Cyclin D1在EGCG抗鼻咽癌中表达的变化及意义

栗子芳; 王文华; 何晓松; 宣广旭; 易世江; 张伟; 黄岚珍

doi:10.13201/j.issn.1001-1781.2014.09.001

Cyclin D1在EGCG抗鼻咽癌中表达的变化及意义

- 1 桂林医学院附属医院耳鼻咽喉头颈外科桂林医学院耳鼻咽喉头颈外科研究所(广西桂林, 541001);
- 2 桂林医学院科学实验中心
基金项目:
广西壮族自治区桂林市科学研究与技术开发项目 (No:20120121111)

详细信息

通讯作者: 何晓松, E-mail:hexiaosonggx@sina.com

中图分类号: R739.63

收稿日期: 2014-01-07

Signifinace of Cyclin D1 expression in CNE2 cells processed by EGCG

- 1 Department of Otorhinolaryngology-Head and Neck Surgery, the Affiliated Hospital of Guilin Medical College, the Institute of Otolaryngology of Guilin Medical College, Guilin, 541001, China;
- 2 The Science Experiment Center of Guilin Medical College

More Information

Corresponding author: HE Xiaosong, E-mail: hexiaosonggx@sina.com

Received Date: 07 January 2014

摘要

摘要: 目的:探讨Cyclin D1基因在表没食子儿茶素没食子酸酯(EGCG)抗鼻咽癌中表达的变化及意义,揭示EGCG的抗鼻咽癌作用机制。方法:体外培养低分化鼻咽癌细胞株CNE-2并以不同浓度EGCG处理,倒置显微镜下观察不同浓度EGCG作用48 h后CNE-2细胞的形态变化,采用MTT比色法检测细胞增殖抑制率,流式细胞仪检测细胞周期,半定量逆转录PCR检测细胞中Cyclin D1 mRNA的表达变化。结果:EGCG处理后,CNE-2细胞的数量及密度逐渐降低,分裂象减少,贴壁差,部分细胞变圆且体积变小,漂浮及凋亡细胞不断增多;细胞增殖明显受到抑制,CNE-2细胞被阻滞在G₀/G₁期,呈时间和剂量依赖性(P<0.05);Cyclin D1 mRNA表达明显下调,且EGCG作用浓度与时间依赖性(P<0.05)。结论:EGCG抑制CNE-2细胞的增殖,可能与其呈浓度与时间依赖性下调Cyclin D1 mRNA的表达相关。
- 表没食子儿茶素没食子酸酯 /
- CyclinD1 /
- 鼻咽癌 /
- 增殖
Abstract: Objective:To study the expression of Cyclin D1 in nasopharyngeal carcinoma cells processed by epigallocatechin gallate(EGCG) and it's significance, and revealed the anti-tumor mechanism of EGCG against nasopharyngeal carcinoma. Method:CNE-2 cells were treated by EGCG at different concentrations, the morphological changes of CNE-2 cells were observed by inverted microscope; the inhibition ratio of cell proliferation was detected by MTT colorimetric method, flow cytometry was used to analyze the changes of cell cycle. The expression of Cyclin D1 mRNA was detected by RT-PCR. Result:After treated by EGCG, the CNE2 cells decreased in amount and density,some of which became roll and small; Floating and dead cells can be seen in the inverted microscopy; cell proliferation was significantly inhibited in a time and dose dependent (P<0.05). CNE-2 cells were arrested at G₁/G₀ phase. The expression of Cyclin D1 mRNA was down-regulated by EGCG with concentration and action time dependent (P<0.05). Conclusion:EGCG resisted nasopharyngeal carcinoma by inhibiting the cell proliferation, The down regulation of Cyclin D1 mRNA expression in a time and dose dependent may be the possible mechanisms.
- epigallocatechin gallate /
- Cyclin D1 /
- nasopharyngeal carcinoma /
- proliferation

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