苦参碱对人鼻咽癌CNE2细胞的生长抑制作用及凋亡相关基因Caspase表达的研究

谢貌; 何光耀; 石书婧; 刘奕康; 陈俊; 唐安洲

doi:10.13201/j.issn.1001-1781.2014.09.002

苦参碱对人鼻咽癌CNE2细胞的生长抑制作用及凋亡相关基因Caspase表达的研究

- 1 广西医科大学第一附属医院耳鼻咽喉头颈外科(南宁, 530021);
- 2 广西中医药大学药学院
基金项目:
国家自然科学基金项目 (No:81241113)

教育部高等学校博士学科点专项科研基金资助项目 (No:20094503110003)

广西教育厅立项项目 (No:桂教科研201106LX088)

详细信息

通讯作者: 唐安洲, E-mail:anzhoutang@126.com

中图分类号: R739.63

收稿日期: 2013-10-31

The inhibited effect of matrine on human nasopharyngeal carcinoma CNE2 cells and the expression of apoptosis related gene Caspase in vitro

- 1 Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China;
- 2 Pharmaceutical College, Guangxi University of Chinese Medicine

More Information

Corresponding author: TANG Anzhou, E-mail: anzhoutang@126.com

Received Date: 31 October 2013

摘要

摘要: 目的:观察苦参碱对人鼻咽癌CNE2细胞的生长抑制及对凋亡相关基因Caspase-3 mRNA及Caspase-3、Caspase-8和Caspase-9蛋白表达的影响,探讨苦参碱抑制人鼻咽癌细胞增殖和诱导其凋亡的机制。方法:采用MTT法检测不同浓度(0、0.25、0.50、0.75、1.00、1.50、2.00 g/L)苦参碱对CNE2细胞增殖的影响;采用荧光PCR法检测不同浓度(0、0.75、1.00、1.50 g/L)苦参碱处理48 h后Caspase-3 mRNA的变化;Western Blot检测Caspase-3、Caspase-8和Caspase-9蛋白的变化情况。结果:苦参碱对CNE2细胞具有明显的抑制作用,呈浓度依赖性;能上调CNE2细胞Caspase-3 mRNA和Caspase-3、Caspase-8、Caspase-9蛋白的表达,且呈浓度依赖性。结论:苦参碱能抑制CNE2细胞的生长,呈浓度依赖性。其作用与上调CNE2细胞Caspase-3 mRNA和Caspase-3、Caspase-8、Caspase-9蛋白的表达,启动Caspase级联反应密切相关。
- 苦参碱 /
- 鼻咽癌 /
- 凋亡 /
- Caspase
Abstract: Objective:To evaluate the inhibited effect of matrine on human nasopharyngeal carcinoma CNE2 cells and the expression of apoptosis-related gene Caspase-3 mRNA,Caspase-3,Caspase-8,Caspase-9 protein. And to explore the inhibiting effect of matrine on the apoptosis of human nasopharyngeal carcinoma CNE2 cells.Method:In vitro experiments,MTT assay was used to detect the effect of matrine on CNE2 cell proliferation with different concentration. The expression levels of Caspase-3 were detected by real-time PCR. Western Blot was used to gauge the levels of Caspase-3,Caspase-8 and Caspase-9 protein.Result:MTT results showed significant inhibitory action of matrine on CNE2 cells proliferation in does-dependent manner, which could up-regulate the expression of Caspase-3 mRNA and Caspase-3,Caspase-8,Caspase-9 protein in a dose-dependent manner.Conclusion:Matrine could inhibit CNE2 cells proliferation in a does-dependent,that was closely related to the up-regulation of Caspase-3 mRNA and Caspase-3, Caspase-8 and Caspase-9 protein expression, and to the cascade reaction of Caspase.
- matrine /
- nasopharyngeal carcinoma /
- apoptosis /
- Caspase

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