DDR2和MMP-13在中耳胆脂瘤中的表达及意义

许连方; 孙文忠

doi:10.13201/j.issn.1001-1781.2016.12.004

DDR2和MMP-13在中耳胆脂瘤中的表达及意义

许连方¹,
孙文忠^2,3, ,

- 1 桂林医学院(广西桂林, 541000);
- 2 柳州市人民医院耳鼻咽喉头颈外科广西科技大学第二附属医院

详细信息

通讯作者: 孙文忠,E-mail:1278863616@qq.com

中图分类号: R739.61

收稿日期: 2016-02-15

The expression and significance of DDR2 and MMP-13 in human middle ear cholesteatoma

XU Lianfang¹,
SUN Wenzhong^2,3, ,

- 1 Guilin Medical University, Guilin, 541000, China;
- 2 Department of Otolaryngology Head and Neck Surgery, People's Hospital of Liuzhou City, the Second Affiliated Hospital of Guangxi University of Science and Technology

More Information

Corresponding author: SUN Wenzhong, E-mail: 1278863616@qq.com

Received Date: 15 February 2016

摘要

摘要: 目的: 研究在中耳胆脂瘤上皮组织中盘状结构域受体2(DDR2)、基质金属蛋白酶13(MMP-13)的表达水平,探讨两者在中耳胆脂瘤骨质破坏中的作用及相互关系。方法: 运用免疫组织化学SP法检测DDR2、MMP-13在30例中耳胆脂瘤上皮组织和10例外耳道正常上皮组织中的表达水平,并应用计算机图像分析系统对其表达进行定量分析,分析两者在中耳胆脂瘤上皮组织中的表达与骨质破坏程度之间的相互关系。结果: 在中耳胆脂瘤上皮组织中DDR2及MMP-13表达的平均光密度值显著高于外耳道正常上皮组织(P<0.05)。DDR2和MMP-13在中耳胆脂瘤上皮组织的表达呈正相关(r=0.738,P<0.01);且两者的表达与骨质破坏程度呈正相关,骨质破坏范围越广,二者的表达越高(P<0.05)。结论: DDR2和MMP-13在中耳胆脂瘤的骨质破坏中可能具有重要的作用。
- 盘状结构域受体2 /
- 基质金属蛋白酶-13 /
- 胆脂瘤 /
- 中耳
Abstract: Objective: To ascertain the expression level of discoidin domain receptors 2(DDR2) and matrix metalloproteinase-13(MMP-13) in human middle ear cholesteatoma tissues and further investigate their roles in bone destruction and correlation.Method: Using immunohistochemical S-P method to detect the expression level of DDR2,MMP-13 in 30 specimens with middle ear cholesteatoma epithelial tissue and 10 specimens with normal ear epithelial tissues.At the same time,the computer image analysis system was used to detect the expression of the two indexes by the quantitative analysis,analyzing their expression in middle ear cholesteatoma epithelial tissue and the correlation between the extent of bone destruction.Result: The expression of DDR2 and MMP-13 were confirmed in human middle ear cholesteatoma epithelial tissues and normal ear epithelial tissues. The mean optical density of DDR2 and MMP13 in human middle ear cholesteatoma epithelial tissues which were tested by the computer image quantitative analysis system were higher than those in normal ear epithelial tissues(P<0.05).The expression of DDR2 and MMP-13 in middle ear cholesteatoma epithelial tissues were positively correlated(r=0.738,P<0.01).In addition,the two indexes were associated and relative to the extent of bone destruction,the wider the extent of bone destruction was,the higher the expression of both indexes(P<0.05).Conclusion: DDR2 and MMP-13 may play important roles in bone destruction of human middle ear cholesteatoma.
- domain receptor 2 /
- matrix metalloproteinase-13 /
- cholesteatoma /
- middle ear

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