弥漫硬化型和普通型甲状腺乳头状癌中黏蛋白1和p63蛋白的表达及意义

郝亚琳; 金城; 王家东

doi:10.13201/j.issn.1001-1781.2013.09.005

弥漫硬化型和普通型甲状腺乳头状癌中黏蛋白1和p63蛋白的表达及意义

- 上海交通大学医学院附属仁济医院耳鼻咽喉头颈外科(上海, 200001)

详细信息

通讯作者: 王家东,E-mail:drjiadongw@yahoo.com.cn

中图分类号: R739.6

收稿日期: 2012-11-23

Expression and clinical significance of Muc1,p63 protein in diffuse sclerosing variant of papillary thyroid carcinoma and conventional papillary thyroid carcinoma

- Department of Otolaryngology-Head and Neck Surgery, Renji Hospital, Medical College of Shanghai Jiao Tong University, Shanghai, 200001, China

More Information

Corresponding author: WANG Jiadong, E-mail: drjiadongw@yahoo.com.cn

Received Date: 23 November 2012

摘要

摘要: 目的:探讨黏蛋白1(Muc1)和p63蛋白在弥漫硬化型甲状腺乳头状癌(DSVPTC)及普通型甲状腺乳头状癌(CPTC)中的表达及意义。方法:运用免疫组织化学(SP法)检测30例DSVPTC(实验组)、30例CPTC(对照组)中Muc1和p63蛋白的表达情况。结果:①实验组和对照组中Muc1的阳性表达率分别为76.67%(23/30)和53.33%(16/30),SP染色表现为细胞膜或细胞质内棕黄色或棕褐色颗粒,2组间比较差异有统计学意义(P＜0.05);p63在2组中的阳性表达率分别为80%(24/30)和43.33%(13/30),SP染色表现为细胞核内棕黄色或棕褐色颗粒,2组间比较差异有统计学意义(P＜0.05)。②实验组和对照组颈淋巴结转移率分别为50%(15/30)和20%(6/30),2组之间差异有统计学意义(P＜0.05)。③所有病例中,有颈淋巴结转移组(21例)和无颈淋巴转移组(39例)中Muc1的阳性表达率分别为85.71%(18/21)、53.85%(21/39),2组之间差异有统计学意义(P＜0.05);p63的阳性表达率分别为95.24%(20/21)、43.59%(17/39),2组之间差异有统计学意义(P＜0.01)。④Spearman等级相关分析显示,Muc1和p63蛋白在实验组和对照组中的表达均呈正相关(r=0.530、0.386,均P<0.05)。结论:①Muc1、p63蛋白在DSVPTC中高表达,在CPTC中相对低表达;确诊时DSVPTC颈部淋巴结转移的发生率较CPTC高,提示DSVPTC是甲状腺乳头状癌(PTC)中恶性度较高的一种亚型。②Muc1、p63的异常表达可能在促使PTC的进展和转移中有重要意义,可作为PTC恶性行为的预测因子。③Muc1和p63协同作用可能共同促进了PTC恶性细胞的增殖和侵袭转移。
- 弥漫硬化型,普通型,甲状腺乳头状癌 /
- 黏蛋白1 /
- p63
Abstract: Objective: To investigate the expression and clinical significance of Muc1,p63 protein in diffuse sclerosing variant of papillary thyroid carcinoma and conventional papillary thyroid carcinoma.Method: Immunohistochemistry(SP) was used to detect the expressions of Muc1,p63 protein in 30 samples of DSVPTC(experiment group) and 30 samples of CPTC(control group).Patients in two groups were matched in age,dender,tumor side,tumor size and date of diagnosis.Result: ①The positive rate of Muc1 in DSVPTC and CPTC was 76.67%(23/30)and 53.33%(16/30)respectively,immunohistochemical staining expressed as brown or tan particles in the membrane or the cytoplasm,with a significant difference between the two groups(P＜0.05). The positive rate of p63 in DSVPTC and CPTC was 80%(24/30)and 43.33%(13/30)respectively,immunohistochemical stainning expressed as a brown or tan particles in the muclei,with a significant difference between the two groups(P＜0.05).②Cervical lymph node metastasis rate in DSVPTC and CPTC was 50%(15/30)and 20%(6/30)respectively,with a significant difference between the two groups(P＜0.05).③In All cases,the positive rate of Muc1 in cervical lymph node metastasis group(21 cases) and without metastasis group(39 cases)was 85.71%(18/21) and 53.85%(21/39) respectively,with a significant difference between the two groups(P＜0.05);the positive rate of p63 was 95.24%(20/21)and 43.59%(17/39)respectively,with a significant difference between the two groups(P＜0.01).④Spearman rank correlation analysis showed that expression of Muc1 and p63 were positively correlated in both groups(r=0.530,0.386,P<0.05).Conclusion: ①There are high expression of Muc1 and p63 protein in DSVPTC, and relatively low expression in CPTC,DSVPTC have a higher rate of cervical lymph node metastasis at the time of being diagnosed, compared to the CPTC.These results show that DSVPTC is a more biologically aggressive variant of the PTC.②Abnormal expression of Muc1 and p63 may be important to promote the progression and metastasis of PTC,thus they can be used as predictors of maligant behavior in PTC.③Muc1 and p63 may be synergistically promote proliferation and invasion metastasis of the PTC malignant cell.
- diffuse sclerosing variant,conventional,papillary thyroid carcinoma /
- Muc1 /
- p63

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