成人嗅黏膜嗅鞘细胞的体外培养及迁移特性

朱秋蓓; 刘环海; 郎军添; 彭浒; 赵舒薇

doi:10.13201/j.issn.1001-1781.2015.05.008

成人嗅黏膜嗅鞘细胞的体外培养及迁移特性

- 第二军医大学附属长征医院耳鼻咽喉科(上海, 200003)
基金项目:
国家自然科学基金青年项目(No:81300837)

详细信息

通讯作者: 赵舒薇,E-mail:zhaoshw1@yahoo.com.cn

中图分类号: R765.6

收稿日期: 2014-10-17

The cultivation and migration in vitro of olfactory ensheathing cells from human olfactory mucosa

- Department of Otolaryngology, Changzheng Hospital, the Second Military Medical University, Shanghai, 200003, China

More Information

Corresponding author: ZHAO Shuwei, E-mail: zhaoshw1@yahoo.com.cn

Received Date: 17 October 2014

摘要

摘要: 目的: 探讨成人嗅黏膜嗅鞘细胞的分离、培养及体外迁移特性。方法: 分离并培养成人及大鼠鼻腔嗅黏膜来源的嗅鞘细胞,分别观察2组嗅鞘细胞的生长、分型以及体外迁移能力。结果: 成功分离培养成人和大鼠嗅黏膜嗅鞘细胞,成人嗅黏膜嗅鞘细胞的生长、分型、体外迁移能力及迁移特点与大鼠黏膜嗅鞘细胞相比无明显差异。结论: 成人嗅黏膜嗅鞘细胞与大鼠嗅黏膜嗅鞘细胞相比,具有类似的细胞特性,可以作为临床细胞移植修复神经损伤的可靠的细胞来源。
- 嗅鞘细胞 /
- 嗅黏膜 /
- 体外培养 /
- 迁移
Abstract: Objective: To investigate the bionomics of the olfactory ensheathing cells (OECs) of human olfactory mucosa.Method: To separate and cultivate the OECs of human and rat olfactory mucosa. To observe the cell growth, cell grouping and cell migration in vitro of the two types of OECs.Result: Successfully separated and cultivated the OECs of human and rat olfactory mucosa. OECs of the human and rat olfactory mucosa had the similar cell growth, cell grouping and cell migration ability in vitro.Conclusion: OECs of the human and rat olfactory mucosa have the similar bionomics in vitro, as a result, OECs of the human olfactory mucosa could be a reliable source of cell transplant for nerve injury.
- olfactory ensheathing cell /
- olfactory mucosa /
- cultivation in vitro /
- migration

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参考文献(20)

[1]	RAMON-CUETO A,AVILA J.Olfactory ensheathing glia:properties and function[J].Brain Res Bull,1998,46:175-187.
[2]	FARBMAN A I.Olfactory neurogenesis:genetic or environmental controls[J].Trends Neurosci,1990,13:362-365.
[3]	DOUCETTE R.Glia influences on axonal growth in the primary olfactory system[J].Glia,1990,33:433-449.
[4]	RAISMAN G,LI Y.Repair of neural pathways by olfactory ensheathing cells[J].Nat Rev Neurosci,2007,8:312-319.
[5]	LIPSON A C,WIDENFALK J,LINDQVIST E,et al.Neurotrophic properties of olfactory ensheathing glia[J].Exp Neurol,2003,180:167-171.
[6]	LU J.Transplantation of nasal olfactory tissue promotes partial recovery in paraplegic adult rats[J].Brain Res,2001,889:344-357.
[7]	GUNTINAS-LICHIU S O,WEWETZER K,TOMA L.Transplantation of olfactory mucosa minimizes axonal branching and promotes the recovery of vibrissae motor performance after facial nerve repair in rats[J].J Neuroscience,2002,22:7121-7131.
[8]	HARSHA R J,GEOFFREY R.Ensheathing cell cultures from the olfactory bulb and mucosa[J].Glia,2004,47:130-137.
[9]	FERON F,PERRY C,MCGRATH J J,et al.New techniques for biopsy and culture of human olfactory epithelial neurons[J].Arch Otolaryngol Head Neck Surg,1998,124:861-866.
[10]	ROHAN K,SHAISTA H,PAUL F,et al.Functional differences and interactions between phenotypic subpopulations of olfactory ensheathing cells in promoting cns axonal regeneration[J].Glia,2005,48:12-20.
[11]	ZHIDA S,CHENG H.Olfactory ensheathing cells:Biology in neural development and regeneration[J].Pro Neurobiol,2010,92:517-532.
[12]	YOU H,WEI L,LIU Y,et al.Olfactory ensheathing cells enhance Schwann cell-mediated anatomical and functional repair after sciatic nerve injury in adult rats[J].Exp Neurol,2011,229:158-167.
[13]	GUÉROUT N,DUCLOS C,DROUOT L,et al.Transplantation of olfactory ensheathing cells promotes axonal regeneration and functional recovery of peripheral nerve lesion in rats[J].Muscle Nerve,2011,43:543-551.
[14]	KUBASAK M D,JINDRICH D L,ZHONG H,et al.OEG implantation and step training enhance hindlimb-stepping ability in adult spinal transected rats[J].Brain,2008,131:264-276.
[15]	RADTKE C,WEWETZER K,REIMERS K,et al.Transplantation of olfactory ensheathing cells as adjunct cell therapy for peripheral nerve injury[J].Cell Transplant,2011,20:145-152.
[16]	CHOI D,LI D Q,LAW S,et al.A Prospective observational study of the yield of olfactory ensheathing cells cultured from biopsies of septal nasal mucosa[J].Neurosurgery,2008,62:1140-1145.
[17]	MACKAY-SIM A,JAMES A,JOHN S.Olfactory ensheathing cells from the nose:Clinical application in human spinal cord injuries[J].Exp Neurol,2011,229:174-180.
[18]	BIANCO J I,PERRY C,HARKIN D G,et al.Neuro-trophin 3promotes purlfication and proliferation of olfactory ensheathing cells from human nose[J].Glia,2004,45:111-123.
[19]	RICHTER M W,FLETCHER P A,LIU J,et al.Lamina Propria and olfactory bulb ensheathing cells exhibit differential integration and migration and promote differential axon sprouting in the lesioned spinal cord[J].J Neurosci,2005,25:10700-10711.
[20]	CAO L,SU Z D,ZHOU Q,et al.Glial cell line-derived neurotrophic factor promotes olfactory ensheathing cells migration[J].Glia,2006,54:536-544.