高渗性海水和等渗性海水对变应性鼻炎小鼠鼻黏膜的影响

邓智锋; 许昱; 欧劲; 向荣; 陶泽璋

doi:10.13201/j.issn.1001-1781.2014.23.018

高渗性海水和等渗性海水对变应性鼻炎小鼠鼻黏膜的影响

- 武汉大学人民医院耳鼻咽喉头颈外科(武汉, 430060)
基金项目:
国家自然科学基金资助项目(No:81371070)

湖北省自然基金项目(No:2012FFB04434)

详细信息

通讯作者: 许昱,E-mail:xy37138@163.com

中图分类号: R765.21

收稿日期: 2014-07-20

The effect of hypertonic seawater and isotonic seawater for nasal mucosa of allergic rhinitis mice model

- Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China

More Information

Corresponding author: XU Yu, E-mail: xy37138@163.com

Received Date: 20 July 2014

摘要

摘要: 目的:研究高渗性海水(HS)和等渗性海水(IS)对变应性鼻炎(AR)小鼠鼻黏膜的影响,并初步探讨海水鼻腔盥洗治疗AR的机制。方法:将Der p1构建的BALB/c小鼠AR动物模型随机分为3组:HS组、IS组和空白对照组(BC组)。HS组和IS组造模完成后第1~14天分别给予希诺宁(NaCl 2.3%)和必通(NaCl 0.9%)滴鼻,BC组造模完成后不予任何处理。阴性对照组不予任何处理,4组均在第14天末次给药后30 min内计数小鼠喷嚏及挠鼻次数;并在末次给药24 h后取各组小鼠鼻部组织固定,苏木精-伊红染色,通过透射电镜观察鼻黏膜微观结构。结果:AR小鼠经HS和IS滴鼻后,挠鼻及喷嚏次数较阴性对照组明显减少(P<0.05);HS组、IS组小鼠鼻黏膜组织中嗜酸粒细胞与BC组比较差异无统计学意义(P>0.05);HS组、IS组小鼠鼻黏膜纤毛柱状上皮细胞排列整齐,明显好于BC组,并且HS组小鼠鼻黏膜形态和微观结构较IS组更接近阴性对照组。结论:HS和IS鼻腔盥洗均可以促进损伤的鼻黏膜纤毛上皮修复,而且HS鼻腔盥洗促进损伤的鼻黏膜纤毛上皮修复作用好于IS。海水鼻腔盥洗治疗AR的作用机制可能是通过促进损伤的鼻黏膜纤毛上皮修复,从而减轻鼻部症状。
- 鼻炎 /
- 变应性 /
- 高渗性海水 /
- 等渗性海水 /
- 鼻黏膜
Abstract: Objective:To study the effect of hypertonic seawater and isotonic seawater for nasal mucosa of allergic rhinitis mice model, and explore the possible mechanism of nasal irrigation with seawater in treatment of allergic rhinitis.Method:We used Der p1 to make allergic rhinitis model of BALB/c mice, and divided them into three groups randomly.Nasal irrigation with hypertonic seawater (HS) or isotonic seawater (IS) in the treatment group 1-14 days after modeling, and black control (BC) group was given no treatment after modeling.Normal control (NC) group was given no treatment, the number of rubs and sneezings in each group were counted in 30 min after the last nasal irrigation. Mice were then killed 24 h after the last therapy. The noses of mice from each group were removed and fixed, then the slices were stained with hematoxylin and eosin, the others were observed by transmission electron microscope.Result:Mice with hypertonic seawater and isotonic seawater were significantly improved in rubs and sneezings compared to the black control group (P<0.05);The number of eosinophiles in mucosal tissues of HS group and IS group had no significant difference with that of the black control group (P>0.05);Ciliated columnar epithelium cells in mucosal tissues of HS group and IS group were arranged trimly, better than that in the black control group. Morphology and microstructure in nasal mucosal of HS group was closer to the normal group than in IS group.Conclusion:The injury of nasal mucosa ciliated epithelium was significantly improved by nasal irrigation with hypertonic seawater and isotonic seawater, and the former is better than the latter, the mechanism of nasal irrigation with seawater in treatment of allergic rhinitis may rely on repairing the injured nasal mucosa ciliated epithelium,thereby the symptoms of nasal was reduced.
- rhinitis allergic /
- hypertonic seawater /
- isotonic seawater /
- nasal mucosa /

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