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摘要: 目的：筛选及验证鼻咽癌中microRNAs（miRs）的差异表达谱。方法：通过芯片高通量表达谱分析及大规模微阵列技术，筛选鼻咽癌与炎症组织中差异表达的miRs，并应用RT-QPCR方法验证筛选结果的可信性。结果：鼻咽癌组织与对照组织的miRs存在差异表达，其中差异倍数大于2倍的人属miRs共有144种。RT-QPCR发现与炎症组织相比，癌组织中miRs-34b、miRs-449b、miRs-7-1表达显著下调，而miRs-125b、miRs-184、miRs-196b、miRs-205及miRs-24-1表达上调，结果与芯片分析结果相一致。结论：差异表达的miRs可能与鼻咽癌的发生、发展密切相关，对鼻咽癌miRs表达谱的研究可能为鼻咽癌的早期诊断及治疗提供有力的靶向依据。Abstract: Objective: To filtrate and prove the different microRNAs(miRs) profiles in nasopharyngeal carcinoma. Method: Screening the different expressions of miRs between nasopharyngeal carcinoma and the inflammatory tissues by the application of expression profiling of chip high-throughput and large-scale microarray analysis. Then we used RT-QPCR technology to prove the accuracy of screening results. Result: There were significant expression differences of miRs between nasopharyngeal carcinoma and the control tissues, 144 human miRs had 2 or more fold the difference ratio. Compared with the inflammatory tissues, we have found that miRs-34b, miRs-449b and miRs-7-1significantly low expressed in nasopharyngeal carcinoma, yet miRs-125b, miRs-184, miRs-196b, miRs-205 and miRs-24-1 expressed high. The results were consistent with the microarray analysis. Conclusion: The difference expressed miRs might be closely related to the process of nasopharyngeal carcinoma, and the research on miRs profiles maybe provide a powerful target basis for early diagnosis and therapy of nasopharyngeal carcinoma.
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Key words:
- nasopharyngeal carcinoma /
- microRNAs /
- profiles
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