Nrf2和ERK通路在甲状腺乳头状癌中的表达及临床意义

陈亚敏; 魏仑; 林旭; 张文静; 吴靖芳; 薛刚

doi:10.13201/j.issn.1001-1781.2017.12.011

Nrf2和ERK通路在甲状腺乳头状癌中的表达及临床意义

- 1 河北北方学院基础医学院(张家口, 075000);
- 2 河北北方学院附属第一医院耳鼻咽喉头颈外科
基金项目:
河北省重大医学科研课题 (No:ZD2013052)

张家口市科技攻关计划 (No:1411043H, 1611055H)

详细信息

通讯作者: 吴靖芳,E-mail:wjfxg@163.com; 薛刚,E-mail:xgwjf@163.com

中图分类号: R736.1

收稿日期: 2017-02-11

Expression and clinical significance of Nrf2 and ERK pathway in papillary thyroid carcinoma

- 1 Basic Medical College, Zhangjiakou, 075000, China;
- 2 Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital, Hebei North University

More Information

Corresponding authors: WU Jingfang ; XUE Gang

Received Date: 11 February 2017

摘要

摘要: 目的:探讨核因子E2相关因子2(Nrf2)及ERK信号通路在甲状腺乳头状癌(PTC)中的表达及临床意义。方法:免疫组织化学法检测PTC组织芯片中Nrf2、ERK₂和p-ERK_1/2的表达;Western blot检测Nrf2、ERK₂、p-ERK_1/2蛋白在PTC、癌旁组织及正常甲状腺组织中的相对含量;实时定量PCR检测Nrf2 mRNA在PTC、癌旁组织及正常甲状腺组织中的转录水平。结果:Nrf2、ERK₂、p-ERK_1/2蛋白在PTC中高表达,在癌旁组织中弱阳性或阴性表达(P＜0.01)。Nrf2、ERK₂、p-ERK_1/2蛋白在淋巴结转移和临床Ⅲ~Ⅳ期组明显高于无淋巴结转移组和临床Ⅰ~Ⅱ期组(P＜0.05),且Nrf2蛋白与ERK₂和p-ERK_1/2蛋白呈正相关(r=0.723 2和r=0.668 6,P＜0.05)。Western blot结果显示PTC组织Nrf2、ERK₂、p-ERK_1/2含量高于癌旁和正常组织(P＜0.01)。PTC组Nrf2 mRNA转录水平明显高于癌旁及正常组织(P＜0.01)。结论:Nrf2与PTC的进展有关,推测Nrf2高表达与ERK₂通路有关。
- 核因子E2相关因子2 /
- 甲状腺乳头状癌 /
- ERK2 /
- p-ERK1/2
Abstract: Objective: To exlpore the expression and clinical significance of nuclear factor erythroid-2 related factor 2(Nrf2) and ERK sinal in papillary thyroid carcinoma(PTC).Method: PTC microassy were detected by immunohistochemical method(IHC) . The expression of Nrf2,ERK₂ and p-ERK_1/2 in PTC, para-carcimoma and normal thyroid tissue were detected by Western blot. The transcriptional level of Nrf2 mRNA in PTC, para-carcimoma and normal thyroid tissue were detected by real-time quantitative PCR(qPCR) method.Result: Nrf2,ERK₂,p-ERK_1/2 protein were high expression in group of lymph node metastasis PTC and negative or weak positve expression in para-carcinoma tissue(P＜0.01).And the proteins of clinical stage Ⅲ-Ⅳof PTC groups express higher than without lymph node metastasis group and clinical stage Ⅰ-Ⅱ of PTC group (P＜0.05). The expression of Nrf2 protein was positively correlated with ERK₂ and p-ERK_1/2 protein in PTC(r=0.723 2,r=0.668 6,P＜0.05). The Western blot results of Nrf2,ERK₂,p-ERK_1/2 had a higer level in PTC than those of paracarcinoma and normal thyroid tissue(P＜0.01).The level of Nrf2 mRNA were higher than those of paracarcinoma and normal thyroid tissue(P＜0.01).Conclusion: High expresion of Nrf2 may be associated with the progression of papillary thyroid carcinoma,and may relate to the high expression and activity of ERK pathway.
- nuclear factor erythroid-2 related factor 2 /
- papillary thyroid carcinoma /
- extracellular protein kinase 2 /
- p-ERK1/2

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