MMP-2在喉鳞状细胞癌组织中的表达及其与预后的关系

周彬; 侯学东; 时胜武

doi:10.13201/j.issn.1001-1781.2015.23.014

MMP-2在喉鳞状细胞癌组织中的表达及其与预后的关系

- 沈阳医学院附属中心医院耳鼻咽喉科
基金项目:
沈阳医学院科学研究基金(No:20111020)

详细信息

通讯作者: 周彬,E-mail:Zhoub1965@sina.com

中图分类号: R739.65

收稿日期: 2015-09-16

Relationship between expression of MMP-2 and prognosis in human laryngeal squamous cell carcinoma

- Department of Otorhinolaryngology, Affiliated Central Hospital of Shenyang Medical College, Shenyang, 110024, China

More Information

Corresponding author: ZHOU Bin, E-mail: Zhoub1965@sina.com

Received Date: 16 September 2015

摘要

摘要: 目的: 探讨基质金属蛋白酶2(MMP-2)作为潜在的对人喉鳞状细胞癌的预后因子的作用。方法: 回顾性分析73例进行手术切除的患者肿瘤组织MMP-2的表达。免疫组织化学染色用链霉素抗生物素蛋白过氧化物酶复合法进行。术后随访观察MMP-2表达及临床病理特征,包括年龄、性别、临床分型、病理类型、淋巴结转移和预后之间的关系,均采用SPSS 19.0统计学软件分析。结果: 73例患者MMP-2的阳性表达率为57.53%(42/73)。Kaplan-Meier分析显示MMP-2表达阴性组和表达阳性组间的5年总生存率分别为76.0%和57.5%,差异有统计学意义(P<0.05)。原发灶部位、临床分期、淋巴结转移、T分级与喉癌预后相关(P分别为0.002、0.009、0.034、0.001);病理分化、年龄和性别等与喉癌预后没有统计学意义上的相关性。结论: MMP-2表达与较差的生存相关,可以考虑作为一个潜在的预后不良的标志物。
- 喉肿瘤 /
- 基质金属蛋白酶2 /
- 预后
Abstract: Objective: In order to evaluate the potential of matrix metalloproteinase 2(MMP-2) as a prognostic factor for human laryngeal squamous cell carcinoma (HLSCC).Method: Seventy-three surgical specimens from patients with HLSCC were reviewed retrospectively regarding MMP-2 expression via immunohistochemistry. Immunostaining was performed using a streptavidin-biotin peroxidase complex technique. The patients were followed-up till June 2014 and the relationship between MMP-2 and clinical data including age, gender, metastasis, clinical type, pathological type, lymph node metastasis and prognosis were analyzed using SPSS 19.0.Result: The positive expression rate of MMP-2 in 73 patients was 57.53%(42/73). Kaplan-Meier analysis demonstrated statistically significant difference for 5-year overall survival rate between the group with positive and negative MMP-2 expression,the 5-year overall survival rate were 76.0% and 57.5% respectively in the group with negative and positive MMP-2 expression.The site of the primary tumor, clinical stage, lymph node metastasis and T grade were related to the prognosis of HLSCC (P were 0.002, 0.009, 0.034 and 0.001 respectively), and there was no significant correlation between age, sex, pathological differentiation and prognosis of HLSCC.Conclusion: MMP-2 was related with worse overall disease survival and could be considered as a potential marker of poor prognosis.
- laryngeal neoplasms /
- MMP-2 /
- prognosis

HTML全文

参考文献(16)

[1]	RIGGINS K S, MERNAUGH G, SU Y, et al. MT1-MMP-mediated basement membrane remodeling modulates renal development[J]. Exp Cell Res,2010,316:2993-3005.
[2]	OTA I, LI X Y, HU Y, et al. Induction of a MT1-MMP and MT2-MMP-dependent basement membrane transmigration program in cancer cells by Snail1[J]. Proc Natl Acad Sci U S A, 2009,106:20318-20323.
[3]	段炳烽,冯耀光.基质金属蛋白酶与恶性肿瘤侵袭转移关系的研究进展[J].现代医药卫生, 2012,28(12):1853-1855.
[4]	TURPEENNIEMI-HUJANEN T. Gelatinases (MMP-2 and 9) and their natural inhibitors as prognostic indicators in solid cancers[J]. Biochimie, 2005, 87:287-297.
[5]	CHRISTOPOULOS T A, PAPAGEORGAKOPOULOU N, RAVAZOULA P, et al. Expression of metalloproteinases and their tissue inhibitors in squamous cell laryngeal carcinoma[J]. Oncol Rep, 2007,18:855-860.
[6]	SAUSSEZ S, CLUDTS S, CAPOUILLEZ A, et al. Identification of matrix metalloproteinase-9 as an independent prognostic marker in laryngeal and hypopharyngeal cancer with opposite correlations to adhesion/growth-regulatory galectins-1 and-7[J]. Int J Oncol,2009, 34:433-439.
[7]	DAVIS S J, DIVI V, OWEN J H, et al. Metastatic potential of cancer stem cells in head and neck squamous cell carcinoma[J]. Arch Otolaryngol Head Neck Surg,2010, 136:1260-1266.
[8]	张玉玲,谷京城.喉癌术后生存率相关因素的研究进展[J].临床耳鼻咽喉头颈外科杂志,2010,24(4):189-192.
[9]	WERNER J A, RATHCKE I O, MANDIC R, et al. The role of matrix metalloproteinases in squamous cell carcinomas of the head and neck[J]. Clin Exp Metastasis, 2002,19:275-282.
[10]	许熠铭,谢民强,刘涛,等.基质金属蛋白酶MMP-1和MMP-2与喉癌侵袭和淋巴结转移的关系[J].中国现代医学杂志,2010,20(3):1930-1934.
[11]	汤夏冰,沈晓辉,钱晓云,等.高迁移率族蛋白B1和基质金属蛋白酶-2、9在喉癌组织中的表达及其与预后的关系[J].临床耳鼻咽喉头颈外科杂志,2013,27(4):181-187.
[12]	MALLIS A, TEYMOORTASH A, MASTRONIKOLIS N S, et al. MMP-2 expression in 102 patients with glottic laryngeal cancer[J]. Eur Arch Otorhinolaryngol, 2012,269:639-642.
[13]	LIU W W, ZENG Z Y, WU Q L, et al. Overexpression of MMP-2 in laryngeal squamous cell carcinoma:a potential indicator for poor prognosis[J]. Otolaryngol Head Neck Surg,2005,132:395-400.
[14]	GÖRÖGH T, BEIER U H, BÄUMKEN, et al. Metalloproteinases and their inhibitors:influence on tumor invasiveness and metastasis formation in head and neck squamous cell carcinomas[J]. Head Neck,2006,28:31-39.
[15]	KRECICKI T, ZALESSKA-KRECICKA M, JELEN M, et al. Expression of type IV collagen and matrix metalloproteinase-2(type IV collagenase) in relation to nodal status in laryngeal cancer[J]. Clin Otolaryngol Allied Sci, 2001, 26:469-472.
[16]	DANILEWICZ M, SIKORSKA B, WAGROWSKA-DANILEWICZ M. Prognostic significance of the immunoexpression of matrix metalloproteinase MMP2 and its inhibitor TIMP2 in laryngeal cancer[J]. Med Sci Monit, 2003, 9:MT42-MT47.