Analysis on expression of Survivin and PTEN in vocal cords polyps,papilloma of larynx and laryngeal squamous cell carcinoma
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摘要: 目的:研究声带息肉、成人型喉乳头状瘤和喉鳞状细胞癌3种疾病中Survivin和PTEN的表达特点,以了解喉鳞状细胞从增生到癌变的可能机制。方法:选取符合临床与病理诊断的声带息肉患者18例、成人型喉乳头状瘤患者10例和喉鳞状细胞癌患者18例,将每个患者的病理蜡块标本各连续切片2张,分别进行Survivin和PTEN免疫组织化学检测。比较致癌基因Survivin和抑癌基因PTEN在这3种疾病同一区域鳞状上皮细胞中的表达差异。结果:声带息肉的Survivin和PTEN阳性率均显著低于喉乳头状瘤和喉鳞状细胞癌(P＜0.05),而喉乳头状瘤的Survivin和PTEN阳性率均低于喉鳞状细胞癌,但均没有显著性差异(P＞0.05),Survivin和PTEN形成近似声带息肉＜成人型喉乳头状瘤＜喉鳞状细胞癌的表达趋势。在声带息肉、喉乳头状瘤及喉鳞状细胞癌3种疾病中,PTEN的阳性率均显著低于Survivin(P＜0.05)。结论:Survivin和PTEN在喉良性增生性疾病的鳞状细胞中部分表达,而在良性和恶性肿瘤中过度表达,体现出随恶性程度增加而逐渐升高的表达趋势,Survivin和PTEN虽然功能相反,但作用机制不同,非对立消长关系,PTEN体现出一定的竞争表达特点,并从属于Survivin的表达,两者的统一部分决定了喉鳞状细胞的恶变程度。Abstract: Objective: Through exploring the differentiation on positive expressing rates between oncogene Survivin and tumor-suppresser gene PTEN(phosphatase and tensin homolog deleteted on chromosome Ten) on vocal cord polyps(VCP), adult type laryngeal papilloma (ALP), and Laryngeal Squamous Cell Carcinoma (LSCC), to reveal the mechanism in canceration of human laryngeal squamous cells, from benign proliferative lesion, benign tumor to malignant tumor in larynx.Method: After picking out 18 cases of VCP, 10 cases of ALP, and 18 cases of LSCC for immunohistochemical process of Survivin and PTEN with two continuous section preparations, the differentiations of positive expression rates of Survivin and PTEN in the same human laryngeal squamous cells areas among three diseases were compared.Conclusion: The positive expressing rates of Survivin and PTEN in VCP were obviously more lower than in ALP and LSCC(P<0.05), which showed no obvious difference between each other(P＞0.05). The positive expressing rates of Survivin were always higher than PTEN in VCP, ALP and LSCC evidently(P＜0.05).Conclusion: PTEN, expressing for competition purpose, shows a subordinative relationship with Survivin. Although they have opposite functions in determine whether the canceration of laryngeal squamous cells take place or not, Survivin is always playing the leading role and making predominant impact on development of benign proliferative lesion, benign and malignant tumor in larynx.
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Key words:
- vocal cords polyps /
- laryngeal neoplasm /
- Survivin /
- PTEN
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