18β-甘草次酸钠对变应性鼻炎大鼠鼻黏膜TNF-α的干预作用

折宁宁; 侯赟; 王有虎; 桂岩; 席克虎; 陈小婉; 陈康兵; 马春霞; 刘小涵; 张小兵

doi:10.13201/j.issn.1001-1781.2019.03.019

18β-甘草次酸钠对变应性鼻炎大鼠鼻黏膜TNF-α的干预作用

- 1. 兰州大学第一医院耳鼻咽喉头颈外科(兰州, 730000);
- 2. 兰州大学(兰州, 730000)
基金项目:
国家自然科学基金项目 (No:81160449)

详细信息

通讯作者: 张小兵,E-mail:790736924@qq.com

中图分类号: R765.21

收稿日期: 2018-10-10

Effects of 18β-sodium glycyrrhetinic acid on TNF-α expression in rats with allergic rhinitis

- 1. Department of Otolaryngology Head and Neck Surgery, the First Hospital of Lanzhou University, Lanzhou, 730000, China;
- 2. Lanzhou University

More Information

Corresponding author: ZHANG Xiaobing, E-mail: 790736924@qq.com

Received Date: 10 October 2018

摘要

摘要: 目的:观察18β-甘草次酸钠(18β-SGA)对变应性鼻炎(AR)大鼠鼻黏膜肿瘤坏死因子-α(TNF-α)表达的影响,探讨18β-SGA对AR的干预机制。方法:106只SPF级Wistar大鼠随机分为空白对照组、AR组、布地奈德组、18β-SGA低剂量组和18β-SGA高剂量组。经卵清蛋白构建AR大鼠模型后给予药物干预,各组大鼠分别在干预2周和4周后处死,取大鼠鼻黏膜行免疫组织化学染色、RT-PCR和免疫蛋白印迹定位、定量大鼠鼻黏膜中TNF-α表达。结果:经免疫组织化学、免疫印迹及RT-PCR发现,与空白对照组相比,AR组大鼠鼻黏膜中TNF-α高表达于鼻黏膜纤毛柱状上皮、血管内皮、腺体及部分炎症细胞胞质中(P<0.01)。经不同剂量的18β-SGA干预后,TNF-α表达量明显下降(P<0.01),尤以18β-SGA低剂量组干预4周后效果最明显(P<0.01)。结论:不同剂量的18β-SGA对AR有治疗作用,且其发挥作用的机制与抑制TNF-α的高表达有关。
- 18β-甘草次酸钠 /
- 鼻炎 /
- 变应性 /
- 肿瘤坏死因子-α
Abstract: Objective:To observe the effect of 18β-sodium glycyrrhetinic acid (18β-SGA) on the expression of TNF-αin nasal mucosa of rats with allergic rhinitis (AR), and explore the intervention mechanism of 18β-SGA on AR.Method:One hundred and six SPF-level Wistar rats were randomly divided into control group, AR group, budesonide group, 18β-SGA low dose group and high dose group.After the AR rat model was constructed by ovalbumin, the rats were given drug intervention and sacrificed after 2 and 4 weeks of intervention.The nasal mucosa of the rats was taken for immunohistochemical staining, RT-qPCR and Western-blotting to localize and quantify the expression of TNF-α.Result:By immunohistochemistry, Western-blotting and RT-PCR, TNF-αwas mainly found in the columnar epithelium, vascular endothelium, glandular and some inflammatory cytoplasm of nasal mucosa.And the expression of TNF-αin the nasal mucosa of AR rats was significantly increased than the normal group at the protein and mRNA levels (P<0.01).After intervention with different doses of 18β-SGA, the expression of TNF-αwas significantly decreased (P<0.01), especially after 4 weeks of 18β-SGA low dose group (P<0.01).Conclusion:Different doses of 18β-SGA have therapeutic effects on AR, and its mechanism of action may be related to the inhibition of TNF-αexpression.
- 18β-sodium glycyrrhetinic acid /
- rhinitis /
- allergic /
- tumor necrosisi factor-α

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