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摘要: 目的 测量大连地区50例健康人经鼻呼出气一氧化氮(nNO)浓度值, 通过研究安静状态下改变鼻腔不通气时间时NO浓度的变化趋势, 为无创检测鼻窦内NO浓度及鼻窦口通畅性提供思路。方法 ① 使用纳库仑呼气分析仪检测大连地区50例健康受试者的左、右侧nNO浓度值(LnNO、RnNO)及设定鼻腔不通气时间分别为10 s、20 s、30 s时经左侧鼻腔测得的LnNO10、LnNO20、LnNO30, 对其中配合良好的10例受试者延长鼻腔不通气时间至50 s, 测得LnNO50。②使用SPSS 19.0进行统计学分析, 采用t检验分析双侧nNO之间的差异, 通过单因素重复测量方差分析研究改变鼻腔不通气时间时NO在鼻腔的蓄积浓度变化趋势。结果 ① 50例健康受试者LnNO浓度平均值为(383.32±126.35)×10-9, RnNO平均值为(395.26±124.32)×10-9, 双侧nNO浓度之间差异无统计学意义(P>0.05), 平均值为(389.29±124.85)×10-9。②鼻腔不通气时间为10、20、30 s时测得nNO浓度平均值LnNO10、LnNO20、LnNO30分别为(984.54±477.69)×10-9、(1527.32±717.25)×10-9和(2183.26±946.21)×10-9, 其中10例受试者鼻腔不通气时间延长至50 s时测得LnNO50为(3083.00±1905.62)×10-9; 延长鼻腔不通气时间后测得nNO浓度升高的差异有统计学意义(P < 0.01)。结论 ① 大连地区50例健康人nNO浓度值为(389.29±124.85)×10-9。②健康人可单侧鼻孔测量nNO代替双侧鼻孔。③安静状态下, 一定时间内延长鼻腔不通气时间, nNO浓度呈逐渐升高趋势, 继续延长鼻腔不通气时间, 有望为无创检测鼻窦内NO浓度及鼻窦口通畅性提供思路。Abstract: Objective To measure the normal value of nasal nitric oxide(nNO) concentration of 50 healthy adults in Dalian. By investigating the changing trend of nitric oxide accumulation concentration in the nasal cavity when changing the duration of nasal non-ventilation under quiet state, to provide ideas for the noninvasive detection of the concentration of nitric oxide in the sinus and the patency of the sinus ostium.Methods ① The left and right nNO concentrations(LnNO, RnNO) of 50 healthy subjects in Dalian were measured by Sunvou-CA2122(Wuxi), and the LnNO10, LnNO20, and LnNO30 were measured through the left nasal cavity when the the time of nasal non ventilation was set for 10 s, 20 s, 30 s respectively. Ten subjects with good cooperation extended the time of nasal non ventilation to 50 s, and the measured nNO value was denoted as LnNO50. ②Statistical analysis was performed by SPSS 19.0, and differences between two sides of nNO were analyzed by t-test, and the trend of changes in concentration of NO accumulation in the nasal cavity when changing the duration of nasal nonventilation was investigated by One-way repeated measures ANOVA.Results ① The mean LnNO concentration in 50 healthy subjects was (383.32±126.35) ×10-9, the mean RnNO concentration was (395.26±124.32) ×10-9, and there was no significant difference between the two side(P>0.05), and the mean nNO was (389.29±124.85) ×10-9. ②The mean nNO concentration measured at 10, 20, and 30 seconds nasal nonventilation were (984.54±477.69) ×10-9 for LnNO10, (1527.32±717.25) ×10-9 for LnNO20, (2183.26±946.21) ×10-9 for LnNO30, and(3083.00±1905.62) ×10-9 for LnNO50 measured at prolonging nasal nonventilation time to 50 seconds for ten of these subjects; The difference in the presence of increased nNO concentrations measured after prolonged nasal nonventilation was statistically significant(P < 0.01).Conclusion ① The values of nNO of 50 healthy individuals in Dalian were(389.29±124.85) ×10-9. ②In healthy individuals, nNO can be measured unilaterally instead of bilaterally. ③In quiet condition, the duration of nasal nonventilation was prolonged for a certain period of time, and the nNO concentration showed a gradually increasing trend. It is expected to provide ideas for noninvasive detection of NO concentration in nasal sinuses and patency of nasal sinuses.
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Key words:
- nasal cavity /
- nitric oxide /
- concentration
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表 1 大连地区50例健康成人nNO浓度统计
侧别 侧数 x±s 95%CI LnNO 50 383.32±126.35 347.41~419.23 RnNO 50 395.26±124.32 359.93~430.59 nNO 100 389.29±124.85 364.52~414.06 
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表 2 改变鼻腔不通气时间时NO在鼻腔的蓄积情况
nNO/
(×10-9)例数 鼻腔不通气
时间/sx±s LnNO 50 0 383.32±126.35 LnNO10 50 10 984.54±477.69 LnNO20 50 20 1527.32±717.25 LnNO30 50 30 2183.26±946.21
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表 3 改变鼻腔不通气时间时nNO的成对比较
nNO(I) nNO(J) 平均值差值(I-J) 标准差 P 95%CI LnNO LnNO10 -601.22 60.64 <0.01 -767.95~-434.49 LnNO10 LnNO20 -542.78 50.42 <0.01 -681.43~-404.13 LnNO20 LnNO30 -655.94 60.49 <0.01 -822.25~-404.13
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