CD44调控鼻咽癌上皮-间质转化及转移的实验研究

王爽; 李仕晟; 谢鼎华; 唐青来; 王树辉; 刘家佳; 陈月红; 杨新明

doi:10.13201/j.issn.1001-1781.2013.05.010

CD44调控鼻咽癌上皮-间质转化及转移的实验研究

- 中南大学湘雅二医院耳鼻咽喉头颈外科 (长沙, 410011)

详细信息

通讯作者: 杨新明,E-mail:x16y2003@yahoo.com.cn

中图分类号: R739.6

收稿日期: 2012-04-10

CD44 regulates epithelial-mesenchymal transition and metastasis in nasopharyngeal cancer cells

- Department of Otolaryngology-Head and Neck Surgery, the Second Xiangya Hospital, Central South University, Changsha, 410011, China

More Information

Corresponding author: YANG Xinming, E-mail: x16y2003@yahoo.com.cn

Received Date: 10 April 2012

摘要

摘要: 目的: 研究鼻咽癌细胞中CD44与上皮-间质转化(EMT)及转移的相关性,并初步探讨CD44调控鼻咽癌细胞EMT及转移的机制。方法: Western blotting检测鼻咽癌细胞株5-8F和6-10B中CD44与EMT相关因子的表达。用带有CD44基因的质粒以脂质体瞬时转染鼻咽癌细胞株6-10B获得CD44高表达的鼻咽癌细胞。Western blotting检测转染前后细胞中CD44与EMT相关因子的表达。细胞划痕实验检测6-10B转染前后细胞迁移能力的改变。结果: 鼻咽癌细胞株5-8F中CD44和EMT相关因子基质金属蛋白酶-9(MMP-9)的表达量高于6-10B,而EMT相关因子E-钙黏蛋白的表达量低于6-10B,5-8F的迁移能力强于6-10B。CD44基因转染后的6-10B中CD44及MMP-9的表达均升高。CD44基因转染后的6-10B迁移能力明显增强。结论: 在鼻咽癌细胞中,CD44通过调控EMT进程影响鼻咽癌细胞的转移能力。
- CD44 /
- 上皮-间质转化 /
- 基质金属蛋白酶-9 /
- 鼻咽肿瘤 /
- 转移
Abstract: Objective: To study the correlation of CD44 with epithelial-mesenchymal transition(EMT) and metastasis in nasopharyngeal cancer cells, and explore the possible mechanism of CD44 regulates EMT and metastasis in nasopharyngeal cancer cells.Method: The CD44 and EMT-associated proteins in 5-8F and 6-10B nasopharyngeal cancer cell lines were assayed by Western blotting. The erasion trace test was performed to observe the migratory ability of 5-8F and 6-10B nasopharyngeal cancer cells. Using lipid-mediated DNA transfection technique, the low metastatic nasopharyngeal cancer cells 6-10B were transfected in vitro with plasmid which contained CD44 gene, and then new nasopharyngeal cancer cells were obtained. The CD44 and EMT-associated proteins in 6-10B, empty vector transfected and CD44-transfected cells were assayed by Western blotting. The erasion trace test was performed to observe the alteration of migratory ability of nasopharyngeal cancer cells before and after CD44 transfection.Result: The expression of CD44 and EMT-associated protein MMP-9 in 5-8F was higher than that in 6-10B, but EMT-associated protein E-Cadherin in 5-8F was lower than that in 6-10B. The migratory ability of 5-8F was higher than that of 6-10B. The expression of CD44 and MMP-9 were significantly higher in the CD44-transfected nasopharyngeal cancer cells than in the control groups. Compared with control groups, the migratory ability of CD44-transfected nasopharyngeal cancer cells was significantly increased.Conclusion: CD44 positively regulates the metastatic ability of nasopharyngeal cancer cells, which is relevant to the process of EMT.
- CD44 /
- epithelial-mesenchymal transition /
- matrix metalloproteinase-9 /
- nasopharyngeal carcionma /
- metastasis

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