组织工程技术复合修复兔口腔黏膜的实验研究

武欣; 祝威; 王苹; 傅仲鹰; 陈玮伦; 杨田田

doi:10.13201/j.issn.1001-1781.2016.22.009

组织工程技术复合修复兔口腔黏膜的实验研究

- 吉林大学第一医院耳鼻咽喉-头颈外科(长春, 130000)

详细信息

通讯作者: 祝威,E-mail:zhuwei30000@163.com

中图分类号: R392.2

收稿日期: 2016-06-01

Repairmen of the rabbit’s oral mucosa by tissue engineering technology

- Department of Otorhinolaryngology-Head and Neck Surgery, the First Hospital of Jilin University, Changchun, 130000, China

More Information

Corresponding author: ZHU Wei, E-mail: zhuwei30000@163.com

Received Date: 01 June 2016

摘要

摘要: 目的:探讨骨髓间充质干细胞(BMMSCs)与左旋聚乳酸/丝素蛋白(PLLA/SF)支架复合物修复兔口腔黏膜的可行性,同时评估PLLA与SF以何种比例混合构建组织工程口腔黏膜支架最理想。方法:分离、培养并鉴定BMMSCs,将其置于PLLA与SF的质量配比为70∶30(P70S30)PLLA/SF支架上复合培养1周后观察细胞表型有无改变;观察BMMSCs与P70S30的PLLA/SF支架复合物的口腔黏膜移植1周后的结构变化;比较细胞与支架复合物组、单纯支架组、创面自然修复组的创面愈合率。结果:①PLLA/SF支架对BMMSCs的细胞表型无明显影响;②BMMSCs与P70S30的PLLA/SF支架复合物口腔黏膜移植1周后与创面的融合较好;③以PLLA与SF的质量配比为70∶30的复合物组愈合率最高,平均愈合率为94.8%,与创面自然修复组及单纯支架组比较差异有统计学意义(P＜0.01)。结论:①BMMSCs与PLLA/SF支架复合物具有构建理想的组织工程口腔黏膜的潜力;②PLLA与SF的质量配比为70∶30时最利于口腔黏膜创面的愈合,构建理想的组织工程口腔黏膜的潜力最大。
- 口腔黏膜修复 /
- 骨髓间充质干细胞 /
- 左旋聚乳酸 /
- 丝素蛋白
Abstract: Objective: This experiment proposed to complicate BMMSCs and PLLA/SF scaffolds, to study its repairing ability for rabbit oral mucosa wound, and try to evaluate the most potential mixed proportion of PLLA and SF for tissue engineering.Method: Separating,cultivating and identifying BMMSCs. Observing cell phenotype of BMMSCs after cultivating BMMSCs in P70S30 PLLA/SF scaffold for one week.Observing the structure change of BMMSCs and P70S30PLLA/SF scaffold complexes after oral mucosa transplantation experiment for a week;Comparing the wound healing rate of composite of BMMSCs and PLLA/SF scafold,PLLA/SF scaffold and nature repair.Result: ①There wasn't a significant effect of PLLA/SF scaffold on cell phenotype of BMMSCs.②The wounds fused well with the oral mucosa transplanted composite of BMMSCs and P70S30 PLLA/SF scaffold after a week.③The healing rate of composite of BMMSCs and P70S30 scaffold was the highest.The average healing rate of composite of BMMSCs and P70S30 scaffold was 94.8%,whose difference was statistically significant compared with natural healing and pure scaffold(P＜0.01).Conclusion: ①The complex composed of BMMSCs and PLLA/SF scaffolds has the potential as an ideal scaffold for tissue engineering oral mucosa.②The complex composed of BMMSCs and P70S30 PLLA/SF scaffolds is the most beneficial to wound healing of oral mucosa,and has the greatest potential for constructing tissue engineering oral mucosa.
- oral mucosal repairment /
- bone marrow mesenchymal stem cell /
- poly-left-lactic acid /
- silk fibroin

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