喉癌组织中IGFBP-rP1基因的甲基化及表达

胡俊兰; 赵瑞力; 吴干勋; 王晶田

doi:10.13201/j.issn.1001-1781.2013.24.007

喉癌组织中IGFBP-rP1基因的甲基化及表达

- 河北医科大学第四医院耳鼻咽喉头颈外科(石家庄, 050011)
基金项目:
河北省科技计划项目 (No:122777117)

详细信息

通讯作者: 胡俊兰,E-mail:wangjingtian9985@sina.com

中图分类号: R739.65

收稿日期: 2013-02-18

Expression and methylation status of IGFBP-rP1 gene in laryngocarcinoma

- Department of Otolaryngology Head and Neck Surgery, the Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, China

More Information

Corresponding author: HU Junlan, E-mail: wangjingtian9985@sina.com

Received Date: 18 February 2013

摘要

摘要: 目的:探讨喉癌中胰岛素样生长因子结合蛋白-相关蛋白1 (IGFBP-rP1)基因启动子区甲基化与蛋白表达之间的关系。方法:采用甲基化特异性PCR方法和免疫组织化学的方法分别检测45例喉癌组织及18例癌旁组织中IGFBP-rP1基因的甲基化状态和蛋白表达情况。结果:喉癌组织IGFBP-rP1启动子区甲基化率为33.3%(15/45),相应癌旁组织甲基化率为5.6%(1/18),喉癌组织中IGFBP-rP1基因甲基化率明显高于癌旁组织(P<0.05)。喉癌组织中IGFBP-rP1蛋白表达显著低于癌旁正常组织(P<0.05),且与其启动子区甲基化状态呈负相关。结论:IGFBP-rP1基因启动子区甲基化导致基因沉默可能是喉癌发生的机制之一。
- 喉肿瘤 /
- DNA甲基化 /
- 胰岛素样生长因子结合蛋白-相关蛋白1 /
- 蛋白表达
Abstract: Objective: To investigate the relationship between the promoter methylation and protein expression of insulin-like growth factor binding protein-related protein 1(IGFBP-rP1) in laryngeal squamous cell carcinoma(LSCC).Method: Methylation specific PCR (MSP) approach and immunohistochchemistry methods were used to examine the methylation status and protein expression of IGFBP-rP1 in 45 samples of laryngeal carcinoma and 18 samples of tissues beside cancer.Result: For the promoter site, methylation frequency of IGFBP-rP1 in tumor specimens(33.3%, 15/45) was significantly higher than that in corresponding nomal tissues(5.6%, 1/18)(P<0.05). The protein expression of IGFBP-rP1 in tumor tissues was significantly higher than that in corresponding normal tissues(P<0.05) and was inversely correlated with its methylation status of promoter.Conclusion: Epigenetic silencing of IGFBP-rP1 gene expression by promoter hypermethylation may play an important role in LSCC.
- laryngeal neoplasms /
- DNA methylation /
- IGFBP-rP1 gene /
- protein expression

HTML全文

参考文献(8)

[1]	ZUO S, LIU C, WANG J, et al. IGFBP-rP1 induces p21 expression through a p53-independent pathway,leading to cellular senescence of MCF-7 breast cancer cells[J]. Cancer Res Clin Oncol, 2012, 138:1045-1055.
[2]	LIN J, LAI M, HUANG Q, et al. Reactivation of IGFBP7 by DNA demethylation inhibits human colon cancer cell growth in vitro[J]. Cancer Biol Ther, 2008,7:1896-1900.
[3]	CHEN Y, PACYNA-GENQELBACH M, YE F, et al. Insulin-like growth factor binding protein-related protein 1(IGFBP-rP1) has potential tumor- suppressive activity in human lung cancer[J]. J Pathol, 2007, 211:431- 438.
[4]	BIRD A. Molecular biology. Methylation talk between histones and DNA[J]. Science, 2001, 294:2113-2115.
[5]	SMITH P, NICHOLSON LJ, SYED N, et al. Epigenetic inactivation implies independent functions for insulin-like growth factor binding protein(IGFBP)-related protein 1 and the related IGFBPL1 in inhibiting breast cancer phenotypes[J]. Clin Cancer Res,2007,13:4061-4068.
[6]	YAMASHITA S, TSUJINO Y, MORIGUCHI K, et al. Chemical genomic screening for methylation-silenced genes in gastric cancer cell lines using 5-aza-2'-deoxycytidine treatment and oligonucleotide microarray[J]. Cancer Sci, 2006, 97:64-71.
[7]	MCCAIG C, PERKS C M, HOLLY J M. Intrinsic actions of IGFBP-3 and IGFBP-5 on Hs578T breast cancer epithelial cells:inhibition or accentuation of attachment and survival is dependent upon the presence of fibronectin[J]. J Cell Sci, 2002, 115:4293-4303.
[8]	DEGEORGES A, WANG F, FRIERSON H F Jr., et al. Human prostate cancer expresses the low affinity insulin-like growth factor binding protein IGFBP-rP1[J]. Cancer Res, 1999, 59:2787-2790.