MiR-148a在鼻咽癌中的表达及其对肿瘤细胞生物学功能的影响

赵锦成; 石颖; 王宇; 张颖; 马新; 贾占红; 吴再军; 张京秋

doi:10.13201/j.issn.1001-1781.2016.15.010

MiR-148a在鼻咽癌中的表达及其对肿瘤细胞生物学功能的影响

- 陆军总医院263临床部耳鼻咽喉头颈外科(北京, 101149)

详细信息

通讯作者: 王宇,E-mail:wangyumike@126.com

中图分类号: R739.63

收稿日期: 2016-03-26

The expression of MiR-148a in nasopharyngeal carcinoma and its effect on tumor cell biology functions in nasopharyngeal carcinoma

- ENT Head and Neck Surgery 263 Clinical Department of General Army Hospital, Region, Beijing, 101149, China

More Information

Corresponding author: WANG Yu, E-mail: wangyumike@126.com

Received Date: 26 March 2016

摘要

摘要: 目的: 探讨MiR-148a在鼻咽癌细胞中的表达水平变化及其对鼻咽癌肿瘤细胞生物学功能的影响。方法: 采用Real-time PCR方法检测MiR-148a在鼻咽癌细胞系CNE2中的表达水平变化,并通过MTT实验、克隆形成实验和流式细胞术检测MiR-148a表达变化对鼻咽癌细胞的增殖和凋亡影响。通过生物信息学软件分析预测MiR-148a的下游靶向调控基因表皮生长因子受体(EGFR),Real-time PCR和Western blot检测EGFR表达水平变化。结果: 相对于正常鼻咽癌上皮组织细胞,MiR-148a在鼻咽癌细胞系CNE2中的表达水平显著降低。MTT实验、克隆形成实验和流式细胞术检测发现MiR-148a能够抑制细胞增殖,并促进细胞的凋亡。Real-time PCR和Western blot检测结果表明MiR-148a可降低EGFR的表达水平。结论: MiR-148a能够影响鼻咽癌细胞的增殖和凋亡,很有可能参与了鼻咽癌的发生发展过程。
- MiR-148a /
- 鼻咽肿瘤 /
- 生物学功能
Abstract: Objective: Nasopharyngeal carcinoma(NPC) is a common malignant tumor, in recent years, most of studies have found that micro RNA played an important role in the development of NPC. This study was to explore the expression level of MiR-148a and its effect on the biological functions of NPC cells.Method: The expression of MiR-148a in NPC cell line CNE2 was detected by Real-time PCR method. MTT, clone formation assay and flow cytometry were applied to detect cell proliferation and apoptosis. We predicted that EGFR was the downstream target genes of MiR-148a through the analysis of bioinformatics software. Then the expression change of EGFR was measured by Real-time PCR and Western blot.Result: Comparing with normal nasopharyngeal epithelial tissue cells, MiR-148a expression level was significantly reduced in NPC cell line CNE2. MTT, clone formation assay and flow cytometry test show that overexpression of MiR-148a can inhibit cell proliferation and promote cell apoptosis. Real-time PCR and Western blot test show that MiR-148a can reduce the expression of EGFR.Conclusion: MiR-148a can affect the proliferation and apoptosis of NPC cell, and it is likely to be involved in the development and progression of NPC.
- MiR-148a /
- nasopharyngeal neoplasms /
- biological functions

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