Differential expression of NEDD8 in different pathological types of chronic rhinosinusitis with nasal polyps
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摘要: 目的 分析不同病理类型的慢性鼻窦炎伴鼻息肉(chronic rhinosinusitis with nasal polyps，CRSwNP)患者的鼻息肉组织中神经前体细胞表达发育下调蛋白8(Neural precursor cell-expressed developmentally downregulated 8，NEDD8)表达水平差异。方法 使用在北京同仁医院耳鼻咽喉头颈外科因慢性鼻窦炎行鼻内镜手术的患者标本。利用苏木精-伊红染色检测鼻息肉组织中的嗜酸性粒细胞数量，并根据鼻息肉组织中嗜酸性粒细胞的数量对CRSwNP患者进行分组，采用免疫组织化学法检测分析鼻息肉组织中的NEDD8蛋白表达水平。结果 嗜酸性粒细胞型慢性鼻窦炎伴鼻息肉患者鼻息肉中NEDD8蛋白表达水平显著高于非嗜酸性粒细胞型慢性鼻窦炎伴鼻息肉患者(P<0.05)。NEDD8蛋白表达水平和鼻息肉组织中嗜酸性粒细胞数量呈显著正相关(r=0.79，P=0.02)。结论 不同病理类型的CRSwNP患者NEDD8蛋白表达存在差异。
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关键词:
- 神经前体细胞表达发育下调蛋白8 /
- 鼻窦炎 /
- 鼻息肉 /
- 嗜酸性粒细胞
Abstract: Objective To analyze the differential expression of neural precursor cell-expressed developmentally downregulated 8(NEDD8) protein in nasal polyp tissues of patients with different pathological types of chronic rhinorhinosinusitis with nasal polyps(CRSwNP).Methods All specimens were obtained from the specimen library of Beijing Tongren Hospital, and were all patients who underwent nasal endoscopic surgery for chronic rhinosinusitis in Beijing Tongren Hospital. Hematoxylin-eosin staining(HE) was used to detect the number of eosinophils in nasal polyps, and CRSwNP patients were grouped according to the number of eosinophils in nasal polyps, immunohistochemistry was used to detect and analyze the expression level of NEDD8 protein in nasal polyps.Results The expression level of NEDD8 protein in nasal polyps of patients with eosinophilic chronic rhinorhinosinusitis with nasal polyps was significantly higher than that of patients with non-eosinophilic chronic rhinosinusitis and nasal polyps(P<0.05). In addition, there was a significant positive correlation between the expression level of NEDD8 protein and the number of eosinophils in nasal polyp tissue(r=0.79, P=0.02).Conclusion There are differences in the expression of NEDD8 protein in patients with chronic rhinosinusitis and nasal polyps of different pathological types. -
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