LMP2A、E-Cadherin和fibronectin在鼻咽癌中的表达及其临床意义

赵利容; 饶洁; 罗泊涛; 陈小毅

doi:10.13201/j.issn.1001-1781.2015.02.010

LMP2A、E-Cadherin和fibronectin在鼻咽癌中的表达及其临床意义

- 1 广东医学院病理学教研室(广东湛江, 524023);
- 2 宜宾卫生学校

详细信息

通讯作者: 罗泊涛; 陈小毅,E-mail:18924333954@189.cn

中图分类号: R739.63

收稿日期: 2014-04-08

Expression of LMP2A,E-Cadherin and fibronectin in nasopharyngeal carcinoma and its clinical significance

- 1 Department of Pathology, Guangdong Medical College, Zhanjiang, 524023, China;
- 2 Yibin Health School

More Information

Corresponding authors: LUO Botao ; CHEN Xiaoyi

Received Date: 08 April 2014

摘要

摘要: 目的:探讨EBV编码的潜伏膜蛋白2A(LMP2A)与上皮-间质转化(EMT)标志物E-钙粘素(E-Cadherin)和纤维连接蛋白在鼻咽癌(NPC)中的表达及与临床病理学参数的关系。方法:采用免疫组织化学SP法检测LMP2A、E-Cadherin和fibronectin蛋白在32例鼻咽黏膜慢性炎、56例NPC和18例NPC淋巴结转移灶中的表达。结果:①LMP2A在NPC及其淋巴结转移灶中的阳性表达率分别为89.3%(50/56)和77.8%(14/18),均明显高于鼻咽黏膜慢性炎(37.5%,12/32)(均P<0.01);E-Cadherin在NPC及其淋巴结转移灶中的正常表达率分别为33.9%(19/56)和5.6%(1/18),均明显低于鼻咽黏膜慢性炎(90.6%,29/32)(均P<0.01);fibronectin在NPC及其淋巴结转移灶中的阳性表达率分别为83.9%(47/56)和72.2%(13/18),均明显高于鼻咽黏膜慢性炎(28.1%,9/32)(均P<0.01)。②NPC中LMP2A与E-Cadherin正常表达呈负相关(r=-0.387,P<0.01)、与fibronectin表达呈正相关(r=0.421,P<0.01)。③LMP2A、E-Cadherin和fibronectin表达与NPC患者N分期和临床分期密切相关(均P<0.05),与M分期无相关性(P>0.05);LMP2A、E-Cadherin表达与T分期也密切相关(均P<0.01)。结论:LMP2A、fibronectin在NPC中表达升高,而E-Cadherin正常表达则降低;LMP2A可能通过下调E-Cadherin和上调fibronectin表达来介导EMT促进NPC淋巴结转移和恶性进展。
- 鼻咽肿瘤 /
- 潜伏膜蛋白2A /
- 上皮-间质转化
Abstract: Objective: To investigate the expression of EBV-encoded latent membrane protein 2A (LMP2A) and epithelial-mesenchymal transformation(EMT) associated markers (E-cadherin and fibronectin) in nasopharyngeal carcinoma (NPC) and its clinical significance.Method: The expression of LMP2A, E-cad-herin and fibronectin proteins in 32 cases of chronic nasopharyngeal inflammat-ion, 56 cases of NPC and 18 cases of NPC lymph node metastasis were examined byimmunohistochemical SP method.Result: ①The positive rates of LMP2A in NPC and its lymph node metastasis were significantly higher than those of chronic nasopharyngeal inflammation (89.3%vs 37.5% and 77.8% vs 37.5%) respectively (both P<0.01); The normal expression rates of E-cadherin in NPC and its lymph node metastasis were significantly lower than those of chronic nasopharyngeal inflammation (33.9% vs 90.6% and 5.6% vs 90.6%) respectively (both P<0.01); The positive rates of fibronectin in NPC and its lymph node metastasis were significantly higher than those of chronic nasopharyngeal inflammation(83.9% vs 28.1% and 72.2% vs 28.1%) respectively (both P<0.01).②LMP2A expression were negatively correlated with normal expression of E-cadherin (r=-0.387,P<0.01), and were positively correlated with fibronectin (r=0.421,P<0.01).③LMP2A, E-cadherin and fibronectin expression were significantly correlated with N stage and clinical stage (both P<0.05), but the three proteins were not significantly correlated with M stage (both P>0.05).In addition, LMP2A and E-cadherin expression were significantly correlated with T stage (both P<0.01).Conclusion: LMP2A and fibronectin expressions were increased in NPC, but normal expression of E-cadherin were decreased. LMP2A may promote lymph node metastasis and malignant progression of NPC by induce EMT through downregulation of E-cadherin and upregulation of fibronectin.
- nasopharyngeal neoplasms /
- EBV-encoded latent membrane protein 2A /
- epithelial-mesenchymal transformation

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