布地奈德对变应性鼻炎患者外周血树突状细胞免疫功能的影响

申震; 李白芽; 戴浩; 张少强; 白艳霞; 邵渊

doi:10.13201/j.issn.1001-1781.2018.18.007

布地奈德对变应性鼻炎患者外周血树突状细胞免疫功能的影响

- 西安交通大学第一附属医院耳鼻咽喉头颈外科(西安, 710061)

详细信息

通讯作者: 邵渊,E-mail:shaxiaoying1976@163.com

中图分类号: R765.21

收稿日期: 2018-05-02

Effects of budesonide aerosol inhalation on the immunological functions of peripheral dendritic cells in patients with allergic rhinitis

- Department of Otolaryngology Head and Neck Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, China

More Information

Corresponding author: SHAO Yuan, E-mail: shaxiaoying1976@163.com

Received Date: 02 May 2018

摘要

摘要: 目的：探讨布地奈德鼻喷雾剂治疗变应性鼻炎（AR）过程中患者外周血树突状细胞（DC）免疫功能的变化。方法：选取中重度持续性AR患者25例，给予布地奈德鼻喷雾剂治疗8周，评估患者治疗前、治疗4周和8周时患者AR相关症状和指标、外周血DC数量、比例以及活化程度的变化。结果：AR患者治疗4周和8周后症状评分、嗜酸粒细胞比例以及血清AR特异性抗体sIgE和T-IgE均下降，差异有统计学意义，治疗结束时总有效率达到88%。患者DC细胞数目和DC/PBMC较治疗前降低，差异有统计学意义（P<0.05）。与治疗前相比，AR患者治疗4周和8周后，活化DC细胞表面标志分子CD86和CD80均不同程度降低，而糖皮质激素诱导的亮氨酸拉链蛋白逐渐升高，均差异有统计学意义（P<0.05）。与治疗前相比，DC细胞分泌的IL-10的浓度随着治疗时间延长而降低，同时DC刺激T细胞分泌的IFN-γ水平在治疗后显著升高，均差异有统计学意义（P<0.05）。结论：布地奈德有助于抑制AR患者外周血DC的活化和成熟，这可能与其纠正DC参与的Th2反应从而减轻患者症状有关。
- 布地奈德气雾剂 /
- 鼻炎,变应性 /
- 树突状细胞 /
- 免疫功能
Abstract: Objective: To investigate the immunologic functional changes of peripheral dendritic cells (DC) in patients with allergic rhinitis (AR) during the treatment by budesonide aerosol inhalation.Method: 25 patients with moderate to severe persistent AR were concluded in current study, and they were given budesonide aerosol inhalation for 8 weeks. AR related nasal symptom scores and indices, peripheral DC counts and percentages as well as their activation before and 4 weeks, 8 weeks after treatment were determined.Result: There were significant decrease in nasal symptom scores, eosinophils, AR specific IgE antibodies after 4 and 8 weeks of treatment. The overall clinical efficiency reached 88% at the end of the treatment. Peripheral DC counts and DC/PBMC decreased in comparison with the levels of pre-treatment (P<0.05). Compared with the levels before treatment, DC activation markers CD86 and CD80 reduced to various extent, while glucocorticoid-induced leucine zipper (GILZ) positive cells gradually elevated (P<0.05). In the DC secreted IL-10 decreased against the treatment duration, while IFN-γ from DC conditioned T cells statistically increased after treatment (P<0.05). Conclusion: Budesonide suppressed DC activation and maturation, which might redirect DC-involved Th2 response, in turn alleviating the AR symptoms.
- budesonide aerosol /
- rhinitis,allergic /
- dendritic cells /
- immunologic functions

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