topoⅡ-α在SNIP恶变的发生、发展中起重要作用,有可能成为SNIP和NSCC基因治疗的新靶点。
- 乳头状瘤 /
- 内翻 /
- 肌节同源盒基因2 /
- 人拓扑异构酶Ⅱ-α /
- 人乳头状瘤病毒16 /
- 血管内皮生长因子
Abstract: Objective:To investigate the quantification and significance of Msx2, topoⅡ-α, HPV16 and VEGF in sinonasal inverted papilloma(SNIP),to study the correlation among the four factors,and to discover the relationship between Msx2 and topoⅡ-α in the process of SNIP malignant transfomation.Method:Real-time quantitative Polymerase Chain Reaction (RT-qPCR) was used to detect the expression of Msx2, topoⅡ-α, HPV16 and VEGF in 13 cases of sinonasal inverted papilloma (SNIP),10 cases of sinonasal squamous cell carcinoma(NSCC) and 10 cases of inflammatory nasal polyp paraffin (INP)tissues. According to the pathology results SNIP were divided into mild dysplasia, moderate dysplasia and severe dysplasia. All the data were analysised by SPSS17.0,P<0.05 was refered to statistically significant difference.Result:The mRNA level of Msx2, topoⅡ-α, VEGF and HPV16 in SNIP, NSCC tissues were significantly higher than in the INP tissues (P<0.05). The expression differences of Msx2, topoⅡ-α, HPV16 and VEGF mRNA level in SNIP tissues which were divided into three groups according to their pathological results,were all statistically significantly different between any two of the three groups (P<0.05). Using Pearson correlation coefficient analysis,we found positive correlation between any two of the mRNA level of Msx2, topoⅡ-α, VEGF and HPV16 (P<0.05).Conclusion:Msx2 and topoⅡ-α may play an important role in the process of SNIP Malignant transformation,which may be new targets for gene therapy of SNIP and NSCC.
- papilloma inverted /
- Msx2 /
- topoⅡ-α /
- HPV16 /
- VEGF /

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