磷脂酶参与的花生四烯酸12-LOX代谢通路在喉鳞状细胞癌中的作用及临床意义

王嘉; 李晓明; 徐鸥; 单春光

doi:10.13201/j.issn.1001-1781.2013.24.008

磷脂酶参与的花生四烯酸12-LOX代谢通路在喉鳞状细胞癌中的作用及临床意义

- 1 河北医科大学第二医院耳鼻咽喉科(石家庄, 050000);
- 2 白求恩国际和平医院耳鼻咽喉科

详细信息

通讯作者: 单春光,E-mail:shanchunguang@163.com

中图分类号: R739.65

收稿日期: 2013-01-30

The role and clinical significance of 12-LOX passway in arachidonic acid metabolism induced by phospholipase Cγ-2 in laryngeal squamous cell carcinoma

- 1 Department of Otolaryngology, the Second Affiliated Hospital of Hebei Medical University, Shijiazhuang, 050000, China;
- 2 Department of Otolaryngology, the Peace Hospital of Hebei Medical University

More Information

Corresponding author: SHAN Chunguang, E-mail: shanchunguang@163.com

Received Date: 30 January 2013

摘要

摘要: 目的:研究磷脂酶(PL)Cγ-2、花生四烯酸(AA)以及脂加氧酶-12(12-LOX)在喉鳞状细胞癌中的表达情况,探讨脂质代谢与喉鳞状细胞癌的关系。方法:选取30例喉癌组织以及癌旁组织(均经病理检查为正常组织),以免疫组织化学法(SP法)检测PLCγ-2和12-LOX的表达,以气相色谱联合质谱法检测AA的含量。结果:喉癌组织中PLCγ-2和12-LOX的表达高于正常组织(P<0.05),而AA含量低于正常组织(P<0.05);PLCγ-2和12-LOX与喉癌的临床分期、病理分化以及淋巴结转移有密切关系(P<0.05);AA与喉癌的临床分期、病理分化以及淋巴结转移无关(P＞0.05);PLCγ-2、AA以及12-LOX与年龄无关(P＞0.05)。结论:PLCγ-2、AA和12-LOX三者与喉癌关系密切,阻断此通路,可能对喉癌的治疗有一定的意义。
- 喉肿瘤 /
- 磷脂酶Cγ-2 /
- 脂加氧酶-12 /
- 花生四烯酸
Abstract: Objective: To explore the expression of phospholipase Cγ-2(PLCγ-2), lipoxygenase-12(12-LOX) and arachidonic acid (AA) in laryngeal squamous cell carcinoma and to study the the relationship between lipid metabolism and laryngeal squamous cell carcinoma.Method: In 30 cases of carcinoma tissue and peritumoral laryngeal mucosa tissues(confirmed to be normal laryngeal tissues by pathology), immunohistochemical method (Streptavidin-peroxidase method,SP method) was used for the detction of expression of PLCγ-2 and 12-LOX,and gas chromatography/mass spectrometry(GC/MS) for the content of the arachidonic acid in carcinoma tissue and peritumoral normal laryngeal mucosa tissues.Result: The positive rates of PLCγ-2 and 12-LOX in carcinoma tissue were higher than in peritumoral normal laryngeal mucosa tissues with statistically significance differences (P<0.05).The content of arachidonic acid was lower in carcinoma tissue than in peritumoral normal laryngeal mucosa tissues with statistically significance difference(P<0.05).The positive expressions of PLCγ-2 and 12-LOX were closely correlated to tnm stage, histological differentiation and lymph node metastasis(P<0.05).The content of arachidonic acid had no significant correlations with tnm stage, histological differentiation and lymph node metastasis(P＞0.05). Both the expression of PLCγ-2 and 12-LOX and the content of arachidonic acid had no statistically significant correlation with age(P＞0.05).Conclusion: PLCγ-2,AA and 12-LOX play important roles in laryngeal squamous cell carcinoma. It may be meaningful to the treatment of laryngeal carcinoma by suppressing this passway.
- larygeal neoplasms /
- phospholipase Cγ-2 /
- lipoxygenase-12 /
- arachidonic acid

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