喉鳞状细胞癌中细胞周期蛋白E和p27异常表达的临床意义

柴大敏; 鲍正齐; 胡建国; 马莉; 冯振中; 陶仪声

doi:10.13201/j.issn.1001-1781.2014.03.007

喉鳞状细胞癌中细胞周期蛋白E和p27异常表达的临床意义

- 1 蚌埠医学院第一附属医院病理科(安徽蚌埠, 233000);
- 2 蚌埠医学院第一附属医院骨科;
- 3 蚌埠医学院科研中心
基金项目:
国家自然科学基金 (No:81171465)

安徽省教育厅自然科学研究项目 (No:KJ2013B141)

蚌埠市科技计划项目 (No:20120317)

详细信息

通讯作者: 陶仪声, E-mail:tys101041@126.com

中图分类号: R739.65

收稿日期: 2012-11-17

Aberrant expression of CyclinE and p27 in laryngeal squamous cell carcinoma and the clinical significance

- 1 Department of Pathology, First Affiliated Hospital, Bengbu Medical College, Bengbu, 233000, China;
- 2 Department of Orthopedics, First Affiliated Hospital, Bengbu Medical College;
- 3 Scientific Research Center, Bengbu Medical College

More Information

Corresponding author: TAO Yisheng, E-mail: tys101041@126.com

Received Date: 17 November 2012

摘要

摘要: 目的:探讨喉鳞状细胞癌(LSCC)中细胞周期蛋白E (CyclinE)和p27蛋白的表达情况及其临床意义,旨在寻找影响LSCC患者预后的新指标。方法:采用免疫组织化学Elivision^TM法检测160例LSCC和20例正常喉黏膜组织(NLT)中CyclinE和p27的表达情况;统计分析CyclinE和p27蛋白之间及其二者与LSCC患者临床病理因素和总体生存期之间的关系。结果:LSCC组织中CyclinE和p27阳性率分别为62.50%(100/160)和41.25%(66/160),NLT中CyclinE和p27阳性率分别为35%(7/20)和70%(14/20)(P<0.05); CyclinE和p27与LSCC患者淋巴结转移和PTNM分期有关(P<0.05),与年龄、性别及肿瘤直径、部位和分化程度无关(P>0.05);CyclinE和p27呈显著负相关,r=-0.767(P<0.05);Cox多因素分析显示淋巴结转移、PTNM分期、CyclinE和p27是影响LSCC患者预后的独立危险因素。Kaplan-Meier生存分析显示LSCC患者的总体生存率为36.9% (P<0.05);CyclinE阳性组患者5年生存率为8%,阴性组为80%(P<0.05);而p27蛋白阳性组患者5年生存率为77.27%,阴性组为5.32%(P<0.05)。结论:CyclinE基因的激活同时伴有p27基因的失活可能是LSCC发生发展和侵袭转移的分子基础,联合检测CyclinE和p27对判断LSCC患者预后有重要意义。
- 喉肿瘤 /
- 细胞周期蛋白E /
- p27 /
- 免疫组织化学 /
- 预后
Abstract: Objective:To explore new hallmarks affecting the prognosis of patients with laryngeal squamous cell carcinoma(LSCC) via investigating the expression of CyclinE and p27 in LSCC tissues.Method:The expression of CyclinE and p27 was detected via Elivision^TM immunohistochemical staining in 160 LSCC tissues and 20 normal laryngeal tissues(NLT). The relationship between CyclinE/ p27 and LSCC/ NLT was analyzed via Log-rank analysis. The relationship of CyclinE and p27 protein was statistically analyzed by spearman correlation analysis. The relationship between CyclinE/ p27 and clinical-pathology-factors of patients with LSCC, such as age, gender, tumor site, diameter, differentiation, lymph node metastasis and PTNM stage were analyzed by Chi-square test. The relationship between clinical-pathology-factors, CyclinE, p27 and overall survival time of patients with LSCC was analyzed via Cox multiplicity and Kaplan-Meier survival analysis. A significant difference was recognized by P<0.05.Result:In LSCC the positive rates of CyclinE and p27 protein was 62.50% and 41.25% respectively(P<0.05). In NLT the positive rates of CyclinE and p27 protein was 35% and 70% respectively(P<0.05). The expression of CyclinE or p27 protein was closely correlated with lymph node metastasis, PTNM stage of patients with LSCC(P<0.05). The expression of CyclinE and p27 had no significant correlations with patients' gender, age and tumor site, diameter differentiation(P>0.05 for all). A negative correlation was found between the expression of CyclinE and p27 protein, r=-0.767(P<0.05). Kaplan-Meier survival analysis showed that the overall survival rate of patients with LSCC was 36.9%(P<0.05). The 5-year survival rate in positive group of Cy-clinE was 8%, in negative group was 80%(P<0.05). On the contrary, the 5-year survival rate of patients with LSCC in positive group of p27 protein was 77.27%, the rate was 5.32% in negative group(P<0.05). Cox multivariate regression analysis revealed that lymph node metastasis, PTNM stage, CyclinE and p27 were independent risk factors of prognosis for patients with LSCC.Conclusion:It is the molecular basis underlying the development and invasion/ metastasis of LSCC that activation of CyclinE gene accompanying inactivation of p27 gene. It is very important of co-detecting CyclinE and p27 protein to predict the prognosis of patients with LSCC.
- laryngeal neoplasms /
- cycline /
- p27 /
- immunohistochemistry /
- prognosis

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