大蒜油联合5-FU诱导腺样囊性癌细胞株ACC-M凋亡的研究

吴发印; 周鹤峰; 樊治英; 朱雅文; 李永烨; 姚裕坤; 冉丹

doi:10.13201/j.issn.1001-1781.2014.03.009

大蒜油联合5-FU诱导腺样囊性癌细胞株ACC-M凋亡的研究

- 1 遵义医学院第五附属(珠海)医院口腔科(广东珠海, 519100);
- 2 遵义医学院珠海校区生物系;
- 3 遵义医学院珠海校区口腔系
基金项目:
贵州省科学技术基金项目[No:黔科合J字LKZ (2010) 07号]

遵义医学院大学生创新性实验计划项目 (No:D-12-11)

详细信息

通讯作者: 吴发印, E-mail:wfy660505@sina.com

中图分类号: R730.261

收稿日期: 2013-03-20

Study on garlic oil combined with 5-FU induced apoptosis of adenoid cystic carcinoma cell line ACC-M

- 1 Department of Stomatology, the Fifth Affiliated Hospital, Zunyi Medical College, Zhuhai, 519100, China;
- 2 Department of Biotechnology, Zunyi Medical College Zhuhai Campus;
- 3 Department of Stomatology, Zunyi Medical College Zhuhai Campus

More Information

Corresponding author: WU Fayin, E-mail: wfy660505@sina.com

Received Date: 20 March 2013

摘要

摘要: 目的:探讨大蒜油联合5-FU诱导腺样囊性癌细胞株ACC-M凋亡的效应。方法:人涎腺腺样囊性癌细胞株ACC-M细胞培养,分为实验组(5-FU组、大蒜油组、大蒜油+5-FU组)和对照组,采用MTT法观察肿瘤细胞的体外生长活性;采用流式细胞术分析肿瘤细胞周期和凋亡率的变化。结果:MTT实验结果显示不同浓度的5-FU、大蒜油、大蒜油+5-FU对ACC-M细胞均有抑制作用,随着浓度和作用时间的增加而呈上升趋势;流式细胞术分析显示细胞周期发生明显变化,随着浓度增加、作用时间延长G₀/G₁期的细胞比率上升,S期无明显变化,而G₂/M期的细胞下降,凋亡率显示大蒜油联合5-FU诱导ACC-M细胞凋亡明显强于单独组。结论:大蒜油能有效诱导腺样囊性癌细胞株ACC-M凋亡,大蒜油联合5-FU应用对ACC-M细胞的作用明显强于大蒜油,5-FU可单独应用。
- 大蒜油 /
- 5-FU /
- 癌 /
- 腺样囊性 /
- 凋亡
Abstract: Objective:To Observe the effect of garlic oil combined with 5-FU induced apoptosis of adenoid cystic carcinoma cell line ACC-M.Method:Human salivary adenoid cystic carcinoma cell line ACC-M was cultured, divided into the experimental group(5-FU group, garlic oil group, garlic oil+5-FU group) and the control group, to observe the growth activity of tumor cells by MTT methods;to analyse the changes of cell cycle and apoptosis rate by flow cytometry.Result:MTT experiments showed that 5-FU,garlic oil,garlic oil and +5-FU on ACC-M cells have inhibition in different concentration, with the increase of concentration and action time of the rise; Cell cycle analysis showed significant changes in flow cytometry. With the increase of concentration and the acting time, the G₀/G₁ phase of the cell ratio increased, S had no significant change, but G₂/M phase cells decreased. Apoptosis rate display showed garlic oil combined with 5-FU induced apoptosis of ACC-M cells was significantly stronger than single group.Conclusion:Garlic oil can effectively induce the apoptosis of adenoid cystic carcinoma cell line ACC-M.The effect of garlic oil combined with 5-FU on ACC-M cells was stronger than the garlic oil, 5-FU used alone.
- garlic oil /
- 5-FU /
- carcinoma /
- adenoid cystic /
- apoptosis

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参考文献(11)

[1]	张志愿.口腔颌面外科学[M].7版.北京:人民卫生出版社, 2012:369-370.
[2]	谭向荣, 葛明华.头颈部腺样囊性癌的治疗进展[J].中国肿瘤, 2011, 20 (11):847-849.
[3]	王学辰.腮腺腺样囊性癌的治疗进展[J].中国癌症杂志, 2008, 18 (8):710-713.
[4]	张翠丽, 曾涛, 赵秀兰, 等.大蒜油与顺铂对人卵巢癌SKOV_3细胞生长抑制的联合作用[J].毒理学杂志, 2010, 24 (2):116-119.
[5]	RAJPUT S, MANDAL M.Antitumor promoting potential of selected phytochemicals derived from spices:a review[J].Eur J Cancer Prev, 2012, 21:205-215.
[6]	ICIEK M, KWIECIEN I, WLODEK L.Biological properties of garlic and garlic-derived organosulfur compounds[J].Environ Mol Mutagen, 2009, 50:247-265.
[7]	ZHANG W, HA M, GONG Y, et al.Allicin induces apoptosis in gastric cancer cells through activation of both extrinsic and intrinsic pathways[J].Oncol Rep, 2010, 24:1585-1592.
[8]	BAT-CHEN W, GOLAN T, PERI I, et al.Allicin purified from fresh garlic cloves induces apoptosis in colon cancer cells via Nrf2[J].Nutr Cancer, 2010, 62:947-957.
[9]	TSUBURA A, LAI Y C, KUWATA M, et al.Anticancer effects of garlic and garlic-derived compounds for breast cancer control[J].Anticancer Agents Med Chem, 2011, 11:249-253.
[10]	PADILLA-CAMBEROS E, ZAITSEVA G, PADILLA C, et al.Antitumoral activity of allicin in murine lymphoma L5178Y[J].Asian Pac J Cancer Prev, 2010, 11:1241-1244.
[11]	吴发印, 陈琼, 周鹤峰, 等.大蒜素对黏液表皮样癌细胞株抑制作用的研究[J].临床耳鼻咽喉头颈外科杂志, 2010, 24 (21):987-989.