微生态环境对小鼠变应性鼻炎发病率的影响

王敏; 宋勇莉; 王继猛; 闫辉; 米文娟; 邱建华; 乔莉

doi:10.13201/j.issn.1001-1781.2014.23.019

微生态环境对小鼠变应性鼻炎发病率的影响

- 1 第四军医大学西京医院耳鼻咽喉头颈外科(西安, 710032);
- 2 第四军医大学西京医院骨科
基金项目:
国家自然科学基金资助项目(No:81470031)

详细信息

通讯作者: 乔莉,E-mail:qiaoli@fmmu.edu.cn

中图分类号: R765.21

收稿日期: 2014-04-08

Effect of micro-ecological environment on incidence of allergic rhinitis on mice

- 1 Department of Otolaryngology Head and Neck Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China;
- 2 Department of Orthopaedics, Xijing Hospital, Fourth Military Medical University

More Information

Corresponding author: QIAO Li, E-mail: qiaoli@fmmu.edu.cn

Received Date: 08 April 2014

摘要

摘要: 目的:探讨微生态环境的改变对小鼠变应性鼻炎(AR)发病率的影响。方法:将60只新生的BALB/c小鼠随机分为2组,一组置于超净无菌环境(GF环境组),另一组置于无特定病原体环境(SPF环境组)中饲养,每组30只。2种环境中的小鼠再各分为模型组(20只)和对照组(10只)。2组环境组中的模型组各在6周龄时用卵清蛋白致敏并激发,观察其症状和体征并评分;于第9周末次激发实验后取材,用苏木精-伊红染色法行鼻腔组织切片染色;应用ELISA法检测外周血血清中IgE、IFN-γ和IL-4浓度。结果:各组小鼠症状体征评分显示,GF环境模型组中AR反应阳性体征的小鼠数量显著高于SPF环境模型组;苏木精-伊红染色显示AR反应阳性小鼠鼻黏膜高度充血水肿,有大量炎性细胞浸润,各模型组中AR反应阴性的小鼠和对照组的小鼠鼻黏膜无异常形态;嗜酸粒细胞计数显示AR反应阳性小鼠鼻黏膜内嗜酸粒细胞明显增多;ELISA结果显示AR反应阳性小鼠血清中IgE、IL-4浓度显著升高(P<0.05),IFN-γ浓度显著降低(P<0.05)。结论:GF环境中小鼠AR的发病率高于SPF环境。
- 鼻炎 /
- 变应性 /
- 微生态环境 /
- IgE /
- 干扰素γ /
- 白细胞介素4
Abstract: Objective:This study was designed to find out the impact of micro-ecological environment on the incidence of allergic rhinitis after developing a model of allergic rhinitis on mice.Method:Sixty mice were randomly divided into GF group (n=30) and SPF group (n=30). Mice of GF group were fed in the germ-free environment and mice of SPF group were fed in the specific pathogen-free environment. Then each group were randomly divided into model group (20 mice) and control group (10 mice).Establish allergic rhinitis model in the mice of model group using ovalbumin (OVA) at the age of 6 weeks, observe and score the corresponding symptoms and signs that could been induced. Stain with hematoxylin eosin (HE) staining method for nasal mucosa to observe the morphological changes. Using enzyme linked immunosorbent assay to detect the concentration of IgE, IFN-γ and IL-4 in the peripheral blood serum.Result:The chi square test showed that the incidence of allergic rhinithis in the mice of GF group was significantly higher than that in the SPF group (P<0.05).HE staining showed that the nasal mucosas of allergic rhinitis positive reaction mice were highly congestive and edematous and had a large number of inflammatory cell infiltration, while there was no abnormal morphology of nasal mucosas in mice with no allergic rhinitis reaction. EOS counting displayed that the number of eosinophilic cells in nasal mucosa of positive allergic rhinitis reaction mice was increased significantly. The concentration of IgE and IL-4 in the serum of positive allergic rhinitis reaction mice was highly increased (P<0.05), and IFN-γ was significantly decreased (P<0.05).Conclusion:The difference of micro-ecological environment may play a key role in the occurrence of allergic rhinitis in mice.
- rhinitis /
- allergic /
- micro-ecological environment /
- IgE /
- IFN-γ /
- IL-4

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