Association of CTLA-4 gene polymorphism in allergic rhinitis with asthma or not in children
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摘要: 目的:探讨细胞毒T淋巴细胞相关抗原4(CTLA-4)基因多态性与儿童变应性鼻炎(AR)合并哮喘疾病易感性的关系。方法:单纯AR组302例,AR合并哮喘组288例,对照组327例,收集外周血标本提取DNA后进行病例对照研究。采用限制性片段长度多态性聚合酶链反应和直接测序法对CTLA-4基因的6个多态性位点(rs3087243,rs11571302,rs11571315,rs231725,rs35219727,rs4553808)进行基因分型。结果:各组在各个位点基因分型均符合哈迪温伯格平衡。位点rs3087243和rs231725AA基因型及A等位基因频率存在统计学差异。rs3087243AA基因型及A等位基因频率仅在AR合并哮喘组明显升高,rs231725AA基因型及A等位基因频率的改变在单纯AR组及AR合并哮喘组均有意义。结论:儿童AR合并哮喘的易感性与CTLA-4基因多态性有明显相关性,且rs3087243和rs231725位点单核苷酸多态性与儿童AR合并哮喘的易感性密切相关。Abstract: Objective:To evaluate the involvement of the cytotoxic T lymphocyte associated antigen (CTLA-4) gene polymorphisms in susceptibility of allergic rhinitis(AR) with/without asthma in children.Method:Three hundred and two AR children, 288 AR with asthma children, and 327 healthy Chinese Han children were enrolled in the study. The SNPs of CTLA-4(rs3087243, rs11571302, rs11571315, rs231725, rs35219727, and rs4553808) were sequenced using a PCR restriction fragment length polymorphism assay.Result:An increased frequency of the AA genotype and A allele in rs3087243 of CTLA-4 was found in AR with asthma group. For rs231725, AA genotype and A allele were obviously increased in AR group, while they were significantly decreased in AR with asthma group(P<0.05).Conclusion:We found a significant association between CTLA-4 gene polymorphism and AR with asthma in Chinese Han children, which might be an autoimmunity risk factor for AR with asthma.
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Key words:
- rhinitis /
- allergic /
- asthma /
- the cytotoxic T-lymphocyte-associated antigen 4 /
- child /
- polymorphism /
- single nucleotide
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