Dicer1低表达与鼻咽癌患者预后的关联性研究

许珊; 姜学钧

doi:10.13201/j.issn.1001-1781.2015.02.009

Dicer1低表达与鼻咽癌患者预后的关联性研究

许珊,
姜学钧^,

- 中国医科大学附属第一医院耳鼻咽喉科(沈阳, 110001)

详细信息

通讯作者: 姜学钧,E-mail:jiangxj_ln@163.com

中图分类号: R739.63

收稿日期: 2014-05-22

Reduced expression of Dicer1 is associated with poor prognosis in patients with nasopharyngeal carcinoma

XU Shan,
JIANG Xuejun^,

- Department of Otolaryngology, the First Affiliated Hospital of China Medical University, Shenyang, 110001, China

More Information

Corresponding author: JIANG Xuejun, E-mail: jiangxj_ln@163.com

Received Date: 22 May 2014

摘要

摘要: 目的:Dicer1在RNA干扰的起始阶段起着重要作用,本研究旨在评估Dicer1在鼻咽癌组织中的表达及预后价值。方法:采用免疫组织化学染色法检测276例鼻咽癌活检标本的Dicer1蛋白表达,利用定量RT-PCR法分析56例鼻咽癌活检标本和11例鼻咽炎组织中Dicer1 mRNA水平,采用Cox回归分析确定独立预后因素,建立预后评分模型进行生存预测。结果:Dicer1的mRNA和蛋白在鼻咽癌组织中表达水平均明显偏低,Dicer1的mRNA表达水平与蛋白表达水平呈正相关。Dicer1的低表达与鼻咽癌患者远处转移的发生(P<0.01)以及患者死亡率(P<0.05)显著相关。Dicer1低表达与鼻咽癌患者的总体生存期(HR=2.32,95% CI:1.30~4.14,P<0.01)和无远处转移生存率(HR=2.56,95% CI:1.39~4.74,P<0.01)亦显著相关。此外,多变量分析显示Dicer1低表达和TNM分期是鼻咽癌患者总体生存期和无远处转移生存率的独立预后因素。Dicer1表达和TNM分期相结合的预后评分模型比Dicer1表达单独模型或TNM分期单独模型拥有更好的预后价值(P<0.05)。结论:Dicer1 mRNA和蛋白表达水平在鼻咽癌组织中均明显偏低,Dicer1的低表达可能是鼻咽癌患者新的预后标志物。
- Dicer1 /
- 低表达 /
- 鼻咽癌 /
- 预后 /
- 生物标志物
Abstract: Objective: Dicer1 plays an important role in generation of microRNA, the purpose of this study was to evaluate Dicer11 expression and its prognostic value in nasopharyngeal carcinoma (NPC). Method: The protein expression of Dicer1 was examined by immunohistochemistry in 276 NPC specimens, and the mRNA levels of Dicer1 were analyzed by qRT-PCR in 56 NPC and 11 nasopharyngitis tissues. Cox regression analysis was used to identify independent prognostic factors, and a prognostic score model was constructed for survival prediction. Result: Expression of Dicer1 was downregulated in NPC tissues at both the mRNA and the protein levels, and there was a notable positive correlation between the expression levels of Dicer1 mRNA and protein. Low Dicer1 expression was positively correlated with distant metastasis (P<0.01) and death (P<0.05). In addition, low expression of Dicer1 was significantly associated with poorer overall survival (HR=2.32, 95%CI: 1.30~4.14, P<0.01) and poorer distant metastasis-free survival (HR=2.56, 95%CI: 1.39~4.74, P<0.01). Furthermore, multivariate analysis showed that low expression of Dicer1 and tumor-node-metastasis (TNM) stage were independent prognostic indicators for NPC patients. A prognostic score model combining the Dicer1 expression and TNM stage had a better prognostic value than the TNM stage alone model or Dicer1 expression alone model (P<0.05). Conclusion: Dicer1 was downregulated in NPC tissues at both the mRNA and the protein levels, and low expression of Dicer1 could be served as novel prognostic biomarker for NPC patients.
- dicer1 /
- reduced expression /
- nasopharyngeal carcinoma /
- prognosis /
- biomarker

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