白细胞介素-24对喉癌细胞的增殖抑制效应及机制

王睿; 王郡甫; 刘娣; 苏庆红; 周鹏; 陈雪梅; 许晓群

doi:10.13201/j.issn.1001-1781.2014.12.019

白细胞介素-24对喉癌细胞的增殖抑制效应及机制

- 1. 菏泽市立医院神经外科(山东菏泽, 274000);
- 2. 山东省医学科学院基础医学研究所;
- 3. 滕州市中心人民医院检验科;
- 4. 山东大学第二医院耳鼻咽喉科
基金项目:
山东省优秀中青年科学家科研奖励基金（No：BS2009SW007）；山东省自然科学基金（No：ZR2010CM067，ZR2009CM036，ZR2012HL23）；山东省大型科学仪器升级改造技术研究专项（No：2013SJGZ09）

详细信息

通讯作者: 陈雪梅,E-mail:chxmsd@163.com; 许晓群,E-mail:xuxiaoqunsd@163.com

中图分类号: R739.65

收稿日期: 2013-12-02

The inhibitory effect of interleukin-24 on the proliferation of laryngeal cancer cells and its mechanism

- 1. Department of Neurosurgery, Heze Municipal Hospital, Heze, 274000, China;
- 2. Institute of Basic Medicine, Shandong Academy of Medical Sciences;
- 3. Laboratory Medicine, Tengzhou Central People's Hospital;
- 4. Department of Otolaryngology, the Second Hospital of Shandong University

More Information

Corresponding authors: CHEN Xuemei ; XU Xiaoqun

Received Date: 02 December 2013

摘要

摘要: 目的：研究腺病毒介导的人白细胞介素-24（Ad-mda-7/IL-24）基因对喉癌细胞Hep-2增殖抑制效应及机制。方法：将携带有hIL-24基因的重组复制缺陷型腺病毒（Ad-hIL-24）感染喉癌细胞Hep-2，并以正常人脐静脉内皮细胞HUVEC为对照，采用RT-PCR方法检测Hep-2、HUVEC细胞中外源性hIL-24表达，以及Bcl-2和Bax的变化；采用四甲基偶氮唑蓝（MTT）比色法检测细胞的生长和凋亡情况。结果：腺病毒介导的hIL-24 mRNA能在Hep-2细胞和HUVEC细胞中表达；在Hep-2细胞中，抗凋亡分子Bcl-2表达降低，而促凋亡分子Bax表达增强；在HUVEC细胞中Bcl-2表达没有变化，Bax表达有所增强。MTT法显示Ad-hIL-24能明显抑制喉癌细胞Hep-2的生长。在Hep-2细胞中，显微镜下可见明显的细胞凋亡。结论：Ad-hIL-24能抑制喉癌细胞Hep-2生长和诱导Hep-2细胞凋亡，但对正常细胞无毒性作用。
- 白细胞介素-24 /
- 腺病毒 /
- 喉癌细胞 /
- 凋亡
Abstract: Objective: To study the inhibitory effect of adenovirus mediated interleukin-24 gene on the proliferation of laryngeal cancer Hep-2 cells and its mechanism.Method: The human IL-24 gene was transfected into human laryngeal cancer cell line Hep-2 and human umbilical vein endothelial cell (HUVEC) with a replication-incompetent adenovirus vector. The hIL-24 gene expression was confirmed by RT-PCR in both Hep-2 cells and HUVEC cells. The expression of Bcl-2 and Bax were detected by RT-PCR and Western blot. The cell proliferation and the apoptotic effect were tested by MTT assay and fluorescence microscopy. Result: The expression of hIL-24 were confirmed in Hep-2 cells and HUVEC cells by RT-PCR. RT-PCR and Western blot showed the expression of Bcl-2 significantly decreased in Hep-2 cells transfected with Ad-hIL-24 but not in transfected HUVEC cells, while the expression of Bax was increased in both transfected cells. MTT assay indicated that Ad-hIL-24 could induce proliferation inhibition in Hep-2 cells but not in HUVEC cells. Obvious apoptosis wan observed in Hep-2 cells under the microscope.Conclusion: Ad-hIL-24 can selectively inhibit proliferation and induces apoptosis of Hep-2 cells. However, it has no toxic effects on normal cells.
- adenovirus /
- interleukin-24 /
- laryngocarcinoma cell /
- apoptosis

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