应用功能分类基因芯片检测变应性鼻炎差异性表达基因

彭杨; 李献清; 邱前辉

doi:10.13201/j.issn.1001-1781.2017.11.012

应用功能分类基因芯片检测变应性鼻炎差异性表达基因

- 1 南方医科大学研究生院(广州, 510515);
- 2 广东省人民医院耳鼻咽喉头颈外科
基金项目:
广东省自然科学基金项目 (No:S2013010016130)

广州市科技计划项目:产学研协同创新重大专项 (No:201604020187)

详细信息

通讯作者: 邱前辉,E-mail:qiuqianhui@hotmail.com

中图分类号: R765.21

收稿日期: 2017-01-18

Detection of differentially expressed geneof allergic rhinitis based on RT~2 profiler PCR array

- 1 Department of Graduate School, Southern Medical University, Guangzhou, 510515, China;
- 2 Department of Otolaryngology Head and Neck Surgery, Guangdong General Hospital and Guangdong Academy of Medical Sciences

More Information

Corresponding author: QIU Qianhui, E-mail: qiuqianhui@hotmail.com

Received Date: 18 January 2017

摘要

摘要: 目的: 应用功能分类基因芯片技术研究变应性鼻炎差异表达基因。方法: 分别提取、检测8例变应性鼻炎及8例正常对照的下鼻甲黏膜组织的总RNA,合成cDNA后进行实时荧光定量PCR芯片扫描,检测出两组间的差异表达基因。结果: 通过对84个与过敏及哮喘发病相关基因的分析,发现67个基因表达下调,17个基因表达上调,差异具有统计学意义的差异性表达基因为2个(STAT6、BCL6)。结论: 用功能分类基因芯片可检测变应性鼻炎中异常表达的基因。
- 鼻炎,变应性 /
- 功能分类基因芯片
Abstract: Objective: The aim of this study is to detect differentially expressed gene of allergic rhinitis(AR) based on RT² profiler PCR array.Method: The real-time PCR array containing 84 genes were used to compare inferior turbinate samples obtained from AR patients(n=8) and healthy individuals(n=8).Result: The results showed that 67 genes were down regulated and 17 genes were up regulated in AR inferior turbinate after analysis of 84 genes related to allergy and asthma.STAT6 and BCL6 were recognized as differentially expressed genes.Conclusion: The real-time PCR array can provide new insight into the pathophysiological processes involved in AR.
- rhinitis,allergic /
- real-time PCR array

HTML全文

参考文献(20)

[1]	SEIDMAN M D, GURGEL R K, LIN S Y, et al.Clinical practice guideline:Allergic rhinitis[J].Otolaryngol Head Neck Surg, 2015, 152 (1 Suppl):S1-43.
[2]	ZHANG Y, ZHANG L.Prevalence of allergic rhinitis in china[J].Allergy Asthma Immunol Res, 2014, 6:105-113.
[3]	WAGENER A H, ZWINDERMAN A H, LUITEN S, et al.The impact of allergic rhinitis and asthma on human nasal and bronchial epithelial gene expression[J].PLoS One, 2013, 8:e80257.
[4]	HANSEL N N, CHEADLE C, DIETTE G B, et al.Analysis of CD⁴⁺ T-cell gene expression in allergic subjects using two different microarray platforms[J].Allergy, 2008, 63:366-369.
[5]	中华耳鼻咽喉头颈外科杂志编辑委员会鼻科组, 中华医学会耳鼻咽喉头颈外科学分会鼻科学组.变应性鼻炎诊断和治疗指南 (2015年, 天津)[J].中华耳鼻咽喉头颈外科杂志, 2016, 51 (1):6-24.
[6]	中华医学会呼吸病学分会哮喘学组.支气管哮喘防治指南 (支气管哮喘的定义、诊断、治疗和管理方案)[J].中华哮喘杂志 (电子版), 2008, 2 (1):3-13.
[7]	章如新, 余少卿, 应康, 等.变应性鼻炎基因芯片检测及其基因表达谱的研究[J].中华耳鼻咽喉科杂志, 2002, 37 (3):165-168.
[8]	刘冰, 吴建, 赵舒薇, 等.人类变应性鼻炎基因表达谱的研究[J].中国耳鼻咽喉头颈外科, 2008, 15 (3):149-152.
[9]	WAGENER A H, ZWINDERMAN A H, LUITEN S, et al.The impact of allergic rhinitis and asthma on human nasal and bronchial epithelial gene expression[J].PLoS One, 2013, 8:e80257.
[10]	赵丹.STAT6基因2964G/A位点多态性与山东地区变应性鼻炎的关联性研究[D].山东大学, 2012.
[11]	VALE K.Targeting the JAK-STAT pathway in the treatment of'Th2-high'severe asthma[J].Future Med Chem, 2016, 8:405-419.
[12]	BONNELYKKE K, MATHESON M C, PERS T H, et al.Meta-analysis of genome-wide association studies identifies ten loci influencing allergic sensitization[J].Nat Genet, 2013, 45:902-906.
[13]	张慧, 耿曼英, 闫保星, 等.IL-4/STAT6在变应性鼻炎豚鼠鼻黏膜的表达及鼻用糖皮质激素对其表达的影响[J].临床耳鼻咽喉头颈外科杂志, 2012, 26 (8):364-367.
[14]	程超群, 刘涛.变应性鼻炎中调节性T细胞和Bcl-6的研究进展[J].中国医药指南, 2012, 10 (27):447-448.
[15]	HABERLE M T, KELSCH E, DORSCH K, et al.Knock-down of BCL6/STAT6 sensitizes primary B cell lymphoma cells for treatment with current therapeutic agents[J].Oncoscience, 2014, 1:283-286.
[16]	AUDZEVICH T, PEARCE G, BREUCHA M, et al.Control of the STAT6-BCL6 antagonism by SWAP-70determines IgE production[J].J Immunol, 2013, 190:4946-4955.
[17]	NURIEVA R I, CHUNG Y, MARTINEZ G J, et al.Bcl6 mediates the development of T follicular helper cells[J].Science, 2009, 325:1001-1005.
[18]	EIVAZI S, BAGHERI S, HASHEMZADEH M S, et al.Development of T follicular helper cells and their role in disease and immune system[J].Biomed Pharmacother, 2016, 84:1668-1678.
[19]	ZHANG Y N, SONG J, WANG H, et al.Nasal IL-4 (+) CXCR5 (+) CD4 (+) T follicular helper cell counts correlate with local IgE production in eosinophilic nasal polyps[J].J Allergy Clin Immunol, 2016, 137:462-473.
[20]	WANG X Q, KE X, SHEN Y, et al.Changes in circulating follicular helper T-cells in Chinese patients with allergic rhinitis[J].Acta Otolaryngol, 2016, 136:199-204.