鼻咽癌组织中6-磷酸果糖激酶-1基因的表达及临床意义

李烁; 杜政德; 高进良; 高春生; 刘飞; 杨琼; 刘邦华

doi:10.13201/j.issn.1001-1781.2014.06.010

鼻咽癌组织中6-磷酸果糖激酶-1基因的表达及临床意义

- 1 华中科技大学同济医学院附属协和医院耳鼻咽喉科(武汉, 430022);
- 2 广东医学院附属深圳南山医院耳鼻咽喉科
基金项目:
深圳市南山区科技局基金资助 (No:2012031)

详细信息

通讯作者: 刘邦华, E-mail:drliubh@gmail.com

中图分类号: R739.63

收稿日期: 2013-06-03

Expression and clinical significance of 6-phosphofructo-1-kinase in nasopharyngeal carcinoma

- 1 Department of Otolaryngology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China;
- 2 Department of Otolaryngology, Nanshan Affiliated Hospital of Guangdong Medical College

More Information

Corresponding author: LIU Banghua, E-mail: drliubh@gmail.com

Received Date: 03 June 2013

摘要

摘要: 目的:探讨糖酵解限速酶6-磷酸果糖激酶-1(PFK1)基因在鼻咽癌活检标本中的表达及意义。方法:采取病例-对照设计,鼻咽癌活检标本取自41例鼻咽癌患者,对照组取自20例鼻咽部慢性炎性患者鼻咽部活检组织,采取实时定量PCR(RT-PCR)检测PFK1mRNA 的表达。结果:鼻咽部活检组织中,鼻咽癌组织中PFK1mRNA 表达水平明显高于鼻咽部炎性组织;PFK1mRNA在伴有淋巴结转移的鼻咽癌活检组织中表达明显高于无淋巴结转移的鼻咽癌活检组织。结论:PFK1可能是鼻咽癌发生和转移的分子标志之一。
- 6-磷酸果糖激酶-1 /
- 鼻咽肿瘤 /
- 糖酵解 /
- 淋巴结转移
Abstract: Objective:The aim of this study is to investigate the expression and clinical significance of 6-phosphofructo-1-kinase in nasopharyngeal carcinoma biopsy samples.Method:Sixty-one biopsy samples were detected, including 41 tissues samples from patients with nasopharyngeal carcinoma and 20 tissues samples from patients with chronic nasopharyngitis as control group. 6-phosphofructo-1-kinase mRNA expression was detected by RT-PCR.Result:It was observed that the expression levels of 6-phosphofructo-1-kinase mRNA in nasopharyngeal carcinoma tissues were higher than in the chronic inflammatory tissues. And the expression levels in patients with lymph node metastasis were higher than without lymph nod metastasis.Conclusion:6-phosphofructo-1-kinase may be a marker in occurrence and metastasis of nasopharyngeal carcinoma.
- 6-phosphofructo-1-kinase /
- nasopharyngeal carcinoma /
- glycolysis /
- lymph node metastasis

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