Changes of extracellular matrix of nasal mucosa in guinea pig model of prolonged allergic-induced rhinitis
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摘要: 目的: 观察抗原长期刺激变应性鼻炎豚鼠模型鼻黏膜细胞外基质(ECM)变化的特征。方法: Hartley豚鼠32只,随机分为4组(每组8只),即实验2 w组、实验6 w组、实验12 w组和对照组。卵清蛋白制备变应性鼻炎动物模型,抗原刺激分别持续2 w、6 w和12 w。在最后一次激发24 h后处死动物取鼻黏膜组织。鼻黏膜组织切片,采用苏木精-伊红染色计数嗜酸粒细胞数量、测量鼻黏膜上皮厚度;三色胶原染色法染色测定ECM胶原蓝染面积百分比;免疫组织化学法检测鼻黏膜组织转移生长因子-β1(TGF-β1)及Ⅰ、Ⅲ型胶原的吸收度。结果: ①各实验组鼻黏膜均有大量嗜酸粒细胞浸润,上皮增厚,与对照组比较差异有统计学意义。②实验2 w组、实验6 w组、实验12 w组和对照组鼻黏膜ECM胶原蓝染面积百分比分别为(18.6±12.0)%、(22.9±9.3)%、(21.7±1.3)%和(15.6±4.0)%;实验6 w组、实验12 w组ECM胶原蓝染面积百分比与对照组比较均差异有统计学意义(均P<0.01),但实验2 w组与对照组比较差异无统计学意义。③同对照组比较,各实验组TGF-β1吸收度增加,差异有统计学意义;Ⅰ、Ⅲ型胶原吸收度在各实验组都呈增加趋势。结论: 随着抗原刺激延长,鼻黏膜慢性炎症导致与重塑相关的炎性因子表达增强,ECM胶原沉积明显。Abstract: Objective: The aim of this study was to explore the changes of the extracellular matrix in nasal mucosa by a guinea pig model of prolonged allergic-induced rhinitis.Method: Thirty-two male Hartley guinea pigs were randomly divided into four groups: allergen challenged groups(Group 2 w,Group 6 w and Group 12 w) and a control group.Ovalbumin-sensitized guinea pigs were repeatedly challenged with allergen twice a week from 2 weeks to 12 weeks. Matched control groups were challenged with physiological saline. Nasal mucosa were obtained from the animals killed. Hematoxylin-Eosin, Masson's trichrome, and immunohistochemical staining against transforming growth factor-β1(TGF-β1),Collagen Ⅲ and CollagenⅠwere performed to nasal mucosa.Result: ①Pathological examination showed obvious infiltration of eosinophils and the enlarged thickness of epithelial layer of nasal mucosa in the experiment groups.②The area ratios of blue stained in the extracellular matrix of nasal mucosa were increased. The area ratios of blue stained were statistically different in Group 6 w and Group 12 w compared with the control group.③The increasing absorbance of TGF-β1 were statistically different in the experiment groups with the control group. The absorbance of Collagen Ⅲ and CollagenⅠshowed a rising trend along prolonged allergen challenged in the experiment groups.Conclusion: Prolonged allergen challenge and the inflammation of nasal mucosa, can lead to the increasing of the inflammation relevant factors and the deposit of collagen in the extracellular matrix of nasal mucosa.
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Key words:
- rhinitis /
- allergic /
- extracellular matrix /
- collagen /
- transforming growth factor-β1
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