Abstract: Objective: To study the effect of low-dose gentamicin intratympanic therapy on intractable Meniere's disease on hearing.Method: One hundred and forty cases of patients with ENT intractable Meniere's disease were selected in this clinical trial study, all patients were enrolled in the drum line intraventricular injection of low-dose gentamicin treatment. After two weeks' treatment in hospital, the patients followed-up for 1 years, all patients did pure tone audiometry, speech audiometry and on hearing threshold changes before and after treatment were comparatively analyzed; continu followed-up for 1 year and the patients' Meniere's disease therapeutic effect were analyzed.Result: After treatment, the follow up within a year of 120 patients(140 cases, 20 cases were lost), 36 cases never attack of vertigo symptoms, the total effective rate was 91.7%; all patients before treatment speech frequency was(67.2±2.1) dB after treatment(65.5±2.0) dB, the difference was not statistically significant(P>0.05); In the group of gentamicin treatment, all patients 1-3 kHz compare hearing thresholds and changes of 8 kHz before and after injection, the difference was not statistically significant(P>0.05); tinnitus, ear fullness episodes of a significant reduction in the overall incidence rate dropped to 2% from 7% after treatment, the difference was statistically significant(P<0.05).Conclusion: For intractable Meniere's disease, a small dose of gentamicin intratympanic have a positive clinical effect, and no significant effect on hearing Thus, it is a worthy of promotion and application for low dose gentamincin intratympanic injection in intractable Meniere's disease.