鼻敏感颗粒对变应性鼻炎小鼠树突状细胞免疫调节机制的研究

吴继勇; 严道南; 马华安; 朱芸; 张世中; 胡瑶

doi:10.13201/j.issn.1001-1781.2014.13.018

鼻敏感颗粒对变应性鼻炎小鼠树突状细胞免疫调节机制的研究

- 1. 江苏省中医院耳鼻咽喉科(南京, 210029);
- 2. 南京中医药大学第一临床医学院
基金项目:
国家"十一五"科技支撑计划项目"变应性鼻炎中医治疗方案研究"（No：2007BAI20B037）；江苏省中医院科技基金项目（No：Y11001）

详细信息

通讯作者: 严道南,E-mail:yandaon2006@163.com

中图分类号: R765.4

收稿日期: 2013-07-26

Research of bimingan granule on dendritic cell in allergic rhinitis mice

- 1. Department of Otolaryngology, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, 210029, China;
- 2. The First Medical College, Nanjing University of Chinese Medicine

More Information

Corresponding author: YAN Daonan, E-mail: yandaon2006@163.com

Received Date: 26 July 2013

摘要

摘要: 目的：研究鼻敏感颗粒对变应性鼻炎小鼠树突状细胞（DC）发挥免疫调节作用的具体机制。方法：分离出小鼠骨髓细胞，使用rGM-CSF和rIL-4共培养骨髓细胞，刺激骨髓细胞向DC转化。然后加入脂多糖和不同浓度的鼻敏感颗粒共刺激未成熟DC，12 h后检测DC中MHCⅡ、CD80、CD86表面分子以及细胞上清中TNF-α、IL-1β、IL-6和IL-10的表达。结果：鼻敏感颗粒能够显著抑制成熟DC标志性细胞因子IL-6、TNF-α、IL-10、IL-1β的生成和成熟DC标志物MHCⅡ、CD80和CD86的表达。结论：鼻敏感颗粒能够显著抑制未成熟DC向成熟DC的分化。
- 鼻炎,变应性 /
- 树突状细胞
Abstract: Objective: To investigate the mechanism.Method: We isolate the mouse bone marrow cells and cultured with rGM-CSF and rIL-4 to stimulate bone marrow cells to transfer to immature dendritic cells. And then the immature dendritic cells were costimulated with LPS and different concentrations of Bimingan Granule.Result: MHCⅡ, CD80, CD86 were detected by flow cytometer and TNF-α, IL-1β, IL-6 and IL-10 were quantified by ELISA.Conclusion: Our results showed that Bimingan Granule may significantly inhibit the differentiation of immature dendritic cells to mature dendritic cells.
- rhinitis,allergic /
- dendritic cells

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参考文献(9)

[1]	吴继勇, 马华安, 朱珠, 等.益气温阳方对变应性鼻炎患者外周血IL-4、IFN-γ等细胞因子的影响[J].中国中西医结合耳鼻咽喉科杂志, 2011, 21(5):311-313.
[2]	JØRGENSEN T N, HAASE C, MICHELSEN B K. Treatment of an immortalized APC cell line with both cytokines and LPS ensures effective T-cell activation in vitro[J]. Scand J Immunol, 2002, 56:492-503.
[3]	JIN Y, FULLER L, CIANCIO G. Antigen presentation and immune regulatory capacity of immature and mature-enriched antigen presenting (dendritic) cells derived from human bone marrow[J]. Hum Immunol, 2004, 65:93-103.
[4]	KONDO Y, TACHIKAWA E, OHTAKE S. Inflammatory cytokines decrease the expression of nicotinic acetylcholine receptor during the cell maturation[J]. Mol Cell Biochem, 2010, 333:57-64.
[5]	李云英, 刘森平.耳鼻咽喉科专病中医临床诊治[M].2版.北京:人民卫生出版社, 2005:141-141.
[6]	严道南, 马华安, 郭小红, 等.益气温阳方对变应性鼻炎肺脾虚寒证的临床疗效[J]. 南京中医药大学学报, 2011, 27(1):19-21.
[7]	严道南, 吴继勇, 马华安, 等.益气温阳方治疗变应性鼻炎肺脾虚寒证的临床研究[J].南京中医药大学学报, 2012, 28(6):509-512.
[8]	王宇, 严道南.脾气虚与变应性鼻炎模型大鼠相关机理的研究[J].南京中医药大学学报, 2011, 27(2):140-143.
[9]	OKANO M, AZUMA M, YOSHINO T, et al. Differential role of CD80 and CD86 molecules in the induction and the effector phase of allergic rhintis in mice[J]. Am J Respir Care Med, 2001, 164:1501-1507.