Expression of HIF-1α and VEGF in human laryngeal carcinoma and its relationship with angiogenes
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摘要: 目的:研究缺氧诱导因子-1α(HIF-1α)及血管内皮生长因子(VEGF)在喉癌组织中的表达程度及其与喉癌临床病理学特征之间的关系,并探讨HIF-1α及VEGF在肿瘤血管生成中的作用。为喉癌的发生机制提供理论基础,并为喉癌的早期诊断提供新思路。方法:试验分组:60例喉癌标本及20例癌旁正常组织。采用免疫组织化学方法检测HIF-1α及VEGF表达情况,并用CD105标记血管内皮细胞,计算微血管密度(MVD)。结果:60例喉癌组织中,HIF-1α、VEGF阳性表达率分别为71.67%(43/60)、65.00%(39/60),MVD平均值为33.82±10.15;20例癌旁正常组织中,HIF-1α、VEGF阳性表达率分别为10%(2/20)、20%(4/20),MVD平均值为21.48±6.84。喉癌组织中HIF-1α、VEGF阳性表达率显著高于正常喉组织。HIF-1α在喉癌组织中的阳性表达率与癌组织的临床分期、分化程度和淋巴结转移密切相关。VEGF阳性表达率与临床分期及有无淋巴结转移相关,而与组织分化程度无关。HIF-1α与VEGF表达一致符合率为56.67%(34/60),两者表达呈明显正相关。HIF-1α、VEGF均阳性的喉癌组织MVD高于两者均为阴性者,差异有统计学意义(P<0.01)。结论:喉癌组织中存在HIF-1α蛋白及VEGF的过表达,HIF-1α、VEGF与MVD在喉癌中的表达呈正相关,协同促进肿瘤新血管形成。[JP]Abstract: Objective:The aim of this study is to investigate the expression of hypoxia inducible factor-1α(HIF-1α),vascular endothelial growth factor(VEGF)in laryngeal carcinoma and its relations to clinical and pathophysiological characteristics,and determine the effect of HIF-1α,VEGF in the processing of angiogenesis.To offer the theoretical basis for the pathogenesis of laryngeal carcinoma and the new ideas for early diagnosis of laryngeal carcinoma.Method:The major was divided into two groups:60 specimens of laryngeal carcinoma and 20 specimens of normal tissues beside the carcinoma.The expression of HIF-1α、VEGF was detected in 60 specimens of laryngeal carcinoma tissues and 20 specimens of normal tissues beside the carcinoma as controls by immunohistochemical staining technique.Microvessel was stained by antifactor CD105 antibody to analyse microvessel density.Result:Positive rate of HIF-1α and VEGF protein expression was 71.67%(43/60)and 65.00%(39/60)respectively in laryngeal carcinoma tissues,which was significantly higher than that in normal laryngeal tissues(10.00% and 20.00%,respectively).HIF-1α expression was closely related to P-TNM stage、the grade of cell differentiation and lymph node status; VEGF expression was closely related to P-TNM stage and lymph node status.The coincidence rate of the expression of HIF-1α and VEGF was 56.67%(34/60),showing a close relation between them. MVD was much higher in tumor with HIF-1α(+)/VEGF(+) than that in tumor with HIF-1α(-)/VEGF(-)(P<0.01).Conclusion:HIF-1α protein and VEGF was over-expressed in laryngeal carcinoma.The positive expression of HIF-1α、VEGF and MVD in laryngeal carcinoma have a synergistic effect on the neovascularization of the tumor.
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