骨桥蛋白与喉鳞状细胞癌侵袭转移的相关研究

赵洪春; 王延飞; 郑体花; 车娟; 曹瑞娟; 王霞

doi:10.13201/j.issn.1001-1781.2015.17.010

骨桥蛋白与喉鳞状细胞癌侵袭转移的相关研究

- 1 滨州医学院附属医院耳鼻咽喉头颈外科(山东滨州, 256603);
- 2 滨州医学院耳鼻咽喉神经研究所;
- 3 滨州医学院附属医院病理科
基金项目:
山东省滨州市科技发展计划(No:2011ZC0916)

详细信息

通讯作者: 王延飞,E-mail:gogozhc@163.com

中图分类号: R739.6

收稿日期: 2015-05-18

Correlation of osteopontin expression and laryngeal squamous cell carcinoma infiltration and metastasis

- 1 Department of Otorhinolaryngology-Head and Neck Surgery, the Affiliated Hospital of Binzhou Medical College, Binzhou, 256603, China;
- 2 Ent Neurological Institute of Binzhou Medical College;
- 3 Department of Pathology, the Affiliated Hospital of Binzhou Medical College

More Information

Corresponding author: WANG Yanfei, E-mail: gogozhc@163.com

Received Date: 18 May 2015

摘要

摘要: 目的:探讨骨桥蛋白(OPN)在喉鳞状细胞癌患者血浆及组织中的表达水平及其与喉癌侵袭、转移的相关性,评价OPN对喉鳞状细胞癌的临床意义。方法:采用酶联免疫吸附法(ELISA)和免疫组织化学法分别检测OPN在60例喉鳞状细胞癌患者的血浆及组织中的表达,并用20例健康体检者的血浆及20例癌旁正常喉组织做对照。结果:血浆OPN水平喉鳞状细胞癌患者组为(38.089±9.225)ng/ml,健康对照组为(18.563±9.308)ng/ml,组间比较差异有统计学意义(P<0.05);血浆OPN水平与喉鳞状细胞癌的颈部淋巴结转移、临床分期相关(P<0.05),而与肿瘤部位、病理分级及性别、年龄无明显相关性(P>0.05)。60例喉鳞状细胞癌组织OPN的阳性表达率为56.67%,高于喉癌旁正常组织(15.00%),两者比较差异有统计学意义(P<0.05);喉癌组织中OPN蛋白的表达与喉鳞状细胞癌的临床分期(P<0.05)、病理分级(P<0.05)、颈部淋巴结转移(P<0.05)有关。喉鳞状细胞癌患者血浆OPN的水平与组织中OPN的表达呈正相关(r=0.871,P<0.05)。结论:OPN的高表达在喉鳞状细胞癌的侵袭、转移及病情进展中起重要作用,因此联合检测血浆和组织中OPN的表达可作为判断喉鳞状细泡癌侵袭、转移的指标。
- 骨桥蛋白 /
- 免疫组织化学 /
- 喉鳞状细胞癌
Abstract: Objective: To investigate osteopontin (OPN) expression in plasma and tissue of patients with laryngeal squamous cell carcinoma and analyze its role in invasion, metastasis, and clinical significance in laryngeal squamous cell carcinoma.Method: Enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry were used to detect expression of OPN in plasma and tissue of 60 cases of laryngeal squamous cell carcinoma, 20 cases of adjacent normal laryngeal tissue and 20 cases of plasma from healthy subjects.Result: The expression of plasma OPN was closely correlated with clinical stage and cervical lymphatic metastasis in laryngeal squamous cell carcinoma (P<0.05), but no significant correlation with the tumor location, pathological grade, gender and age (P>0.05). The expression of OPN increased in plasma during cancer development: laryngeal squamous cell carcinoma (38.089±9.225)ng/ml, healthy subjects (18.563±9.308)ng/ml. There was a significant difference between the groups (P<0.05). The expression of OPN in tissue was closely correlated with clinical stage (P<0.05), pathological grade (P<0.05) and cervical lymphatic metastasis (P<0.05) in laryngeal squamous cell carcinoma adjacent atypical hyperplastic epithelium and carcinoma. The expression of OPN increased in tissue during cancer development: laryngeal squamous cell carcinoma (56.67%), adjacent normal laryngeal tissue (15.00%). There was a significant difference between the groups (P<0.05). Elevated expression of plasma OPN is positively correlated with the expression of OPN in tissue in laryngeal squamous cell carcinoma patients (r=0.871,P<0.05).Conclusion: OPN plays an important role in the infiltration, metastasis and carcinogenesis in laryngeal squamous cell carcinoma. Combination of serum OPN, tissue OPN detection can be used as diagnostic and surveillance indicators for laryngeal squamous cell carcinoma infiltration and metastasis.
- osteopontin /
- immunohistochemistry /
- laryngeal squamous cell carcinoma

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