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摘要: 目的：本研究拟探讨桥粒芯胶蛋白-1与头颈部鳞状细胞癌发生及预后的相关性。方法：本研究通过对NCBI数据库GEO获取的基因芯片数据进行统计学分析，并且采用头颈部鳞状细胞癌组织芯片予以验证。免疫组织化学的方法检测了组织芯片中芯胶蛋白-1的表达，并采用统计学方法分析了芯胶蛋白-1的表达与头颈部鳞状细胞癌发生、分化、预后等的相关性。结果：对GEO获取的芯片数据分析发现芯胶蛋白-1在头颈部鳞状细胞癌中表达增高，且表达增高的患者预后较差。组织芯片的验证实验显示结果与GEO数据库获取数据分析结果基本一致，在头颈部鳞状细胞癌中DSC1的表达显著高于正常组织，且在低分化头颈部鳞状细胞癌中表达高于高分化鳞状细胞癌。结论：桥粒芯胶蛋白-1的增高可促进头颈部鳞状细胞癌的发生，且与肿瘤分化相关。此外，其表达增高提示患者预后不良。Abstract: Objective: To explore the correlation between the expression of desmocollin-1and the tumorigenesis and prognosis of HNSCC.Method: Five datasets of HNSCC from the GEO were analyzed. A tumor tissue microassay was chosen for further test. The expression of DSC1 of TMA was detected by immunohistochemical staining.Result: The expression of DSC1 was significantly increased in HNSCC. Meanwhile, the expression of DSC1 was much higher in poor-differentiated tumor than the well-differentiated tumor in HNSCC. What's more, the HNSCC patients with lower expression of DSC1 had better outcomes.Conclusion: The results were according with the results of statistical analysis with the bioinformatics data from GEO, indicating that DSC1 significant correlated with the tumorigenesis and prognosis of HNSCC.
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Key words:
- desmocollin-1 /
- HNSCC /
- prognosis /
- tissue microassay /
- NCBI database
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