顺铂对豚鼠耳蜗及螺旋神经节细胞Bcl-2和Bax表达的影响

李鹏; 张威; 张奕; 符秋养; 孟庆翔; 谢景华; 梁勇

doi:10.13201/j.issn.1001-1781.2016.01.013

顺铂对豚鼠耳蜗及螺旋神经节细胞Bcl-2和Bax表达的影响

- 1. 南方医科大学附属南方医院耳鼻咽喉头颈外科(广州, 510515);
- 2. 广州市第一人民医院耳鼻咽喉科
基金项目:
广东省科技计划项目(No:2014A020212491)

详细信息

通讯作者: 梁勇,E-mail:yliang@fimmu.com

中图分类号: R764

收稿日期: 2015-10-06

Effectiveness of cisplatin on the expressions of Bcl-2 and Bax in cochlea and spiral ganglion cells of guinea pigs

- 1. Department of Otorhinolaryngology-Head and Neck Surgery, Nanfang Hospital of Southern Medical University, Guangzhou, 510515, China;
- 2. Department of Otorhinolaryngology, Guangzhou First People's Hospital

More Information

Corresponding author: LIANG Yong, E-mail: yliang@fimmu.com

Received Date: 06 October 2015

摘要

摘要: 目的:探讨顺铂对凋亡相关蛋白Bcl-2和Bax在豚鼠耳蜗及螺旋神经节细胞(SGC)中表达的影响。方法:选取健康豚鼠20只,随机分为顺铂组和对照组,每组各10只。顺铂组一次性腹腔注射顺铂16 mg/kg,对照组腹腔注射等量生理盐水。每组豚鼠给药前和给药后7 d均行畸变产物耳声发射(DPOAE)检测。Western Blot及免疫组织化学检测Bcl-2、Bax 在耳蜗及SGC中的表达。结果:对照组和顺铂组耳蜗Bcl-2蛋白表达水平分别为0.727 8±0.016 9和0.467 6±0.020 1,Bax蛋白表达水平分别为0.384 8±0.021 7和0.735 6±0.022 3,2组比较均差异有统计学意义(均P<0.01)。对照组和顺铂组SGC中Bcl-2的表达灰度值为99.00±2.42、149.80±2.37, Bax的表达灰度值为154.50±2.80、104.50±3.09,2组比较均差异有统计学意义(均P<0.05)。结论:与对照组比较,顺铂组Bcl-2表达下降而Bax表达增强,这可能是顺铂诱导豚鼠耳蜗及SGC凋亡的因素之一。
- Bcl-2 /
- Bax /
- 顺铂 /
- 螺旋神经节细胞
Abstract: Objective:To investigate the effectiveness of cisplatin on the expressions of Bcl-2 and Bax in cochlea and spiral ganglion cells (SGC) of guinea pigs.Method:Twenty guinea pigs were randomly divided into cisplatin (n=10) and control groups (n=10). Cisplatin group were administrated with a dose of intraperitoneal injection of 16 mg/kg, while the control group were received intraperitoneal injection of normal saline as placebo. Before and 7 days following injections, the ototoxic effect was measured with distortion product otoacoustic emission (DPOAE).Bcl-2, Bax in cochlea were detected by Western Blot. Immunohistochemical staining was used to detect the protein levels of Bcl-2 and Bax in spiral ganglion cells.Result:In control and cisplatin group, Bcl-2 protein levels were 0.727 8±0.016 9 and 0.467 6±0.020 1, Bax protein levels were 0.384 8±0.021 7 and 0.735 6±0.022 3 in cochlea respectively,both P<0.01. In Control and cisplatin group, the grey values of Bcl-2 in SGC were 99.00±2.42 and 149.80±2.37 respectively, the grey values of Bax were 154.50±2.80 and 104.50±3.09 respectively, both P<0.05.Conclusion:Decreased expression of Bcl-2 and increased expression of Bax may be involved in cisplatin-induced apoptosis in cochlea and SGC of guinea pigs.
- Bcl-2 /
- Bax /
- cisplatin /
- spiral ganglion cells

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