miRNA-324-3p及其靶基因WNT2B在鼻咽癌组织中的表达及临床意义

刘超; 李果; 刘勇; 粟忠武; 任舒灵; 邓腾波; 田勇泉; 邱元正

doi:10.13201/j.issn.1001-1781.2014.21.013

miRNA-324-3p及其靶基因WNT2B在鼻咽癌组织中的表达及临床意义

- 中南大学湘雅医院耳鼻咽喉头颈外科耳鼻咽喉重大疾病研究湖南省重点实验室(长沙, 410008)
基金项目:
国家自然科学基金(No:81372426,No:81202128,No:81172558)

高等学校博士学科点专项科研基金课题(No:20120162120049)

中南大学自由探索计划青年教师助推基金(No:2012QNZT099)

湖南省博士生创新项目基金(No:CX2013B108)

中南大学贵重仪器设备开放共享基金(No:CSUZC2014048)

详细信息

通讯作者: 邱元正,E-mail:xyqyz@hotmail.com

中图分类号: R739.6

收稿日期: 2014-06-18

The expression and significance of miRNA-324-3p and WNT2B in nasopharyngeal carcinoma

- Department of Otorhinolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University, Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008, China

More Information

Corresponding author: QIU Yuanzheng, E-mail: xyqyz@hotmail.com

Received Date: 18 June 2014

摘要

摘要: 目的:探讨miRNA-324-3p及其靶基因WNT2B在鼻咽癌组织中的表达和临床意义。 方法:qRT-PCR技术检测miRNA-324-3p、WNT2B mRNA的相对表达量,Western blot技术检测WNT2B蛋白在39例鼻咽癌组织及21例鼻咽非肿瘤上皮组织中的表达,分析其表达水平与鼻咽癌临床病理特征的关系。结果:在鼻咽癌组织中miRNA-324-3p的相对表达量显著下调(P<0.01),WNT2B mRNA和蛋白的相对表达量则显著上调(均P<0.01)。鼻咽癌组织中miRNA-324-3p与WNT2B mRNA及蛋白的表达呈负相关,WNT2B mRNA与蛋白的表达呈正相关(均P<0.05)。miRNA-324-3p、WNT2B mRNA及蛋白的表达均与鼻咽癌患者T分级、临床分期及淋巴结转移密切相关(均P<0.05),而与患者的性别、年龄无明显相关性(均P>0.05)。结论:鼻咽癌组织中miRNA-324-3p的表达下调,WNT2B 的表达上调,两者可能存在相互关系,共同介导鼻咽癌的发生、发展。
- 鼻咽肿瘤 /
- miRNA-324-3p /
- WNT2B /
- 基因
Abstract: Objective:To investigate the expression and significance of miRNA-324-3p and its target gene WNT2B in tissue specimens of nasopharyngeal carcinoma (NPC) specimens. Method:qRT-PCR was used to detect the expression of miRNA-324-3p and WNT2B mRNA, and Western blot was applied to assay the expression of WNT2B protein in 39 cases of NPC specimens and 21 cases of non-carcinoma epithelium. The relationship between their expression levels and clinicopathological characteristics and their correlation with clinical pathological parameters was analyzed. Result:The expression of miRNA-324-3p was significantly down-regulateddecreased but WNT2B mRNA/protein increased obviously in NPC specimens(P<0.01). A negative correlation between miRNA-324-3p and WNT2B was spotted(P<0.05). The expression levels of these markers were closely correlated with T stage, clinic stage and cervical lymph node metastasis (P<0.05). Conclusion:The loss of miRNA-324-3p and ectopic WNT2B might co-induce the initiation and progression of NPC.
- nasopharyngeal carcinoma /
- miRNA-324-3p /
- WNT2B /
- gene

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