Tiam 1基因对头颈部鳞状细胞癌细胞增殖及迁移的影响

肖平; 符秋养; 李泽泳; 张学辉; 傅向军; 万仁强

doi:10.13201/j.issn.1001-1781.2015.07.015

Tiam 1基因对头颈部鳞状细胞癌细胞增殖及迁移的影响

- 1. 广东省第二人民医院耳鼻咽喉-头颈外科(广州, 510317);
- 2. 广东省第二人民医院生物实验中心
基金项目:
广东省医学科研基金资助项目(No:A2014151)

详细信息

通讯作者: 肖平,E-mail:34212763@qq.com

中图分类号: R739.91

收稿日期: 2014-12-11

Effect of Tiam 1 on cell proliferation and migration in head and neck squamous cell carcinoma cells

- 1. Department of Otorhinolaryngology-Head and Neck Surgery, Second People's Hospital of Guangdong, Guangzhou, 510317, China;
- 2. Department of Biology Experiment Center, Second People's Hospital of Guangdong

More Information

Corresponding author: XIAO Ping, E-mail: 34212763@qq.com

Received Date: 11 December 2014

摘要

摘要: 目的:探讨Tiam 1基因过表达对头颈部鳞状细胞癌(HNSCC)细胞增殖及迁移能力的影响。方法:采用荧光定量RT-PCR方法检测Tiam 1基因在HNSCC细胞株中的表达;利用慢病毒介导Tiam 1基因在人HNSCC细胞株UM-SCC-47中过表达,G418筛选抗性克隆,荧光定量RT-PCR和Western blot法鉴定转导效果。利用细胞计数和四甲基偶氮唑盐法检测Tiam 1对细胞增殖的影响,划痕实验检测Tiam 1对HNSCC细胞侵袭转移的影响。结果:Tiam 1基因在高转移性HNSCC细胞株M2中呈高表达,在低转移性细胞株UM-SCC-47中呈低表达。细胞计数和MTT结果显示,过表达Tiam 1后能显著促进细胞增殖(P<0.05),划痕实验证明Tiam 1可显著促进HNSCC细胞迁移(P<0.05)。结论:Tiam 1基因具有促进HNSCC细胞增殖与迁移的能力,Tiam 1基因表达可作为HNSCC侵袭转移过程中一个有价值的指标。
- T淋巴瘤侵袭转移诱导因子1 /
- 细胞增殖 /
- 细胞迁移 /
- 癌 /
- 鳞状细胞 /
- 头颈部肿瘤
Abstract: Objective:To investigate the effect of T lymphoma invasion and metastasis 1 (Tiam 1) overexpression in head and neck squamous cell carcinoma (HNSCC) cells.Method:Endogenous expression of Tiam l in 8 head and neck squamous cell carcinoma cell(HNSCC) lines was investigated by real-time RT-PCR. A lentivirus vector containing Tiam1 was transfected into UM-SCC-47 cells, a head and neck squamous cell carcinoma cell line with little endogenous Tiaml expression. Stable clone, obtained by G418 screening, were assayed by RT-PCR and Western blot to validate the gene expression efficiency. The biological behaviors of the transduced cells were determined by cell counting, MTT and in-vitro migration assay.Result:Tiam l gene was highly expressed in M2 cell line and it's low level expression was found in UM-SCC-47. Cell counting and MTT assay showed that over-expression of Tiam1 significantly promoted cell proliferation (P<0.05). The cell monolayers overexpressed Tiam1 that resulted in a significant increasment of cell migration in infected head and neck squamous cell carcinoma cell lines (P<0.05).Conclusion:Tiam l gene plays an important role in the growth and migration in head and neck squamous cell carcinoma cell lines. It may be a useful marker for metastasis of head and neck squamous cell carcinoma.
- Tiam 1 /
- cell proliferation /
- cell migration /
- carcinoma /
- squanous cell /
- head and neck neoplasms

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